PMID- 36500895
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221221
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 12
IP  - 23
DP  - 2022 Dec 1
TI  - Identification of Zirconia Particle Uptake in Human Osteoblasts by ToF-SIMS 
      Analysis and Particle-Size Effects on Cell Metabolism.
LID - 10.3390/nano12234272 [doi]
LID - 4272
AB  - As the use of zirconia-based nano-ceramics is rising in dentistry, the 
      examination of possible biological effects caused by released nanoparticles on 
      oral target tissues, such as bone, is gaining importance. The aim of this 
      investigation was to identify a possible internalization of differently sized 
      zirconia nanoparticles (ZrNP) into human osteoblasts applying Time-of-Flight 
      Secondary Ion Mass Spectrometry (ToF-SIMS), and to examine whether ZrNP exposure 
      affected the metabolic activity of the cells. Since ToF-SIMS has a low probing 
      depth (about 5 nm), visualizing the ZrNP required the controlled erosion of the 
      sample by oxygen bombardment. This procedure removed organic matter, uncovering 
      the internalized ZrNP and leaving the hard particles practically unaffected. It 
      was demonstrated that osteoblasts internalized ZrNP within 24 h in a 
      size-dependent manner. Regarding the cellular metabolic activity, metabolization 
      of alamarBlue by osteoblasts revealed a size- and time-dependent unfavorable 
      effect of ZrNP, with the smallest ZrNP exerting the most pronounced effect. These 
      findings point to different uptake efficiencies of the differently sized ZrNP by 
      human osteoblasts. Furthermore, it was proven that ToF-SIMS is a powerful 
      technique for the detection of zirconia-based nano/microparticles that can be 
      applied for the cell-based validation of clinically relevant materials at the 
      nano/micro scale.
FAU - Welle, Alexander
AU  - Welle A
AD  - Karlsruhe Nano Micro Facility (KNMF), Karlsruhe Institute of Technology (KIT), 
      76344 Eggenstein-Leopoldshafen, Germany.
AD  - Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 
      76344 Eggenstein-Leopoldshafen, Germany.
FAU - Rabel, Kerstin
AU  - Rabel K
AUID- ORCID: 0000-0001-7253-4165
AD  - Department of Prosthetic Dentistry, Medical Center-University of Freiburg, 
      Faculty of Medicine, University of Freiburg, 79106 Freiburg im Breisgau, Germany.
FAU - Schwotzer, Matthias
AU  - Schwotzer M
AUID- ORCID: 0000-0003-2433-1181
AD  - Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), 
      76344 Eggenstein-Leopoldshafen, Germany.
FAU - Kohal, Ralf Joachim
AU  - Kohal RJ
AUID- ORCID: 0000-0001-7095-4190
AD  - Department of Prosthetic Dentistry, Medical Center-University of Freiburg, 
      Faculty of Medicine, University of Freiburg, 79106 Freiburg im Breisgau, Germany.
FAU - Steinberg, Thorsten
AU  - Steinberg T
AUID- ORCID: 0000-0003-1924-2694
AD  - Department of Oral Biotechnology, Center for Dental Medicine, Medical 
      Center-University of Freiburg, Faculty of Medicine, University of Freiburg, 79106 
      Freiburg im Breisgau, Germany.
FAU - Altmann, Brigitte
AU  - Altmann B
AD  - Department of Prosthetic Dentistry, Medical Center-University of Freiburg, 
      Faculty of Medicine, University of Freiburg, 79106 Freiburg im Breisgau, Germany.
AD  - G.E.R.N. Research Center for Tissue Replacement, Regeneration & Neogenesis, 
      Department of Prosthetic Dentistry, Medical Center-University of Freiburg, 
      Faculty of Medicine, University of Freiburg, 79085 Freiburg im Breisgau, Germany.
LA  - eng
PT  - Journal Article
DEP - 20221201
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC9736599
OTO - NOTNLM
OT  - Time-of-Flight Secondary Ion Mass Spectrometry
OT  - alamarBlue assay
OT  - human alveolar bone
OT  - nano/microparticles
OT  - zirconia
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/12 06:00
MHDA- 2022/12/12 06:01
PMCR- 2022/12/01
CRDT- 2022/12/11 01:30
PHST- 2022/10/25 00:00 [received]
PHST- 2022/11/22 00:00 [revised]
PHST- 2022/11/23 00:00 [accepted]
PHST- 2022/12/11 01:30 [entrez]
PHST- 2022/12/12 06:00 [pubmed]
PHST- 2022/12/12 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - nano12234272 [pii]
AID - nanomaterials-12-04272 [pii]
AID - 10.3390/nano12234272 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2022 Dec 1;12(23):4272. doi: 10.3390/nano12234272.