PMID- 36502299
OWN - NLM
STAT- MEDLINE
DCOM- 20230711
LR  - 20230718
IS  - 1878-3619 (Electronic)
IS  - 0959-2989 (Linking)
VI  - 34
IP  - 4
DP  - 2023
TI  - Experimental study on co-culture of DiI-labeled rat bone marrow mesenchymal stem 
      cells and neonatal rat cardiomyocytes to induce differentiation into 
      cardiomyocyte-like cells.
PG  - 319-330
LID - 10.3233/BME-221429 [doi]
AB  - BACKGROUND: Myocardial infarction is a serious clinical disease with high 
      mortality and poor prognosis. Cardiomyocytes (CMs) have limited regeneration 
      abilities after ischemic injury. Their growth and differentiation can be enhanced 
      by contact co-culture with stem cells. OBJECTIVE: The aim was to study the 
      contact co-culture of Dil-labeled bone marrow mesenchymal stem cells (BMSCs) and 
      CMs for inducing differentiation of CMs from stem cells for treating myocardial 
      infarction. METHODS: After contact co-culture, the differentiation of BMSCs into 
      CMs was analyzed qualitatively by detecting myocardial markers (cardiac troponin 
      T and alpha-smooth muscle actin) using immunofluorescence and quantitatively using 
      flow cytometry. To examine the mechanism, possible gap junctions between BMSCs 
      and CMs were analyzed by detecting gap junction protein connexin 43 (Cx43) 
      expression in BMSCs using immunofluorescence. The functionality of gap junctions 
      was analyzed using dye transfer experiments. RESULTS: The results revealed that 
      BMSCs in contact with CMs exhibited myocardial markers and a significant increase 
      in differentiation rate (P < 0.05); they also proved the existence and function 
      of gap junctions between BMSCs and CMs. CONCLUSIONS: It was shown that contact 
      co-culture can induce Dil-labeled BMSCs to differentiate into CM-like cells and 
      examined the principle of gap junction-mediated signaling pathways involved in 
      inducing stem cells to differentiate into cardiomyocytes.
FAU - Mu, Junsheng
AU  - Mu J
AD  - Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical 
      University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, 
      China.
FAU - Zhang, Zichang
AU  - Zhang Z
AD  - Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical 
      University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, 
      China.
FAU - Zhou, Fan
AU  - Zhou F
AD  - Department of Ultrasound, The Third Medical Center of PLA General Hospital, 
      Beijing, China.
FAU - Zheng, Jianwei
AU  - Zheng J
AD  - Heart Center of Sunshine Union Hospital, Weifang, China.
FAU - Bo, Ping
AU  - Bo P
AD  - Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical 
      University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, 
      China.
FAU - You, Bin
AU  - You B
AD  - Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical 
      University, Beijing Institute of Heart Lung and Blood Vessel Diseases, Beijing, 
      China.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biomed Mater Eng
JT  - Bio-medical materials and engineering
JID - 9104021
SB  - IM
MH  - Rats
MH  - Animals
MH  - Myocytes, Cardiac
MH  - Coculture Techniques
MH  - Animals, Newborn
MH  - Bone Marrow Cells/metabolism
MH  - Cell Differentiation/physiology
MH  - *Mesenchymal Stem Cells
MH  - Cells, Cultured
MH  - *Myocardial Infarction
OTO - NOTNLM
OT  - DiI labeling in vitro
OT  - Stem cell therapy
OT  - bone marrow mesenchymal stem cells
OT  - cardiomyocytes
OT  - contact co-culture
EDAT- 2022/12/12 06:00
MHDA- 2023/07/11 06:41
CRDT- 2022/12/11 04:42
PHST- 2023/07/11 06:41 [medline]
PHST- 2022/12/12 06:00 [pubmed]
PHST- 2022/12/11 04:42 [entrez]
AID - BME221429 [pii]
AID - 10.3233/BME-221429 [doi]
PST - ppublish
SO  - Biomed Mater Eng. 2023;34(4):319-330. doi: 10.3233/BME-221429.