PMID- 36504328
OWN - NLM
STAT- MEDLINE
DCOM- 20230118
LR  - 20230118
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 107
IP  - 2-3
DP  - 2023 Feb
TI  - The viability of spores is the key factor for microbial induced calcium carbonate 
      precipitation.
PG  - 543-552
LID - 10.1007/s00253-022-12319-w [doi]
AB  - While previous studies mainly focused on the total number of spores as an index 
      to predict the calcium precipitation activity (CPA) of bacterial strains, the 
      effect of viability of spores on microbial-induced calcium precipitation (MICP) 
      has remained highly ignored. Therefore, for the first time, we have attempted to 
      optimize the sporulation process in terms of viable spore production and, most 
      importantly, aimed to build a correlation between viable spores and CPA. The 
      results have shown that for the sporulation of Bacillus sp. H4, starch and 
      peptone are the optimal carbon and nitrogen sources, respectively. One gram per 
      liter of sodium chloride promotes CPA and production of viable spores, whereas an 
      increase of sodium chloride concentration beyond 8 g L(-1) significantly reduces 
      CPA without reducing the quantity of viable spores. Exogenous conditions such as 
      seed age, inoculation quantity, and liquid volume only pose slight influence on 
      the sporulation and CPA. Conclusively, the spores produced under optimized 
      conditions are more morphologically uniform and display a 20% increase in CPA 
      compared to pre-optimized spores. Furthermore, by combining the results of 
      heatmap analysis, it can be concluded that not only the quantity, but also the 
      quality of viable spores is important for bacterial strain to develop high CPA 
      and effective MICP process. This study sheds light on the breadth of 
      biomineralization activity based on viable spores and is an imperative step 
      toward the intelligible design of MICP-based engineering solutions. KEY POINTS: * 
      Viability of spores is a key controlling factor in calcium precipitation activity 
      (CPA). * Spores produced under optimized conditions display a 20% increase in 
      CPA. * Quality of viable spores is imperative for bacterial strains to develop 
      high CPA.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Huang, Rong
AU  - Huang R
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, 
      College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, 
      China.
FAU - Lu, Yuanyi
AU  - Lu Y
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, 
      College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, 
      China.
FAU - Ahmad, Muhammad Arslan
AU  - Ahmad MA
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, 
      College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, 
      China.
AD  - College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen, 
      518060, China.
FAU - Zhang, Jinlong
AU  - Zhang J
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, 
      College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, 
      China.
FAU - Deng, Xu
AU  - Deng X
AUID- ORCID: 0000-0001-8783-1461
AD  - Shenzhen Key Laboratory of Marine Bioresource and Eco-Environmental Science, 
      College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, 
      China. dengxu@szu.edu.cn.
LA  - eng
GR  - 2018YFE0125000/national key research and development project/
GR  - 2022YFE0109300/national key research and development project/
GR  - 52178229/national natural science foundation/
GR  - No. 20200812162503001/Shenzhen R&D project of China/
PT  - Journal Article
DEP - 20221212
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - H0G9379FGK (Calcium Carbonate)
RN  - SY7Q814VUP (Calcium)
RN  - 451W47IQ8X (Sodium Chloride)
SB  - IM
MH  - *Calcium Carbonate
MH  - Calcium
MH  - Sodium Chloride
MH  - Spores, Bacterial
MH  - *Bacillus
MH  - Bacteria
MH  - Chemical Precipitation
MH  - Microbial Viability
OTO - NOTNLM
OT  - Bacillus sp. H4
OT  - Calcium precipitation activity
OT  - Optimization
OT  - Self-healing concrete
OT  - Viable spores
EDAT- 2022/12/13 06:00
MHDA- 2023/01/19 06:00
CRDT- 2022/12/12 10:28
PHST- 2022/10/25 00:00 [received]
PHST- 2022/11/26 00:00 [accepted]
PHST- 2022/11/22 00:00 [revised]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2023/01/19 06:00 [medline]
PHST- 2022/12/12 10:28 [entrez]
AID - 10.1007/s00253-022-12319-w [pii]
AID - 10.1007/s00253-022-12319-w [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2023 Feb;107(2-3):543-552. doi: 
      10.1007/s00253-022-12319-w. Epub 2022 Dec 12.