PMID- 36504859
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221220
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Analysis of reproduction-related transcriptomes on 
      pineal-hypothalamic-pituitary-ovarian tissues during estrus and anestrus in Tan 
      sheep.
PG  - 1068882
LID - 10.3389/fvets.2022.1068882 [doi]
LID - 1068882
AB  - Seasonal estrus is an important factor limiting the fertility of some animals 
      such as sheep. Promoting estrus in the anestrus season is one of the major ways 
      in improving the fecundity of seasonally breeding animals. The 
      pineal-hypothalamus-pituitary-ovary (PHPO) axis plays a decisive role in 
      regulating animal reproduction. However, the molecular mechanisms by which the 
      PHPO axis regulates seasonal reproduction in animals are not well understood, 
      especially in Tan sheep. To this end, we collected pineal, hypothalamus, 
      pituitary and ovary tissues from Tan sheep during estrus and anestrus for 
      RNA-Sequencing, and performed bioinformatics analysis on the entire regulatory 
      axis of the pineal-hypothalamic-pituitary-ovary (PHPO). The results showed that 
      940, 1,638, 750, and 971 DEGs (differentially expressed genes, DEGs) were 
      identified in pineal, hypothalamus, pituitary and ovary, respectively. GO 
      analysis showed that DEGs from PHPO axis-related tissues were mainly enriched in 
      "biological processes" such as transmembrane transport, peptide and amide 
      biosynthesis and DNA synthesis. Meanwhile, KEGG enrichment analysis showed that 
      the bile acid secretion pathway and the neuroactive ligand-receptor interaction 
      pathway were significantly enriched. Additionally, four potential candidate genes 
      related to seasonal reproduction (VEGFA, CDC20, ASPM, and PLCG2) were identified 
      by gene expression profiling and protein-protein interaction (PPI) analysis. 
      These findings will contribute to be better understanding of seasonal 
      reproduction regulation in Tan sheep and will serve as a useful reference for 
      molecular breeding of high fertility Tan sheep.
CI  - Copyright (c) 2022 Wei, Kang, Yang, Wang, Dai, Guo, Ma, Li, Zhao and Dan.
FAU - Wei, Shihao
AU  - Wei S
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Kang, Xiaolong
AU  - Kang X
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Yang, Chaoyun
AU  - Yang C
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Wang, Feng
AU  - Wang F
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Dai, Tianshu
AU  - Dai T
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Guo, Xingru
AU  - Guo X
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Ma, Ziming
AU  - Ma Z
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Li, Chenglong
AU  - Li C
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Zhao, Hongxi
AU  - Zhao H
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
FAU - Dan, Xingang
AU  - Dan X
AD  - Key Laboratory of Ruminant Molecular and Cellular Breeding, School of 
      Agriculture, Ningxia University, Yinchuan, China.
LA  - eng
PT  - Journal Article
DEP - 20221124
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9729709
OTO - NOTNLM
OT  - PHPO axis
OT  - Tan sheep
OT  - anestrus
OT  - estrus
OT  - seasonal reproduction
OT  - transcriptome
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/13 06:00
MHDA- 2022/12/13 06:01
PMCR- 2022/01/01
CRDT- 2022/12/12 10:50
PHST- 2022/10/13 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2022/12/12 10:50 [entrez]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2022/12/13 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.1068882 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Nov 24;9:1068882. doi: 10.3389/fvets.2022.1068882. 
      eCollection 2022.