PMID- 36505238
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221221
IS  - 2296-861X (Print)
IS  - 2296-861X (Electronic)
IS  - 2296-861X (Linking)
VI  - 9
DP  - 2022
TI  - A low carbohydrate diet high in fish oil and soy protein delays inflammation, 
      hematopoietic stem cell depletion, and mortality in miR-146a knock-out mice.
PG  - 1017347
LID - 10.3389/fnut.2022.1017347 [doi]
LID - 1017347
AB  - Since our previous studies found a low carbohydrate (CHO) diet containing soy 
      protein and fish oil (15%Amylose/Soy/FO) significantly reduced lung and breast 
      cancer in mice we asked herein if this low CHO diet could also delay the onset of 
      myeloid malignancies. To test this we employed a miR-146a knock-out (KO) mouse 
      model and found the 15%Amylose/Soy/FO diet increased their median lifespan by 8.5 
      month, compared to these mice on a Western diet. This was associated with 
      increased lymphocytes and reduced monocytes, granulocytes, blood glucose and 
      insulin levels. Inflammatory cytokine/chemokine studies carried out with 
      6-month-old mice, before any signs of illness, revealed the 15%Amylose/Soy/FO 
      diet significantly reduced pro-inflammatory cytokines. This low CHO diet also led 
      to an increase in plasma beta-hydroxybutyrate and in liver fatty acid synthase 
      levels. This, together with higher liver carnitine palmitoyltransferase I levels 
      suggested that the 15%Amylose/Soy/FO diet was causing a systemic metabolic shift 
      from glucose to fatty acids as an energy source. Lastly, we found the 
      15%Amylose/Soy/FO diet resulted in significantly higher numbers of primitive 
      hematopoietic stem cells (HSCs) in the bone marrow of 6-month-old mice than those 
      fed a Western diet. Taken together, these results suggest a 15%Amylose/Soy/FO 
      diet reduces chronic inflammation and increases fatty acid oxidation and that 
      this, in turn, may prevent HSC proliferation and exhaustion, thereby delaying 
      myeloid malignancy-induced death of miR-146a KO mice. We suggest a low CHO diet 
      containing soy protein and fish oil could be beneficial in reducing the risk of 
      myeloid malignancies in patients with low miR-146a levels.
CI  - Copyright (c) 2022 Elisia, Kowalski, Yeung, Wong, Grants, Karsan and Krystal.
FAU - Elisia, Ingrid
AU  - Elisia I
AD  - The Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada.
FAU - Kowalski, Sara
AU  - Kowalski S
AD  - The Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada.
FAU - Yeung, Michelle
AU  - Yeung M
AD  - The Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada.
FAU - Wong, Jennifer
AU  - Wong J
AD  - The Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada.
FAU - Grants, Jennifer M
AU  - Grants JM
AD  - Canada's Michael Smith Genome Sciences Centre, BC Cancer Research Centre, 
      Vancouver, BC, Canada.
FAU - Karsan, Aly
AU  - Karsan A
AD  - Canada's Michael Smith Genome Sciences Centre, BC Cancer Research Centre, 
      Vancouver, BC, Canada.
FAU - Krystal, Gerald
AU  - Krystal G
AD  - The Terry Fox Laboratory, BC Cancer Research Centre, Vancouver, BC, Canada.
LA  - eng
PT  - Journal Article
DEP - 20221124
PL  - Switzerland
TA  - Front Nutr
JT  - Frontiers in nutrition
JID - 101642264
PMC - PMC9729559
OTO - NOTNLM
OT  - HSC exhaustion
OT  - inflammation
OT  - low carbohydrate diet
OT  - miR-146a
OT  - stem cell
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/13 06:00
MHDA- 2022/12/13 06:01
PMCR- 2022/01/01
CRDT- 2022/12/12 10:56
PHST- 2022/08/11 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2022/12/12 10:56 [entrez]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2022/12/13 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fnut.2022.1017347 [doi]
PST - epublish
SO  - Front Nutr. 2022 Nov 24;9:1017347. doi: 10.3389/fnut.2022.1017347. eCollection 
      2022.