PMID- 36509010
OWN - NLM
STAT- MEDLINE
DCOM- 20230123
LR  - 20230123
IS  - 1618-0631 (Electronic)
IS  - 0344-0338 (Linking)
VI  - 241
DP  - 2023 Jan
TI  - A preliminary analysis of prognostic genes in advanced laryngeal squamous cell 
      carcinoma patients with postoperative radiotherapy.
PG  - 154229
LID - S0344-0338(22)00473-3 [pii]
LID - 10.1016/j.prp.2022.154229 [doi]
AB  - Advanced laryngeal squamous cell carcinoma (LSCC) has a high mortality rate, and 
      the prognosis is poor. However, the underlying molecular biological mechanisms 
      bringing about the development and progression of advanced LSCC are not entirely 
      clarified. This study aimed to find out the potential biomarkers to predict the 
      prognosis in advanced LSCC patients who had undergone postoperative radiotherapy 
      alone. The next-generation sequencing of RNA was performed to detect the mRNAs 
      expression profiling in 10 advanced LSCC samples, comprised of 5 samples from 
      LSCC patients with favorable outcome and 5 samples from paired patients with poor 
      outcome. Then bioinformatics analysis including Gene Ontology (GO) and Kyoto 
      Encyclopedia of Genes and Genomes (KEGG) pathway were used to find out functional 
      core genes that were significantly different between the two groups. 1630 
      differentially expressed genes (DEGs) were confirmed to have significant 
      differences between the two groups. 53 GO terms and 19 pathways which were 
      closely related to the DEGs were identified. Finally, 52 intersection DEGs which 
      were both related to the top three GO terms and pathways were identified. The 
      expression of several core genes was confirmed with RT-qPCR in tissues from 
      another 75 patients. RT-qPCR confirmed that the genes of c-JUN, LYN, PIK3R2, and 
      TNFAIP3 were significantly differentially expressed between the two groups, which 
      was in accordance with the RNA sequencing data. The DEGs identified above may be 
      potential prognostic markers for advanced LSCC patients with postoperative 
      radiotherapy, and may provide essential guidance for following-up.
CI  - Copyright (c) 2022 Elsevier GmbH. All rights reserved.
FAU - Hu, Chunyan
AU  - Hu C
AD  - Department of Pathology, Eye & ENT Hospital, Fudan University, Shanghai 200031, 
      China.
FAU - Lao, Zheng
AU  - Lao Z
AD  - Department of Oral and Maxillofacial-Head & Neck Oncology, Ninth People's 
      Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 
      200011, China.
FAU - Yan, Li
AU  - Yan L
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, 83 
      Fenyang Rd., Shanghai 200031, China.
FAU - Song, Xinmao
AU  - Song X
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, 83 
      Fenyang Rd., Shanghai 200031, China. Electronic address: mhealthworker@163.com.
FAU - Wang, Jie
AU  - Wang J
AD  - Department of Radiation Oncology, Eye & ENT Hospital, Fudan University, 83 
      Fenyang Rd., Shanghai 200031, China. Electronic address: wangjie3955366@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221129
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Humans
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/radiotherapy
MH  - Prognosis
MH  - *Carcinoma, Squamous Cell/genetics/radiotherapy/metabolism
MH  - Gene Expression Profiling
MH  - *Laryngeal Neoplasms/genetics/radiotherapy/metabolism
MH  - Gene Regulatory Networks
MH  - *Head and Neck Neoplasms/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - Biomarkers, Tumor/genetics/metabolism
OTO - NOTNLM
OT  - Biomarker
OT  - Laryngeal squamous cell carcinoma
OT  - Postoperative radiotherapy
OT  - Prognosis
OT  - mRNA
COIS- Declaration of conflicting interest The authors declare that there are no 
      con fl icts of interest.
EDAT- 2022/12/13 06:00
MHDA- 2023/01/24 06:00
CRDT- 2022/12/12 18:27
PHST- 2022/08/06 00:00 [received]
PHST- 2022/11/12 00:00 [revised]
PHST- 2022/11/16 00:00 [accepted]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2023/01/24 06:00 [medline]
PHST- 2022/12/12 18:27 [entrez]
AID - S0344-0338(22)00473-3 [pii]
AID - 10.1016/j.prp.2022.154229 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2023 Jan;241:154229. doi: 10.1016/j.prp.2022.154229. Epub 2022 
      Nov 29.