PMID- 36509204
OWN - NLM
STAT- MEDLINE
DCOM- 20230105
LR  - 20230111
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 226
DP  - 2023 Jan 31
TI  - Multi-spectral and proteomic insights into the impact of proanthocyanidins on IgE 
      binding capacity and functionality in soy 11S protein during alkali-heating 
      treatment.
PG  - 597-607
LID - S0141-8130(22)02969-5 [pii]
LID - 10.1016/j.ijbiomac.2022.12.067 [doi]
AB  - This study evaluated the impact of proanthocyanidins on immunoglobulin E (IgE) 
      binding capacity, antioxidant, foaming and emulsifying properties in soy 11S 
      protein following alkali treatment at 80  degrees C for 20 min. The formation of >180 kDa 
      polymer was observed in the combined heating and proanthocyanidins-conjugation 
      treatment sample (11S-80PC) rather than in the heating treated sample (11S-80) 
      using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The 
      structural analyzes demonstrated that 11S-80PC exhibited more protein unfolding 
      than 11S-80. Heatmap analysis revealed that 11S-80PC had more alteration of 
      peptide and epitope profiles in 11S than in 11S-80. Molecular docking showed that 
      PC could well react with soy protein 11S. Liquid chromatography tandem MS 
      analysis (LC/MS-MS) demonstrated that there was a 35.6 % increase in 11S-80, but 
      a 14.5 % decrease in 11S-80PC for the abundance of total linear epitopes. As a 
      result, 11S-80PC exhibited more reduction in IgE binding capacities than 11S-80 
      owing to more obscuring and disruption of linear and conformational epitopes 
      induced by structural changes. Moreover, 11S-80PC exhibited higher antioxidant 
      capacities, foaming properties and emulsifying activity than 11S-80. Therefore, 
      the addition of proanthocyanidins could decrease allergenic activity and enhance 
      the functional properties of the heated soy 11S protein.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Pi, Xiaowen
AU  - Pi X
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Sun, Yuxue
AU  - Sun Y
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Key 
      Laboratory of Soybean Biology of Chinese Education Ministry, Harbin 150030, 
      China.
FAU - Liu, Jiafei
AU  - Liu J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Peng, Zeyu
AU  - Peng Z
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China.
FAU - Liang, Shuxia
AU  - Liang S
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China; Jiangsu 
      DAISY FSMP Co., Ltd, Nantong, Jiangsu 226133, China.
FAU - Cheng, Jianjun
AU  - Cheng J
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic 
      address: jjcheng@neau.edu.cn.
FAU - Jiang, Yunqing
AU  - Jiang Y
AD  - Northeast Agricultural University, Harbin, Heilongjiang 150030, China. Electronic 
      address: yqjiang188@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221209
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Soybean Proteins)
RN  - 0 (Proanthocyanidins)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 0 (Antioxidants)
RN  - 0 (Epitopes)
SB  - IM
MH  - *Soybean Proteins/chemistry
MH  - *Proanthocyanidins
MH  - Immunoglobulin E
MH  - Proteomics
MH  - Molecular Docking Simulation
MH  - Heating
MH  - Antioxidants
MH  - Epitopes/chemistry
OTO - NOTNLM
OT  - 11S
OT  - Conformational structure
OT  - Functional properties
OT  - IgE binding capacity
OT  - Proanthocyanidins
COIS- Declaration of competing interest All authors declare no conflict of interest.
EDAT- 2022/12/13 06:00
MHDA- 2023/01/06 06:00
CRDT- 2022/12/12 19:25
PHST- 2022/11/09 00:00 [received]
PHST- 2022/12/04 00:00 [revised]
PHST- 2022/12/07 00:00 [accepted]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2023/01/06 06:00 [medline]
PHST- 2022/12/12 19:25 [entrez]
AID - S0141-8130(22)02969-5 [pii]
AID - 10.1016/j.ijbiomac.2022.12.067 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Jan 31;226:597-607. doi: 
      10.1016/j.ijbiomac.2022.12.067. Epub 2022 Dec 9.