PMID- 36509413
OWN - NLM
STAT- MEDLINE
DCOM- 20230117
LR  - 20230117
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 132
DP  - 2023 Jan
TI  - Oral administration of Synechococcus sp. PCC7942 trans-vp19 and trans-vp (19+28) 
      genes improve the immune and antioxidant capacity in Procambarus clarkii under 
      white spot syndrome virus stress.
PG  - 108471
LID - S1050-4648(22)00804-X [pii]
LID - 10.1016/j.fsi.2022.108471 [doi]
AB  - This study aimed to evaluate antioxidant capacity and protection from white spot 
      syndrome virus (WSSV) challenge of Procambarus clarkii fed trans-vp19 and 
      trans-vp (19 + 28) genes of Synechococcus sp. PCC7942 (Syn7942). P. clarkii were 
      fed transgenic cyanobacteria continuously for 7 days, and then infected with WSSV 
      after 12 h starvation. The daily mortality in each group was measured for 10 days 
      and hepatopancreas and muscle of P. clarkii were examined for enzymes 
      phenoloxidase (PO) activity, catalase (CAT) activity, glutathione peroxidase 
      (GSH-px) activity, and malondialdehyde (MDA) concentration after immunization and 
      viral challenge at different times. Compared with the WSSV-infected crayfish in 
      positive control group (challenge and no vaccination) and wild type group 
      (challenge, feeding wild-type Syn7942), vp19 group (challenge, feeding Syn7942 
      trans-vp19 gene) and vp (19 + 28) group [challenge, feeding Syn7942 trans-vp 
      (19 + 28) genes] significantly improved the survival rate from 0% to 60% and 
      56.7%, respectively. Consistently, significantly greater PO, CAT, and GSH-px 
      activity and significantly lower MDA concentration in the vp19 and vp (19 + 28) 
      groups compared to the control group. These results demonstrate that the 
      trans-vp19 and trans-vp (19 + 28) gene of Syn7942 significantly facilitated the 
      immune and antioxidant capacity of crayfish. Therefore, the trans-vp19 and 
      trans-vp (19 + 28) genes of Syn7942 could provide protection for crayfish as an 
      anti-WSSV oral medication.
CI  - Copyright (c) 2022 Elsevier Ltd. All rights reserved.
FAU - Yang, Jia
AU  - Yang J
AD  - College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 
      201306, China.
FAU - Chi, Xiao-Ping
AU  - Chi XP
AD  - College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 
      201306, China.
FAU - Li, Man
AU  - Li M
AD  - Department of Pharmacy, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan 
      University, Shanghai, 201700, China.
FAU - He, Wen-Hui
AU  - He WH
AD  - College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 
      201306, China; Shanghai Engineering Research Center of River and Lake Biochain 
      Construction and Resource Utilization, Shanghai, 201702, China.
FAU - Jia, Rui
AU  - Jia R
AD  - College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 
      201306, China. Electronic address: rjia@shou.edu.cn.
FAU - He, Pei-Min
AU  - He PM
AD  - College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai, 
      201306, China. Electronic address: pmhe@shou.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20221209
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Antioxidants)
SB  - IM
MH  - Animals
MH  - Antioxidants
MH  - Astacoidea
MH  - *White spot syndrome virus 1/physiology
MH  - *Synechococcus/genetics
MH  - Administration, Oral
OTO - NOTNLM
OT  - Enzyme activities
OT  - Procambarus clarkii
OT  - Synechococcus sp. PCC7942
OT  - White spot syndrome virus (WSSV)
OT  - vp (19+28)
OT  - vp19
EDAT- 2022/12/13 06:00
MHDA- 2023/01/18 06:00
CRDT- 2022/12/12 19:29
PHST- 2022/11/07 00:00 [received]
PHST- 2022/11/27 00:00 [revised]
PHST- 2022/12/02 00:00 [accepted]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2023/01/18 06:00 [medline]
PHST- 2022/12/12 19:29 [entrez]
AID - S1050-4648(22)00804-X [pii]
AID - 10.1016/j.fsi.2022.108471 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2023 Jan;132:108471. doi: 10.1016/j.fsi.2022.108471. Epub 
      2022 Dec 9.