PMID- 36509520
OWN - NLM
STAT- MEDLINE
DCOM- 20221214
LR  - 20221221
IS  - 1002-0098 (Print)
IS  - 1002-0098 (Linking)
VI  - 57
IP  - 12
DP  - 2022 Dec 9
TI  - [Long non-coding RNA LINC01133 regulates cementogenic differentiation of human 
      periodontal ligament stem cells by modulating mitochondrial functions].
PG  - 1209-1216
LID - 10.3760/cma.j.cn112144-20220923-00501 [doi]
AB  - Objective: To investigate the effects of long non-coding RNA (lncRNA) LINC01133 
      on the cementogenic differentiation of human periodontal ligament stem cells 
      (hPDLSC) and the underlying mechanism. Methods: A total of 12 teeth were 
      harvested from 10 patients aged 17-30 years in the Department of Oral and 
      Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical 
      University for impacted or orthodontic reasons from September 2021 to January 
      2022. The hPDLSCs were isolated from the teeth and transfected with small 
      interfering RNA-LINC01133 (si-LINC01133) or small interfering RNA-negative 
      control (si-NC). The si-LINC01133 was regarded as the experimental group, and the 
      si-NC was regarded as the control one. The silencing efficiency of LINC01133 in 
      the hPDLSCs was evaluated by real-time quantitative PCR (RT-qPCR). Western 
      blotting was used to detect the protein expression levels of cementogenic 
      differentiation-related factors including bone sialoprotein (BSP), cementum 
      attachment protein (CAP), and cementum protein-1 (CEMP-1). Mitochondrial reactive 
      oxygen species (mtROS) production was assessed using the MitoSox by flow 
      cytometry. Mitochondrial membrane potential (MMP) was detected by JC-1 
      fluorescence staining. Mitochondrial respiratory chain complexes proteins 
      including NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8 (NDUFB8), 
      succinate dehydrogenase complex flavoprotein subunit A (SDHA), 
      ubiquinol-cytochrome c reductase core protein 1 (UQCR1), cytochrome c oxidase 
      subunit 4 isoform 1 (COXⅣ), and ATP synthase F1 subunit alpha (ATP5A) were 
      evaluated by Western blotting. Results: The expression levels of LINC01133 could 
      be suppressed by more than 60% with si-LINC01133 (control group: 1.000+/-0.000, 
      experimental group: 0.385+/-0.128) (t=10.72, P<0.01). Suppression of LINC01133 in 
      hPDLSCs decreased the levels of cementogenic differentiation-related proteins 
      including BSP (control group: 1.000+/-0.000, experimental group: 0.664+/-0.179) 
      (t=4.62, P<0.01) and CAP (control group: 1.000+/-0.000, experimental group: 
      0.736+/-0.229) (t=2.83, P<0.05). Suppression of LINC01133 in hPDLSCs increased the 
      production of mtROS (control group: 1.000+/-0.000, experimental group: 1.458+/-0.185) 
      (t=4.96, P<0.05) and the expression of NDUFB8 (control group: 1.000+/-0.000, 
      experimental group: 1.683+/-0.397) (t=3.45, P<0.05), as well as decreased MMP 
      levels (control group: 1.000+/-0.000, experimental group: 0.209+/-0.029) (t=53.99, 
      P<0.01) and the expression of SDHA (control group: 1.000+/-0.000, experimental 
      group: 0.428+/-0.228) (t=5.02, P<0.05). No significant changes in the UQCR1, COXⅣ, 
      and ATP5A expression levels were found between the control group and exprimental 
      group (P>0.05). Conclusions: LINC01133 regulates the cementogenic differentiation 
      of hPDLSCs possibly via modulating the mitochondrial functions.
FAU - Deng, D K
AU  - Deng DK
AD  - Department of Periodontology, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Engineering Research Center for 
      Dental Materials and Advanced Manufacture, Xi'an 710032, China.
FAU - Li, X
AU  - Li X
AD  - Department of Periodontology, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Engineering Research Center for 
      Dental Materials and Advanced Manufacture, Xi'an 710032, China.
FAU - He, X T
AU  - He XT
AD  - Department of Periodontology, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Engineering Research Center for 
      Dental Materials and Advanced Manufacture, Xi'an 710032, China.
FAU - Sun, H H
AU  - Sun HH
AD  - Department of General Dentistry & Emergency, School of Stomatology, The Fourth 
      Military Medical University & State Key Laboratory of Military Stomatology & 
      National Clinical Research Center for Oral Diseases & Shaanxi International Joint 
      Research Center For Oral Diseases, Xi'an 710032, China.
FAU - Tian, B M
AU  - Tian BM
AD  - Department of Periodontology, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Engineering Research Center for 
      Dental Materials and Advanced Manufacture, Xi'an 710032, China.
FAU - Chen, F M
AU  - Chen FM
AD  - Department of Periodontology, School of Stomatology, The Fourth Military Medical 
      University & State Key Laboratory of Military Stomatology & National Clinical 
      Research Center for Oral Diseases & Shaanxi Engineering Research Center for 
      Dental Materials and Advanced Manufacture, Xi'an 710032, China.
LA  - chi
GR  - 81970947, 82001102, 82170926, 82170958/National Natural Science Foundation of 
      China/
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Kou Qiang Yi Xue Za Zhi
JT  - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese 
      journal of stomatology
JID - 8711066
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Integrin-Binding Sialoprotein)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Humans
MH  - *Periodontal Ligament
MH  - *RNA, Long Noncoding/metabolism
MH  - Cells, Cultured
MH  - Stem Cells
MH  - Cell Differentiation
MH  - Integrin-Binding Sialoprotein/metabolism
MH  - Mitochondrial Proteins/metabolism
MH  - Mitochondria/genetics
MH  - RNA, Small Interfering/metabolism
MH  - Osteogenesis
EDAT- 2022/12/13 06:00
MHDA- 2022/12/15 06:00
CRDT- 2022/12/12 21:27
PHST- 2022/12/12 21:27 [entrez]
PHST- 2022/12/13 06:00 [pubmed]
PHST- 2022/12/15 06:00 [medline]
AID - 10.3760/cma.j.cn112144-20220923-00501 [doi]
PST - ppublish
SO  - Zhonghua Kou Qiang Yi Xue Za Zhi. 2022 Dec 9;57(12):1209-1216. doi: 
      10.3760/cma.j.cn112144-20220923-00501.