PMID- 36514009
OWN - NLM
STAT- MEDLINE
DCOM- 20221215
LR  - 20221230
IS  - 1528-3658 (Electronic)
IS  - 1076-1551 (Print)
IS  - 1076-1551 (Linking)
VI  - 28
IP  - 1
DP  - 2022 Dec 13
TI  - hUCMSCs carrying exenatide prevent T1DM by improving intestinal microflora 
      composition and islet tissue damage repair.
PG  - 155
LID - 10.1186/s10020-022-00526-0 [doi]
LID - 155
AB  - BACKGROUND: Exenatide is a stable analogue of glucagon-like peptide 1 that can 
      reduce postprandial hyperglycemia and has been utilized as adjunctive therapy for 
      type 1 diabetes mellitus (T1DM). The human umbilical cord is a rich source of 
      MSCs, and human umbilical cord mesenchymal stem cells (hUCMSCs) also show 
      potential to enhance insulin secretion. Here, we aimed to explore the effects of 
      hUCMSCs carrying exenatide in T1DM and further identify the possible mechanisms 
      involved. METHODS: hUCMSCs were isolated from human umbilical cord tissues, 
      identified, and transduced with recombinant lentivirus carrying exenatide to 
      obtain exenatide-carrying hUCMSCs (hUCMSCs@Ex-4). RESULTS: The results showed 
      that hUCMSCs@Ex-4 restored the blood glucose levels and body weight of NOD mice, 
      and repressed immune cell infiltration and islet tissue changes. Additionally, in 
      T1DM mice, treatment with hUCMSCs@Ex-4 reduced the blood glucose levels and 
      promoted repair of islet tissue damage. Moreover, hUCMSCs@Ex-4 attenuated renal 
      tissue lesions in T1DM mice. Applying bioinformatic analysis, the effects of 
      hUCMSCs@Ex-4 were suggested to correlate with decreased abundance of 
      pro-inflammatory intestinal bacteria and increased abundance of anti-inflammatory 
      intestinal bacteria. CONCLUSION: Overall, the study indicated that hUCMSCs 
      carrying exenatide might improve beneficial intestinal microflora abundance and 
      promote islet tissue damage repair, thereby alleviating T1DM.
CI  - (c) 2022. The Author(s).
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Hematology, The Affiliated Hospital of Qingdao University, Qingdao, 
      266000, People's Republic of China.
FAU - Wang, Yahao
AU  - Wang Y
AD  - Medical College, Qingdao University, Qingdao, 266071, People's Republic of China.
FAU - Chi, Jingwei
AU  - Chi J
AD  - Key Laboratory of Thyroid Diseases, Medical Research Center, The Affiliated 
      Hospital of Qingdao University, Qingdao, 266000, People's Republic of China.
FAU - Tan, Xiaojun
AU  - Tan X
AD  - Department of Endocrinology, Yidu Central Hospital of Weifang City, Weifang, 
      261000, People's Republic of China.
FAU - Hu, Jianxia
AU  - Hu J
AD  - The Laboratory of Thyroid Disease, The Affiliated Hospital of Qingdao University, 
      Qingdao, 266000, People's Republic of China.
FAU - Ma, Xiaolong
AU  - Ma X
AD  - Department of Endocrinology, Liaocheng People's Hospital, Liaocheng, 252000, 
      People's Republic of China.
FAU - Sun, Xiaofang
AU  - Sun X
AD  - Department of Endocrinology, The Affiliated Hospital of Qingdao University, No. 
      16, Jiangsu Road, South District, Qingdao, 266000, Shandong, People's Republic of 
      China.
FAU - Che, Kui
AU  - Che K
AD  - Key Laboratory of Thyroid Diseases, Medical Research Center, The Affiliated 
      Hospital of Qingdao University, Qingdao, 266000, People's Republic of China.
FAU - Lv, Wenshan
AU  - Lv W
AD  - Department of Endocrinology, The Affiliated Hospital of Qingdao University, No. 
      16, Jiangsu Road, South District, Qingdao, 266000, Shandong, People's Republic of 
      China.
FAU - Wang, Yangang
AU  - Wang Y
AUID- ORCID: 0000-0003-2103-7729
AD  - Department of Endocrinology, The Affiliated Hospital of Qingdao University, No. 
      16, Jiangsu Road, South District, Qingdao, 266000, Shandong, People's Republic of 
      China. wangyg@qdu.edu.
LA  - eng
PT  - Journal Article
DEP - 20221213
PL  - England
TA  - Mol Med
JT  - Molecular medicine (Cambridge, Mass.)
JID - 9501023
RN  - 9P1872D4OL (Exenatide)
RN  - 0 (Blood Glucose)
SB  - IM
MH  - Humans
MH  - Mice
MH  - Animals
MH  - Exenatide/pharmacology
MH  - *Diabetes Mellitus, Type 1
MH  - Blood Glucose
MH  - *Gastrointestinal Microbiome
MH  - Mice, Inbred NOD
MH  - *Mesenchymal Stem Cells
PMC - PMC9746121
OTO - NOTNLM
OT  - Exenatide
OT  - Human umbilical cord mesenchymal stem cells
OT  - Intestinal flora abundance
OT  - Islet tissue damage
OT  - Type 1 diabetes mellitus
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could be considered to influence the work reported in 
      this paper.
EDAT- 2022/12/14 06:00
MHDA- 2022/12/16 06:00
PMCR- 2022/12/13
CRDT- 2022/12/13 23:47
PHST- 2022/02/15 00:00 [received]
PHST- 2022/08/04 00:00 [accepted]
PHST- 2022/12/13 23:47 [entrez]
PHST- 2022/12/14 06:00 [pubmed]
PHST- 2022/12/16 06:00 [medline]
PHST- 2022/12/13 00:00 [pmc-release]
AID - 10.1186/s10020-022-00526-0 [pii]
AID - 526 [pii]
AID - 10.1186/s10020-022-00526-0 [doi]
PST - epublish
SO  - Mol Med. 2022 Dec 13;28(1):155. doi: 10.1186/s10020-022-00526-0.