PMID- 36514426
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231202
IS  - 2231-3796 (Print)
IS  - 0973-7707 (Electronic)
IS  - 2231-3796 (Linking)
VI  - 74
IP  - 4
DP  - 2022 Dec
TI  - Tumor Budding Assessment with Cytokeratin and Its Significance in Laryngeal 
      Squamous Cell Carcinomas.
PG  - 494-500
LID - 10.1007/s12070-021-02981-3 [doi]
AB  - There is a need for novel prognostic parameters in assessing laryngeal squamous 
      cell carcinoma (LSCC). Tumor budding is an instrumental parameter, which has 
      hardly been studied before in this organ. This study aimed to assess tumor 
      budding in LSCCs with routine hematoxylin and eosin (H&E) stain as well as 
      cytokeratin (CK) immunohistochemistry (IHC). Objectives were to compare the 
      effectiveness of both these methods to assess tumor budding, to investigate the 
      association of tumor budding and clinicopathologic features, and to determine the 
      prognostic significance of tumor budding in LSCCs. Fifty laryngectomy specimens 
      were included. Tumor budding was counted (20x) on slides stained with IHC-CK, and 
      highest count per slide was noted. The cases were classified as positive (> 1 
      buds) or negative (no buds present). The budding index was categorized as low 
      (< 5 buds) or high (> 5 buds). Tumor budding on H&E was absent, low and high 
      grade in 28%, 30% and 42% cases respectively, when compared to CK-IHC in 17%, 24% 
      and 59% of cases, respectively. Presence of lymphoplasmacytic infiltration 
      significantly correlated with tumor budding and higher grade. Transglottic 
      location of tumor and pT stage was associated with high budding. Presence of 
      lymphoplasmacytic infiltrate significantly correlated with worse prognosis. Tumor 
      budding, an easily assessable, inexpensive histopathologic parameter has seldom 
      been studied in LSCCs. Presence of lymphoplasmacytic infiltrate in routine 
      preoperative biopsy reporting could be useful in prognostication. CK-IHC is 
      helpful to detect especially cases with low-grade tumor budding.
CI  - (c) Association of Otolaryngologists of India 2021.
FAU - Gupta, Shreyanshi
AU  - Gupta S
AD  - Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, 
      Manipal, India. GRID: grid.411639.8. ISNI: 0000 0001 0571 5193
FAU - Sreeram, Saraswathy
AU  - Sreeram S
AUID- ORCID: 0000-0003-4149-0766
AD  - Department of Pathology, Kasturba Medical College, Mangalore, Manipal Academy of 
      Higher Education, Manipal, India. GRID: grid.411639.8. ISNI: 0000 0001 0571 5193
FAU - Pinto, Amanda Christina
AU  - Pinto AC
AD  - Department of Pathology, Kasturba Medical College, Mangalore, Manipal Academy of 
      Higher Education, Manipal, India. GRID: grid.411639.8. ISNI: 0000 0001 0571 5193
FAU - Suresh, Pooja Kundapur
AU  - Suresh PK
AD  - Department of Pathology, Kasturba Medical College, Mangalore, Manipal Academy of 
      Higher Education, Manipal, India. GRID: grid.411639.8. ISNI: 0000 0001 0571 5193
FAU - Basavaiah, Sridevi Hanaganahalli
AU  - Basavaiah SH
AD  - Department of Pathology, Kasturba Medical College, Mangalore, Manipal Academy of 
      Higher Education, Manipal, India. GRID: grid.411639.8. ISNI: 0000 0001 0571 5193
LA  - eng
PT  - Journal Article
DEP - 20211117
PL  - India
TA  - Indian J Otolaryngol Head Neck Surg
JT  - Indian journal of otolaryngology and head and neck surgery : official publication 
      of the Association of Otolaryngologists of India
JID - 9422551
PMC - PMC9741683
OTO - NOTNLM
OT  - Cytokeratin
OT  - Immunohistochemistry
OT  - Laryngeal carcinoma
OT  - Tumor budding
COIS- Conflict of interestThe authors have no relevant financial or non-financial 
      interests to disclose.
EDAT- 2022/12/15 06:00
MHDA- 2022/12/15 06:01
PMCR- 2023/12/01
CRDT- 2022/12/14 01:56
PHST- 2021/08/11 00:00 [received]
PHST- 2021/11/08 00:00 [accepted]
PHST- 2022/12/14 01:56 [entrez]
PHST- 2022/12/15 06:00 [pubmed]
PHST- 2022/12/15 06:01 [medline]
PHST- 2023/12/01 00:00 [pmc-release]
AID - 2981 [pii]
AID - 10.1007/s12070-021-02981-3 [doi]
PST - ppublish
SO  - Indian J Otolaryngol Head Neck Surg. 2022 Dec;74(4):494-500. doi: 
      10.1007/s12070-021-02981-3. Epub 2021 Nov 17.