PMID- 36514530
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221222
IS  - 1618-0240 (Print)
IS  - 1618-2863 (Electronic)
IS  - 1618-0240 (Linking)
VI  - 22
IP  - 12
DP  - 2022 Dec
TI  - Sporosarcina pasteurii can be used to print a layer of calcium carbonate.
PG  - 760-768
LID - 10.1002/elsc.202100074 [doi]
AB  - When using microbiologically induced calcium carbonate precipitation (MICP) to 
      produce calcium carbonate crystals in the cavities between mineral particles to 
      consolidate them, the inhomogeneous distribution of the precipitated calcium 
      carbonate poses a problem for the production of construction materials with 
      consistent parameters. Various approaches have been investigated in the 
      literature to increase the homogeneity of consolidated samples. One approach can 
      be the targeted application of ureolytic organisms by 3D printing. However, to 
      date, this possibility has been little explored in the literature. In this study, 
      the potential to use MICP to print calcium carbonate layers on mineral particles 
      will be investigated. For this purpose, a dispensing unit was modified to apply 
      both a suspension of Sporosarcina pasteurii and a calcination solution containing 
      urea and calcium chloride onto quartz sand. The study showed that after passing 
      through the nozzle, S. pasteurii preserved consistent cell vitality and therefore 
      its potential of MICP. Applying cell suspension and calcination solution through 
      a printing nozzle resulted in a layer of calcium carbonate crystals on quartz 
      sand. This observation demonstrated the proof of concept of printing calcium 
      carbonate by MICP through the nozzle of a dispensing unit. Furthermore, it was 
      shown that cell suspensions of S. pasteurii can be stored at 4 degrees C for a period of 
      17 days while maintaining its optical density, urease activity and cell vitality 
      and therefore the potential for MICP. This initial concept could be extended in 
      further research to printing three-dimensional (3D) objects to solve the problem 
      of homogeneity in consolidated mineral particles.
CI  - (c) 2022 The Authors. Engineering in Life Sciences published by Wiley-VCH GmbH.
FAU - Erdmann, Niklas
AU  - Erdmann N
AD  - Chair of Bioprocess Engineering Technical University of Kaiserslautern 
      Kaiserslautern Germany.
FAU - Kastner, Felix
AU  - Kastner F
AD  - Chair for Computional Physics in Engineering Technical University of 
      Kaiserslautern Kaiserslautern Germany.
FAU - de Payrebrune, Kristin
AU  - de Payrebrune K
AD  - Chair for Computional Physics in Engineering Technical University of 
      Kaiserslautern Kaiserslautern Germany.
FAU - Strieth, Dorina
AU  - Strieth D
AD  - Chair of Bioprocess Engineering Technical University of Kaiserslautern 
      Kaiserslautern Germany.
LA  - eng
PT  - Journal Article
DEP - 20220616
PL  - Germany
TA  - Eng Life Sci
JT  - Engineering in life sciences
JID - 101193313
PMC - PMC9731594
OTO - NOTNLM
OT  - 3D printing
OT  - Sporosarcina pasteurii
OT  - microbially induced calcium carbonate precipitation (MICP)
OT  - ureolytic activity
COIS- The authors have declared no conflicts of interest.
EDAT- 2022/12/15 06:00
MHDA- 2022/12/15 06:01
PMCR- 2022/06/16
CRDT- 2022/12/14 01:57
PHST- 2021/06/28 00:00 [received]
PHST- 2022/03/11 00:00 [revised]
PHST- 2022/05/15 00:00 [accepted]
PHST- 2022/12/14 01:57 [entrez]
PHST- 2022/12/15 06:00 [pubmed]
PHST- 2022/12/15 06:01 [medline]
PHST- 2022/06/16 00:00 [pmc-release]
AID - ELSC1529 [pii]
AID - 10.1002/elsc.202100074 [doi]
PST - epublish
SO  - Eng Life Sci. 2022 Jun 16;22(12):760-768. doi: 10.1002/elsc.202100074. 
      eCollection 2022 Dec.