PMID- 36515989
OWN - NLM
STAT- MEDLINE
DCOM- 20230213
LR  - 20240330
IS  - 2050-084X (Electronic)
IS  - 2050-084X (Linking)
VI  - 11
DP  - 2022 Dec 14
TI  - Gigapixel imaging with a novel multi-camera array microscope.
LID - 10.7554/eLife.74988 [doi]
LID - e74988
AB  - The dynamics of living organisms are organized across many spatial scales. 
      However, current cost-effective imaging systems can measure only a subset of 
      these scales at once. We have created a scalable multi-camera array microscope 
      (MCAM) that enables comprehensive high-resolution recording from multiple spatial 
      scales simultaneously, ranging from structures that approach the cellular scale 
      to large-group behavioral dynamics. By collecting data from up to 96 cameras, we 
      computationally generate gigapixel-scale images and movies with a field of view 
      over hundreds of square centimeters at an optical resolution of 18 microm. This 
      allows us to observe the behavior and fine anatomical features of numerous freely 
      moving model organisms on multiple spatial scales, including larval zebrafish, 
      fruit flies, nematodes, carpenter ants, and slime mold. Further, the MCAM 
      architecture allows stereoscopic tracking of the z-position of organisms using 
      the overlapping field of view from adjacent cameras. Overall, by removing the 
      bottlenecks imposed by single-camera image acquisition systems, the MCAM provides 
      a powerful platform for investigating detailed biological features and behavioral 
      processes of small model organisms across a wide range of spatial scales.
CI  - (c) 2022, Thomson et al.
FAU - Thomson, Eric E
AU  - Thomson EE
AUID- ORCID: 0000-0002-7118-2249
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
FAU - Harfouche, Mark
AU  - Harfouche M
AUID- ORCID: 0000-0002-4657-4603
AD  - Ramona Optics Inc, Durham, United States.
FAU - Kim, Kanghyun
AU  - Kim K
AUID- ORCID: 0000-0002-4557-9525
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Konda, Pavan C
AU  - Konda PC
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Seitz, Catherine W
AU  - Seitz CW
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
FAU - Cooke, Colin
AU  - Cooke C
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Xu, Shiqi
AU  - Xu S
AUID- ORCID: 0000-0002-8450-9001
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Jacobs, Whitney S
AU  - Jacobs WS
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
FAU - Blazing, Robin
AU  - Blazing R
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
FAU - Chen, Yang
AU  - Chen Y
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
FAU - Sharma, Sunanda
AU  - Sharma S
AD  - Ramona Optics Inc, Durham, United States.
FAU - Dunn, Timothy W
AU  - Dunn TW
AUID- ORCID: 0000-0002-9381-4630
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Park, Jaehee
AU  - Park J
AD  - Ramona Optics Inc, Durham, United States.
FAU - Horstmeyer, Roarke W
AU  - Horstmeyer RW
AUID- ORCID: 0000-0002-2480-9141
AD  - Ramona Optics Inc, Durham, United States.
AD  - Biomedical Engineering, Duke University, Durham, United States.
FAU - Naumann, Eva A
AU  - Naumann EA
AUID- ORCID: 0000-0002-7215-4717
AD  - Department of Neurobiology, Duke School of Medicine, Durham, United States.
LA  - eng
GR  - R43 EB030979/EB/NIBIB NIH HHS/United States
GR  - R43 OD024879/OD/NIH HHS/United States
GR  - R44 CA250877/CA/NCI NIH HHS/United States
GR  - R44 OD024879/OD/NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20221214
PL  - England
TA  - Elife
JT  - eLife
JID - 101579614
SB  - IM
MH  - Animals
MH  - *Zebrafish
MH  - *Microscopy/methods
PMC - PMC9917455
OTO - NOTNLM
OT  - C. elegans
OT  - Camponotus pennsylvanicus
OT  - D. melanogaster
OT  - Physarum polycephalum
OT  - behavioral analysis
OT  - calcium imaging
OT  - computational imaging
OT  - computational optics
OT  - microscopy
OT  - neuroscience
OT  - physics of living systems
OT  - zebrafish
COIS- ET, KK, PK, CS, CC, SX, WJ, RB, YC, TD, EN No competing interests declared, MH is 
      scientific co-founder at Ramona Optics Inc which is commercializing and patenting 
      the multi-camera array microscope, SS, JP was an employee at Ramona Optics Inc, 
      which is commercializing and patenting the multi-camera array microscope, RH is a 
      scientific co-founder at Ramona Optics Inc, which is commercializing and 
      patenting the multi-camera array microscope
EDAT- 2022/12/15 06:00
MHDA- 2023/02/14 06:00
PMCR- 2022/12/14
CRDT- 2022/12/14 11:53
PHST- 2021/10/25 00:00 [received]
PHST- 2022/11/23 00:00 [accepted]
PHST- 2022/12/15 06:00 [pubmed]
PHST- 2023/02/14 06:00 [medline]
PHST- 2022/12/14 11:53 [entrez]
PHST- 2022/12/14 00:00 [pmc-release]
AID - 74988 [pii]
AID - 10.7554/eLife.74988 [doi]
PST - epublish
SO  - Elife. 2022 Dec 14;11:e74988. doi: 10.7554/eLife.74988.