PMID- 36516653
OWN - NLM
STAT- MEDLINE
DCOM- 20230203
LR  - 20230213
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 383
DP  - 2023 Jan
TI  - Diverse and divergent functions of IL-32beta and IL-32gamma isoforms in the regulation 
      of malignant pleural mesothelioma cell growth and the production of VEGF-A and 
      CXCL8.
PG  - 104652
LID - S0008-8749(22)00177-0 [pii]
LID - 10.1016/j.cellimm.2022.104652 [doi]
AB  - In this study, we sought to elucidate the roles of the interleukin (IL)-32beta and 
      IL-32gamma in mesothelioma cell growth, and vascular endothelial growth factor 
      (VEGF)-A and C-X-C motif chemokine ligand 8 (CXCL8) expression. IL-32 elicited a 
      growth-promoting effect against one of the six mesotheliomas lines and exerted 
      diverse regulatory functions in VEGF-A and CXCL8 secretion from mesotheliomas 
      stimulated with or without IL-17A. Retroviral-mediated transduction of 
      mesothelioma lines with IL-32gamma resulted in enhanced IL-32beta expression, which 
      facilitated or suppressed the in vitro growth, and VEGF-A and CXCL8 expression. 
      Overexpressed IL-32beta-augmented growth and VEGF-A and CXCL8 production were mainly 
      mediated through the phosphatidylinositol-3 kinase (PI3K) signaling pathway. On 
      the other hand, overexpressed IL-32beta-deceased growth was mediated through 
      mitogen-activated protein kinase (MAPK) pathway. NCI-H2373IL-32gamma tumors grew 
      faster than NCI-H2373Neo tumors in a xenograft model, which was associated with 
      increased vascularity. These findings indicate that IL-32 are involved in the 
      regulation of growth and angiogenic factor production in mesotheliomas.
CI  - Copyright (c) 2022 Elsevier Inc. All rights reserved.
FAU - Numasaki, Muneo
AU  - Numasaki M
AD  - Laboratory of Clinical Science and Biomedicine, Faculty of Pharmaceutical 
      Sciences, Josai University, Sakado, Saitama, Japan; Department of Geriatrics and 
      Gerontology, Institute of Development, Aging and Cancer, Tohoku University, 
      Sendai, Miyagi, Japan; Department of Nursing, Faculty of Medical Science and 
      Welfare, Tohoku Bunka Gakuen University, Sendai, Miyagi, Japan; Laboratory of 
      Clinical Science and Biomedicine, Faculty of Pharmaceutical Sciences, Josai 
      University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan. Electronic address: 
      mnumasaki@ns.tbgu.ac.jp.
FAU - Ito, Koyu
AU  - Ito K
AD  - Department of Immunology, Institute of Development, Aging and Cancer, Tohoku 
      University, Sendai, Miyagi, Japan.
FAU - Takagi, Kiyoshi
AU  - Takagi K
AD  - Department of Pathology and Histotechnology, Tohoku University Graduate School of 
      Medicine, Sendai, Miyagi, Japan.
FAU - Nagashima, Kengo
AU  - Nagashima K
AD  - Biostatistics Unit, Clinical and Translational Research Center, Keio University 
      Hospital, Tokyo, Tokyo, Japan.
FAU - Notsuda, Hirotsugu
AU  - Notsuda H
AD  - Department of Thoracic Surgery, Institute of Development, Aging and Cancer, 
      Tohoku University, Sendai, Miyagi, Japan.
FAU - Ogino, Hirokazu
AU  - Ogino H
AD  - Department of Respiratory Medicine and Rheumatology, Graduate School of 
      Biomedical Sciences, Tokushima University, Tokushima, Tokushima, Japan.
FAU - Ando, Rika
AU  - Ando R
AD  - Department of Nursing, Faculty of Medical Science and Welfare, Tohoku Bunka 
      Gakuen University, Sendai, Miyagi, Japan.
FAU - Tomioka, Yoshihisa
AU  - Tomioka Y
AD  - Laboratory of Oncology, Pharmacy Practice and Sciences, Graduate School of 
      Pharmaceutical Sciences, Tohoku University, Sendai, Miyagi, Japan.
FAU - Suzuki, Takashi
AU  - Suzuki T
AD  - Department of Pathology and Histotechnology, Tohoku University Graduate School of 
      Medicine, Sendai, Miyagi, Japan.
FAU - Okada, Yoshinori
AU  - Okada Y
AD  - Department of Thoracic Surgery, Institute of Development, Aging and Cancer, 
      Tohoku University, Sendai, Miyagi, Japan.
FAU - Nishioka, Yasuhiko
AU  - Nishioka Y
AD  - Department of Respiratory Medicine and Rheumatology, Graduate School of 
      Biomedical Sciences, Tokushima University, Tokushima, Tokushima, Japan.
FAU - Unno, Michiaki
AU  - Unno M
AD  - Department of Surgery, Tohoku University Graduate School of Medicine, Sendai, 
      Miyagi, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221130
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Interleukins)
RN  - 0 (Protein Isoforms)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (IL32G protein, human)
RN  - 0 (IL32 protein, human)
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Interleukin-8)
SB  - IM
MH  - Humans
MH  - *Interleukins/genetics/metabolism
MH  - *Mesothelioma, Malignant/metabolism/pathology
MH  - Protein Isoforms/metabolism
MH  - *Vascular Endothelial Growth Factor A/genetics/metabolism
MH  - *Interleukin-8/metabolism
OTO - NOTNLM
OT  - CXCL8
OT  - IL-17A
OT  - IL-32
OT  - Malignant pleural mesothelioma
OT  - VEGF-A
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2022/12/15 06:00
MHDA- 2023/01/11 06:00
CRDT- 2022/12/14 18:27
PHST- 2022/05/29 00:00 [received]
PHST- 2022/11/23 00:00 [revised]
PHST- 2022/11/27 00:00 [accepted]
PHST- 2022/12/15 06:00 [pubmed]
PHST- 2023/01/11 06:00 [medline]
PHST- 2022/12/14 18:27 [entrez]
AID - S0008-8749(22)00177-0 [pii]
AID - 10.1016/j.cellimm.2022.104652 [doi]
PST - ppublish
SO  - Cell Immunol. 2023 Jan;383:104652. doi: 10.1016/j.cellimm.2022.104652. Epub 2022 
      Nov 30.