PMID- 36518433
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221222
IS  - 2214-7500 (Electronic)
IS  - 2214-7500 (Linking)
VI  - 9
DP  - 2022
TI  - Lack of skin sensitization hazard potential for alpha-glycosyl isoquercitrin 
      (AGIQ) utilizing the Local Lymph Node Assay.
PG  - 1291-1296
LID - 10.1016/j.toxrep.2022.05.021 [doi]
AB  - Skin sensitization is an important aspect of safety assessment and is a key 
      component in the toxicological evaluation of chemicals. alpha-Glycosyl 
      isoquercitrin (AGIQ), is marketed in Japan as a food additive and is generally 
      recognized as safe (GRAS) by the expert panel of the Flavor and Extract 
      Manufacturers Association (FEMA) in 2005 and the U.S. Food and Drug 
      Administration (FDA) in 2007. The Local Lymph Node Assay (LLNA) was used to 
      assess AGIQ's potential to cause skin sensitization. Results indicate that no 
      excessive irritation was observed after the irritation screen (ear swelling 
      < 25 % and erythema score < 3) when AGIQ was tested at 5 %, 10 %, and 25 % in N, 
      N-dimethyl formamide [DMF]. Based on lack of irritation, AGIQ was further 
      evaluated at 10 %, 25 %, and 50 % in DMF in the main test resulting in 
      stimulation indices of less than the positive threshold of 1.6 i.e., 1.2, 1.4, 
      and 1.2 respectively. Therefore, AGIQ was not a dermal sensitizer in the LLNA.
CI  - (c) 2022 The Authors.
FAU - Vij, Puneet
AU  - Vij P
AD  - MB Research Laboratory, 1765 Wentz Road, Spinnerstown, PA 18968, USA.
FAU - Donahue, Douglas A
AU  - Donahue DA
AD  - Integrated Laboratory Systems, Inc., PO Box 13501, Research Triangle Park, NC 
      27709, USA.
FAU - Burke, Keith P
AU  - Burke KP
AD  - Integrated Laboratory Systems, Inc., PO Box 13501, Research Triangle Park, NC 
      27709, USA.
FAU - Hayashi, Shim-Mo
AU  - Hayashi SM
AD  - Division of Food Additives, National Institute of Health Sciences, 3-25-26 
      Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-9501, Japan.
FAU - Maronpot, Robert R
AU  - Maronpot RR
AD  - Maronpot Consulting LLC, 1612 Medfield Road, Raleigh, NC 27607, USA.
LA  - eng
PT  - Journal Article
DEP - 20220603
PL  - Ireland
TA  - Toxicol Rep
JT  - Toxicology reports
JID - 101630272
PMC - PMC9742868
OTO - NOTNLM
OT  - AGIQ
OT  - Alpha-glycosyl isoquercitrin
OT  - CBA/J mice
OT  - Irritation
OT  - Isoquercitrin
OT  - LLNA
OT  - Local Lymph Node Assay
OT  - OECD 442B
OT  - Safety
OT  - Sensitization
OT  - Stimulation index (ices)
OT  - Toxicity
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2022/12/16 06:00
MHDA- 2022/12/16 06:01
PMCR- 2022/06/03
CRDT- 2022/12/15 02:21
PHST- 2022/04/25 00:00 [received]
PHST- 2022/05/27 00:00 [revised]
PHST- 2022/05/30 00:00 [accepted]
PHST- 2022/12/15 02:21 [entrez]
PHST- 2022/12/16 06:00 [pubmed]
PHST- 2022/12/16 06:01 [medline]
PHST- 2022/06/03 00:00 [pmc-release]
AID - S2214-7500(22)00136-6 [pii]
AID - 10.1016/j.toxrep.2022.05.021 [doi]
PST - epublish
SO  - Toxicol Rep. 2022 Jun 3;9:1291-1296. doi: 10.1016/j.toxrep.2022.05.021. 
      eCollection 2022.