PMID- 36518899
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221221
IS  - 2297-1769 (Print)
IS  - 2297-1769 (Electronic)
IS  - 2297-1769 (Linking)
VI  - 9
DP  - 2022
TI  - Expression of matrix metalloproteinases (MMPs)-2/-7/-9/-14 and tissue inhibitors 
      of MMPs (TIMPs)-1/-2 in bovine cutaneous fibropapillomas associated with BPV-2 
      infection.
PG  - 1063580
LID - 10.3389/fvets.2022.1063580 [doi]
LID - 1063580
AB  - INTRODUCTION: Bovine papillomaviruses -1/-2 (BPVs) are small non-enveloped 
      double-stranded DNA viruses able to infect the skin of bovids and equids, causing 
      development of neoplastic lesions such as bovine cutaneous fibropapillomas and 
      equine sarcoid. Matrix metalloproteinases (MMPs) are a group of zinc-dependent 
      endopeptidases that degrade basal membrane and extracellular matrix, whose 
      function is essential in physiological processes such as tissue remodeling and 
      wound healing. MMPs activity is finely regulated by a balancing with expression 
      of tissue inhibitors of MMPs (TIMPs), a process that is impaired during tumour 
      development. BPV infection is associated with upregulation of MMPs and /or their 
      unbalancing with TIMPs, contributing to local invasion and impairment of 
      extracellular matrix remodeling in equine sarcoid; however, studies regarding 
      this topic in bovine fibropapillomas are lacking. METHODS: The aim of this study 
      was to perform an immunohistochemical and biochemical analysis on a panel of MMPs 
      and TIMPs in BPV-2 positive bovine cutaneous fibropapillomas vs. normal skin 
      samples. RESULTS: Immunohistochemistry revealed a cytoplasmic expression of MMP-2 
      (15/19), a cytoplasmic and perinuclear immunoreactivity of MMP-7 (19/19) and 
      MMP-9 (19/19), along with a cytoplasmic and nuclear pattern of MMP-14 (16/19), 
      accompanied by a cytoplasmic expression of TIMP-1 (14/19) and TIMP-2 (18/19) in 
      tumour samples; western blotting revealed an overexpression of MMP-2 (8/9), MMP-7 
      (9/9) and MMP-9 (9/9), and a decreased level of MMP-14 (9/9), TIMP-1 (9/9) and 
      TIMP-2 (9/9) in tumour versus normal skin samples. Moreover, gelatine zymography 
      confirmed the expression of pro-active MMP-2 (9/9) and MMP-9 (9/9) and, most 
      importantly, indicated the presence and increased activity of their active forms 
      (82 and 62 kDa, respectively) in tumour samples. DISCUSSION: This is the first 
      study describing MMPs and TIMPs in bovine cutaneous fibropapillomas and our 
      results suggest that their unbalanced expression in presence of BPV-2 may play a 
      significant role in tumour development. A further analysis of supplementary MMPs 
      and TIMPs could bring new important insights into the papillomavirus induced 
      tumours.
CI  - Copyright (c) 2022 Daraban Bocaneti, Altamura, Corteggio, Tanase, Dascalu, Pasca, 
      Hritcu, Mares and Borzacchiello.
FAU - Daraban Bocaneti, Florentina
AU  - Daraban Bocaneti F
AD  - Department of Public Health, Faculty of Veterinary Medicine, Iasi University of 
      Life Sciences "Ion Ionescu de la Brad", Iasi, Romania.
FAU - Altamura, Gennaro
AU  - Altamura G
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Naples, Italy.
FAU - Corteggio, Annunziata
AU  - Corteggio A
AD  - Institute of Biochemistry and Cell Biology (IBBC), National Research Council 
      (CNR), Naples, Italy.
FAU - Tanase, Oana Irina
AU  - Tanase OI
AD  - Department of Public Health, Faculty of Veterinary Medicine, Iasi University of 
      Life Sciences "Ion Ionescu de la Brad", Iasi, Romania.
FAU - Dascalu, Mihaela Anca
AU  - Dascalu MA
AD  - Department of Public Health, Faculty of Veterinary Medicine, Iasi University of 
      Life Sciences "Ion Ionescu de la Brad", Iasi, Romania.
FAU - Pasca, Sorin Aurelian
AU  - Pasca SA
AD  - Department of Pathology, Faculty of Veterinary Medicine, Iasi University of Life 
      Sciences "Ion Ionescu de la Brad", Iasi, Romania.
FAU - Hritcu, Ozana
AU  - Hritcu O
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Naples, Italy.
FAU - Mares, Mihai
AU  - Mares M
AD  - Department of Public Health, Faculty of Veterinary Medicine, Iasi University of 
      Life Sciences "Ion Ionescu de la Brad", Iasi, Romania.
FAU - Borzacchiello, Giuseppe
AU  - Borzacchiello G
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20221128
PL  - Switzerland
TA  - Front Vet Sci
JT  - Frontiers in veterinary science
JID - 101666658
PMC - PMC9744227
OTO - NOTNLM
OT  - BPV
OT  - MMP
OT  - TIMP
OT  - bovine fibropapilloma
OT  - tumor invasion
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2022/12/16 06:00
MHDA- 2022/12/16 06:01
PMCR- 2022/01/01
CRDT- 2022/12/15 02:32
PHST- 2022/10/07 00:00 [received]
PHST- 2022/11/04 00:00 [accepted]
PHST- 2022/12/15 02:32 [entrez]
PHST- 2022/12/16 06:00 [pubmed]
PHST- 2022/12/16 06:01 [medline]
PHST- 2022/01/01 00:00 [pmc-release]
AID - 10.3389/fvets.2022.1063580 [doi]
PST - epublish
SO  - Front Vet Sci. 2022 Nov 28;9:1063580. doi: 10.3389/fvets.2022.1063580. 
      eCollection 2022.