PMID- 36519006
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221221
IS  - 2573-2102 (Electronic)
IS  - 2573-2102 (Linking)
VI  - 6
IP  - 4
DP  - 2022 Oct
TI  - Evaluating dry vs. wet disinfection in boot baths on detection of porcine 
      epidemic diarrhea virus and porcine reproductive and respiratory virus RNA.
PG  - txac150
LID - 10.1093/tas/txac150 [doi]
LID - txac150
AB  - Maintaining biosecurity between swine barns is challenging, and boot baths are an 
      easily implementable option some utilize to limit pathogen spread. However, there 
      are concerns regarding their efficacy, especially when comparing wet or dry 
      disinfectants. The objective of this study was to evaluate the efficacy of boot 
      baths in reducing the quantity of detectable porcine epidemic diarrhea virus 
      (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) genetic 
      material using wet or dry disinfectants. Treatments included 1) control, 2) dry 
      chlorine powder (Traffic C.O.P., PSP, LLC, Rainsville, AL), and 3) wet quaternary 
      ammonium/glutaraldehyde liquid (1:256 Synergize, Neogen, Lexington, KY). Prior to 
      disinfection, rubber boots were inoculated with 1 mL of a co-inoculants of PRRSV 
      (1 x 10(5) TCID(50) per mL) and PEDV (1 x 10(5) TCID(50) per mL) and dried for 15 
      min. After the drying period, a researcher placed the boot on the right foot and 
      stepped directly on a stainless steel coupon (control). Alternatively, the 
      researcher stepped first into a boot bath containing either the wet or dry 
      sanitizer, stood for 3 s, and then stepped onto a steel coupon. After one minute, 
      an environmental swab was then collected and processed from each boot and steel 
      coupon. The procedure was replicated 12 times per disinfectant treatment. Samples 
      were analyzed using a duplex qPCR at the Kansas State Veterinary Diagnostic 
      Laboratory. Cycle threshold values were analyzed using SAS GLIMMIX v 9.4 (SAS, 
      Inc., Cary, NC). There was no evidence of a disinfectant x surface x virus 
      interaction (P > 0.10). An interaction between disinfectant x surface impacted (P 
      < 0.05) the quantity of detectable viral RNA. As expected, the quantity of the 
      viruses on the coupon was greatest in the control, indicating that a contaminated 
      boot has the ability to transfer viruses from a contaminated surface to a clean 
      surface. Comparatively, the dry disinfectant treatment resulted in no detectable 
      viral RNA on either the boot or subsequent coupon. The wet disinfectant treatment 
      had statistically similar (P > 0.05) viral contamination to the control on the 
      boot, but less viral contamination compared to the control on the metal coupon. 
      In this experiment, a boot bath with dry powder was the most efficacious in 
      reducing the detectable viral RNA on both boots and subsequent surfaces.
CI  - (c) The Author(s) 2022. Published by Oxford University Press on behalf of the 
      American Society of Animal Science.
FAU - Harrison, Olivia L
AU  - Harrison OL
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan, 
      KS, USA.
FAU - Houston, Grace E
AU  - Houston GE
AD  - Department of Diagnostic Medicine/Pathobiology, Kansas State University, 
      Manhattan, KS, USA.
FAU - Blomme, Allison K
AU  - Blomme AK
AD  - Department of Grain Science and Industry, Kansas State University, Manhattan, KS, 
      USA.
FAU - Otott, Haley K
AU  - Otott HK
AD  - Department of Grain Science and Industry, Kansas State University, Manhattan, KS, 
      USA.
FAU - Bai, Jianfa
AU  - Bai J
AD  - Department of Diagnostic Medicine/Pathobiology, Kansas State University, 
      Manhattan, KS, USA.
FAU - Poulsen Porter, Elizabeth G
AU  - Poulsen Porter EG
AD  - Department of Diagnostic Medicine/Pathobiology, Kansas State University, 
      Manhattan, KS, USA.
FAU - Woodworth, Jason C
AU  - Woodworth JC
AUID- ORCID: 0000-0001-7268-4278
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan, 
      KS, USA.
FAU - Paulk, Chad B
AU  - Paulk CB
AD  - Department of Grain Science and Industry, Kansas State University, Manhattan, KS, 
      USA.
FAU - Gebhardt, Jordan T
AU  - Gebhardt JT
AUID- ORCID: 0000-0002-6144-6714
AD  - Department of Diagnostic Medicine/Pathobiology, Kansas State University, 
      Manhattan, KS, USA.
FAU - Jones, Cassandra K
AU  - Jones CK
AUID- ORCID: 0000-0002-0671-8879
AD  - Department of Animal Sciences and Industry, Kansas State University, Manhattan, 
      KS, USA.
LA  - eng
PT  - Journal Article
DEP - 20221112
PL  - England
TA  - Transl Anim Sci
JT  - Translational animal science
JID - 101738705
PMC - PMC9744243
OTO - NOTNLM
OT  - PEDV
OT  - PRRSV
OT  - boot bath
OT  - swine
EDAT- 2022/12/16 06:00
MHDA- 2022/12/16 06:01
PMCR- 2022/11/12
CRDT- 2022/12/15 02:35
PHST- 2022/08/24 00:00 [received]
PHST- 2022/11/08 00:00 [accepted]
PHST- 2022/12/15 02:35 [entrez]
PHST- 2022/12/16 06:00 [pubmed]
PHST- 2022/12/16 06:01 [medline]
PHST- 2022/11/12 00:00 [pmc-release]
AID - txac150 [pii]
AID - 10.1093/tas/txac150 [doi]
PST - epublish
SO  - Transl Anim Sci. 2022 Nov 12;6(4):txac150. doi: 10.1093/tas/txac150. eCollection 
      2022 Oct.