PMID- 36519852 OWN - NLM STAT- MEDLINE DCOM- 20230216 LR - 20231020 IS - 2165-0497 (Electronic) IS - 2165-0497 (Linking) VI - 11 IP - 1 DP - 2023 Feb 14 TI - Multicenter Evaluation of the FilmArray Blood Culture Identification 2 Panel for Pathogen Detection in Bloodstream Infections. PG - e0254722 LID - 10.1128/spectrum.02547-22 [doi] LID - e02547-22 AB - The FilmArray Blood Culture Identification 2 panel (BCID2; bioMerieux) is a fully automated PCR-based assay for identifying bacteria, fungi, and bacterial resistance markers in positive blood cultures (BC) in about 1 h. In this multicenter study, we evaluated the performance of the BCID2 panel for pathogen detection in positive BC. Conventional culture and BCID2 were performed in parallel at four tertiary-care hospitals. We included 152 positive BC-130 monomicrobial and 22 polymicrobial cultures-in this analysis. The BCID2 assay correctly identified 90% (88/98) of Gram-negative and 89% (70/79) of Gram-positive bacteria. Five bacterial isolates targeted by the BCID2 panel and recovered from five positive BC, including three polymicrobial cultures, were missed by the BCID2 assay. Fifteen isolates were off-panel organisms, accounting for 8% (15/182) of the isolates obtained from BC. The mean positive percent agreement between the BCID2 assay and standard culture was 97% (95% confidence interval, 95 to 99%), with agreement ranging from 67% for Candida albicans to 100% for 17 targets included in the BCID2 panel. BCID2 also identified the bla(CTX-M) gene in seven BC, including one for which no extended-spectrum beta-lactamase (ESBL)-producing isolate was obtained in culture. However, it failed to detect ESBL-encoding genes in three BC. Two of the 18 mecA/C genes detected by the BCID2 were not confirmed. No carbapenemase, mecA/C, or MREJ targets were detected. The median turnaround time was significantly shorter for BCID2 than for culture. The BCID2 panel may facilitate faster pathogen identification in bloodstream infections. IMPORTANCE Rapid molecular diagnosis combining the identification of pathogens and the detection of antibiotic resistance genes from positive blood cultures (BC) can improve the outcome for patients with bloodstream infections. The FilmArray BCID2 panel, an updated version of the original BCID, can detect 11 Gram-positive bacteria, 15 Gram-negative bacteria, 7 fungal pathogens, and 10 antimicrobial resistance genes directly from a positive BC. Here, we evaluated the real-life microbiological performance of the BCID2 assay in comparison to the results of standard methods used in routine practice at four tertiary care hospitals. FAU - Camelena, Francois AU - Camelena F AUID- ORCID: 0000-0002-0958-4802 AD - Departement de Bacteriologie, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. AD - Universite Paris Cite and Universite Sorbonne Paris Nord, INSERM, IAME, Paris, France. FAU - Pean de Ponfilly, Gauthier AU - Pean de Ponfilly G AD - Service de Microbiologie clinique, Groupe hospitalier Paris Saint Joseph, Paris, France. AD - Institut Micalis UMR 1319, Universite Paris-Saclay, INRAe, AgroParisTech, Chatenay Malabry, France. FAU - Pailhories, Helene AU - Pailhories H AD - Laboratoire de Bacteriologie, Institut de Biologie en Sante, Centre Hospitalier Universitaire d'Angers, Laboratoire HIFIH, UPRES EA3859, SFR ICAT 4208, Universite d'Angers, Angers, France. FAU - Bonzon, Lucas AU - Bonzon L AD - Laboratoire de Bacteriologie, Centre Hospitalier Universitaire de Montpellier et MIVEGEC, UMR IRD-CNRS-Universite de Montpellier, Montpellier, France. FAU - Alanio, Alexandre AU - Alanio A AD - Laboratoire de Parasitologie-Mycologie, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. AD - Institut Pasteur, Universite Paris Cite, CNRS, Unite de Mycologie Moleculaire, UMR2000, Paris, France. FAU - Poncin, Thibaut AU - Poncin T AD - Departement de Bacteriologie, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. AD - Universite Paris Cite and Universite Sorbonne Paris Nord, INSERM, IAME, Paris, France. FAU - Lafaurie, Matthieu AU - Lafaurie M AD - Departement des Maladies Infectieuses et Tropicales, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. FAU - Depret, Francois AU - Depret F AD - Departement d'Anesthesie et Reanimation chirurgicale et brules, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. FAU - Cambau, Emmanuel AU - Cambau E AD - Universite Paris Cite and Universite Sorbonne Paris Nord, INSERM, IAME, Paris, France. AD - Laboratoire de mycobacteriologie specialisee et de reference, Laboratoire associe du Centre National de Reference des mycobacteries et resistance des mycobacteries aux antituberculeux, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. FAU - Godreuil, Sylvain AU - Godreuil S AD - Laboratoire de Bacteriologie, Centre Hospitalier Universitaire de Montpellier et MIVEGEC, UMR IRD-CNRS-Universite de Montpellier, Montpellier, France. FAU - Chenouard, Rachel AU - Chenouard R AD - Laboratoire de Bacteriologie, Institut de Biologie en Sante, Centre Hospitalier Universitaire d'Angers, Laboratoire HIFIH, UPRES EA3859, SFR ICAT 4208, Universite d'Angers, Angers, France. FAU - Le Monnier, Alban AU - Le Monnier A AD - Service de Microbiologie clinique, Groupe hospitalier Paris Saint Joseph, Paris, France. AD - Institut Micalis UMR 1319, Universite Paris-Saclay, INRAe, AgroParisTech, Chatenay Malabry, France. FAU - Jacquier, Herve AU - Jacquier H AD - Departement de Bacteriologie, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. AD - Universite Paris Cite and Universite Sorbonne Paris Nord, INSERM, IAME, Paris, France. FAU - Bercot, Beatrice AU - Bercot B AUID- ORCID: 0000-0003-1603-3635 AD - Departement de Bacteriologie, Groupe Hospitalier Saint-Louis-Lariboisiere-Fernand Widal, Assistance Publique-Hopitaux de Paris, Paris, France. AD - Universite Paris Cite and Universite Sorbonne Paris Nord, INSERM, IAME, Paris, France. LA - eng PT - Journal Article PT - Multicenter Study PT - Research Support, Non-U.S. Gov't DEP - 20221215 PL - United States TA - Microbiol Spectr JT - Microbiology spectrum JID - 101634614 SB - IM MH - Humans MH - Blood Culture MH - *Sepsis/diagnosis MH - Bacteria/genetics MH - Gram-Negative Bacteria/genetics MH - Gram-Positive Bacteria MH - *Bacteremia/diagnosis/microbiology PMC - PMC9927563 OTO - NOTNLM OT - BCID2 OT - BSI OT - Biofire OT - FilmArray OT - bloodstream infection OT - genotypic identification OT - molecular diagnosis OT - rapid diagnosis OT - resistance gene OT - sepsis COIS- The authors declare a conflict of interest. This study was supported by bioMerieux (Marcy L'Etoile, France), which provided consumables. The funders had no role in study design, data collection and interpretation, or manuscript preparation. F.C. received lecture fees from bioMerieux. F.D. received lecture fees from bioMerieux, Sedana and grants from European Society of Intensive Care medicine, Societe Francaise d'Anesthesie Reanimation and from the French ministry of research. A.A. received lecture fees and travel grants from Gilead science and Pfizer. He received grants from Agence Nationale de la Recherche. Other authors report no conflicts of interest. EDAT- 2022/12/16 06:00 MHDA- 2023/02/17 06:00 PMCR- 2022/12/15 CRDT- 2022/12/15 09:03 PHST- 2022/12/16 06:00 [pubmed] PHST- 2023/02/17 06:00 [medline] PHST- 2022/12/15 09:03 [entrez] PHST- 2022/12/15 00:00 [pmc-release] AID - 02547-22 [pii] AID - spectrum.02547-22 [pii] AID - 10.1128/spectrum.02547-22 [doi] PST - ppublish SO - Microbiol Spectr. 2023 Feb 14;11(1):e0254722. doi: 10.1128/spectrum.02547-22. Epub 2022 Dec 15.