PMID- 36523286
OWN - NLM
STAT- MEDLINE
DCOM- 20221219
LR  - 20221222
IS  - 1937-8688 (Electronic)
VI  - 43
DP  - 2022
TI  - Association between haematological values and heat shock protein 70 of sickle 
      cell disease patients in Ado-Ekiti, Ekiti State, Nigeria.
PG  - 47
LID - 10.11604/pamj.2022.43.47.33346 [doi]
LID - 47
AB  - Sickle cell disease, a genetically inherited blood disorder is a major cause of 
      mortality and morbidity in Nigeria. This condition has significant pathological 
      consequences that result in hemolytic events, induction of inflammatory process, 
      vaso-occlusive episodes, and the stress response that leads to the induction of 
      heat shock protein (HSP) 70. Therefore, this study aimed at correlating the level 
      of serum heat shock protein 70 to haematological parameters in sickle cell 
      subjects. A total of eighty-eight (88) consented participants were recruited for 
      this study, which included apparently healthy persons with homozygous hemoglobin 
      (HbAA 20), heterozygous hemoglobin (HbAS 30), homozygous hemoglobin (HbSS 30), 
      and homozygous hemoglobin (HbSC 08). Subjects are in crisis and steady state. 
      Venous blood samples (5 mls) were collected from subjects in ethylene diamine 
      tetra acetic acid (EDTA) container and analyzed hemoglobin variants using 
      hemoglobin electrophoresis, HSP 70 by Elisa method, and full blood count using 
      standard methods. We demonstrated a significant increase (P<005) in HSP 70 levels 
      of sickle cell disease HbSS and HbSC in steady state and crises when compared to 
      the controls HbAA and HbAS. A significant (p<0.0001) increase noticed in the 
      crisis state is higher than in the steady state. While the mean value of mean 
      corpuscular hemoglobin concentration (MCHC) (35.1+/-43.4), pack cell volume (PCV) 
      (22.4+/-2.7), hemoglobin (Hb) (8.8+/-0.9), absolute neutrophil count (386.4+/-31) and 
      Absolute neutrophil count (7.0+/-2.1) in steady state subjects was significantly 
      higher (p<0.01), as compared to crisis state (29.5+/-2.5, 21.8+/-3.4, 7.3+/-1.8, 
      269.5+/-42 and 6.5+/-2.5) for the respective parameters, whereas, mean corpuscular 
      volume (30.5+/-3.1), white blood cell (16.8+/-3.4), absolute lymphocyte count 
      (5.0+/-1.3) in sickle cell disease subject in crisis state are significantly higher 
      (p<0.01) than in steady state (29.3+/-2.2, 11.3+/-2.8, 4.3+/-1.1) respectively. The 
      mean value of mean corpuscular volume (87.3+/-8.2) in the crisis state was higher 
      when compared to the steady state (83.5+/-7.2) and the mean value of red bloood 
      cell (2.7+/-0.4) in the steady state was higher when compared to the crisis state 
      (2.3+/-0.7). The differences were not significant (p<0.01). These findings suggest 
      that an association exists between Hsp 70 and haematological parameters in sickle 
      cell subjects. This implies that Hsp 70 might be a marker in oxidative stress, 
      hypoxia, vaso-occlusion crisis, and increased serum Hsp 70 levels seem to reflect 
      systemic inflammation. However, further studies are required to determine whether 
      circulating Hsp 70 plays a causative role in the pathogenesis of sickle cell.
CI  - Copyright: Ayodeji Olusola Dickson Olayanju et al.
FAU - Olayanju, Ayodeji Olusola Dickson
AU  - Olayanju AOD
AD  - Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, 
      Nigeria.
FAU - Adeleke, Adedoyin
AU  - Adeleke A
AD  - Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, 
      Nigeria.
FAU - Okolo, Chisara Sylvestina
AU  - Okolo CS
AD  - Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, 
      Nigeria.
FAU - Ogunyemi, Ogunyemi Omotomilola
AU  - Ogunyemi OO
AD  - Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, 
      Nigeria.
FAU - Mary, Ogunjobi Kemisola
AU  - Mary OK
AD  - Department of Medical Laboratory Science, Afe Babalola University, Ado Ekiti, 
      Nigeria.
LA  - eng
PT  - Case Reports
DEP - 20220929
PL  - Uganda
TA  - Pan Afr Med J
JT  - The Pan African medical journal
JID - 101517926
RN  - 0 (HSP70 Heat-Shock Proteins)
RN  - 0 (Hemoglobin, Sickle)
RN  - 0 (Hemoglobins)
SB  - IM
MH  - Humans
MH  - *HSP70 Heat-Shock Proteins
MH  - Nigeria
MH  - *Anemia, Sickle Cell
MH  - Hemoglobin, Sickle
MH  - Hemoglobins/metabolism
PMC - PMC9733451
OTO - NOTNLM
OT  - Sickle cell disease
OT  - haematological parameter
OT  - heat shock protein 70
EDAT- 2022/12/17 06:00
MHDA- 2022/12/20 06:00
PMCR- 2022/09/29
CRDT- 2022/12/16 02:13
PHST- 2022/01/18 00:00 [received]
PHST- 2022/08/31 00:00 [accepted]
PHST- 2022/12/16 02:13 [entrez]
PHST- 2022/12/17 06:00 [pubmed]
PHST- 2022/12/20 06:00 [medline]
PHST- 2022/09/29 00:00 [pmc-release]
AID - PAMJ-43-47 [pii]
AID - 10.11604/pamj.2022.43.47.33346 [doi]
PST - epublish
SO  - Pan Afr Med J. 2022 Sep 29;43:47. doi: 10.11604/pamj.2022.43.47.33346. 
      eCollection 2022.