PMID- 36523959
OWN - NLM
STAT- MEDLINE
DCOM- 20221223
LR  - 20221223
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2022
DP  - 2022
TI  - Amentoflavone Exerts Anti-Neuroinflammatory Effects by Inhibiting 
      TLR4/MyD88/NF-kappaB and Activating Nrf2/HO-1 Pathway in Lipopolysaccharide-Induced 
      BV2 Microglia.
PG  - 5184721
LID - 10.1155/2022/5184721 [doi]
LID - 5184721
AB  - BACKGROUND: Amentoflavone, a natural biflavone, exerts anti-inflammation, 
      antioxidation, and antiapoptosis effects on many diseases. However, the mechanism 
      of amentoflavone on neuroinflammation-related diseases has not been 
      comprehensively examined clearly. METHODS: BV2 microglial cells were treated with 
      amentoflavone (10 muM), followed by lipopolysaccharide (LPS). Microglial 
      activation and migration ability and the expression of proinflammatory cytokines 
      and other signaling proteins were determined using immunohistochemistry, 
      immunofluorescence, quantitative real-time polymerase chain reaction, Western 
      blotting, enzyme-linked immunosorbent assay, and wound-healing assays. RESULTS: 
      Amentoflavone restored LPS-induced microglia activation, migration, and 
      inflammation response which depends on regulating toll-like receptor 4 
      (TLR4)/myeloid differentiation factor 88 (MyD88)/nuclear factor kappa B (NF-kappaB) 
      pathway. In addition, amentoflavone also enhanced nuclear factor erythroid 
      2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) levels in LPS-treated BV2 
      microglial cells. CONCLUSIONS: Amentoflavone ameliorated LPS-induced 
      neuroinflammatory response and oxidative stress in BV2 microglia. These data 
      provide new insight into the mechanism of amentoflavone in the treatment of 
      neuroinflammation-related diseases. Therefore, amentoflavone may be a potential 
      therapeutic option for neurological disorders.
CI  - Copyright (c) 2022 Shikuo Rong et al.
FAU - Rong, Shikuo
AU  - Rong S
AUID- ORCID: 0000-0001-7904-0096
AD  - Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
AD  - Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, 
      Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
AD  - Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key 
      Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
FAU - Yang, Chunrong
AU  - Yang C
AUID- ORCID: 0000-0003-0533-8302
AD  - Department of Gastroenterology, Hospital of Chengdu University of Traditional 
      Chinese Medicine, Chengdu, Sichuan 610072, China.
FAU - Wang, Feng
AU  - Wang F
AUID- ORCID: 0000-0001-7841-5028
AD  - Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key 
      Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
AD  - Department of Neurosurgery, The First Affiliated Hospital of Zhejiang University, 
      Hangzhou, Zhejiang 310003, China.
FAU - Wu, Yiyang
AU  - Wu Y
AUID- ORCID: 0000-0003-1446-0174
AD  - Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key 
      Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
FAU - Sun, Kuishen
AU  - Sun K
AUID- ORCID: 0000-0003-2755-572X
AD  - Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key 
      Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
FAU - Sun, Tao
AU  - Sun T
AUID- ORCID: 0000-0002-8847-6972
AD  - Ningxia Key Laboratory of Cerebrocranial Disease, Incubation Base of National Key 
      Laboratory, Ningxia Medical University, Yinchuan, Ningxia 750004, China.
FAU - Wu, Zeyu
AU  - Wu Z
AUID- ORCID: 0000-0003-0066-0378
AD  - Department of General Surgery, Guangdong Provincial People's Hospital, Guangdong 
      Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
AD  - Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital, 
      Guangdong Academy of Medical Sciences, Guangzhou, Guangdong 510080, China.
LA  - eng
PT  - Journal Article
DEP - 20221206
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 9I1VC79L77 (amentoflavone)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NF-kappa B)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Biflavonoids)
SB  - IM
MH  - Humans
MH  - Cell Line
MH  - Heme Oxygenase-1/drug effects/metabolism
MH  - Lipopolysaccharides/pharmacology/metabolism
MH  - *Microglia/drug effects/metabolism
MH  - Myeloid Differentiation Factor 88/antagonists & inhibitors/metabolism
MH  - Neuroinflammatory Diseases/drug therapy
MH  - NF-E2-Related Factor 2/agonists/metabolism
MH  - NF-kappa B/antagonists & inhibitors/metabolism
MH  - Toll-Like Receptor 4/antagonists & inhibitors/metabolism
MH  - *Biflavonoids/pharmacology/therapeutic use
PMC - PMC9747320
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2022/12/17 06:00
MHDA- 2022/12/20 06:00
PMCR- 2022/12/06
CRDT- 2022/12/16 02:30
PHST- 2022/01/21 00:00 [received]
PHST- 2022/09/27 00:00 [revised]
PHST- 2022/10/17 00:00 [accepted]
PHST- 2022/12/16 02:30 [entrez]
PHST- 2022/12/17 06:00 [pubmed]
PHST- 2022/12/20 06:00 [medline]
PHST- 2022/12/06 00:00 [pmc-release]
AID - 10.1155/2022/5184721 [doi]
PST - epublish
SO  - Mediators Inflamm. 2022 Dec 6;2022:5184721. doi: 10.1155/2022/5184721. 
      eCollection 2022.