PMID- 36526849
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20230902
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Print)
IS  - 0301-4851 (Linking)
VI  - 50
IP  - 2
DP  - 2023 Feb
TI  - Extracellular adenosine 5'-diphosphate promotes MCP-1/CCL2 expression via the 
      P2Y(13) purinergic receptor/ERK signaling axis in temporomandibular joint-derived 
      mouse fibroblast-like synoviocytes.
PG  - 1595-1602
LID - 10.1007/s11033-022-08125-2 [doi]
AB  - BACKGROUND: Temporomandibular joint osteoarthritis (TMJ-OA) causes cartilage 
      degeneration, bone cavitation, and fibrosis of the TMJ. However, the mechanisms 
      underlying the fibroblast-like synoviocyte (FLS)-mediated inflammatory activity 
      in TMJ-OA remain unclear. METHODS AND RESULTS: Reverse transcription-quantitative 
      polymerase chain reaction analysis revealed that the P2Y(1), P2Y(12), and P2Y(13) 
      purinergic receptor agonist adenosine 5'-diphosphate (ADP) significantly induces 
      monocyte chemotactic protein 1 (MCP-1)/ C-C motif chemokine ligand 2 (CCL2) 
      expression in the FLS1 synovial cell line. In contrast, the uracil nucleotide 
      UTP, which is a P2Y(2) and P2Y(4) agonist, has no significant effect on 
      MCP-1/CCL2 production in FLS1 cells. In addition, the P2Y(13) antagonist MRS 2211 
      considerably decreases the expression of ADP-induced MCP-1/CCL2, whereas ADP 
      stimulation enhances extracellular signal-regulated kinase (ERK) phosphorylation. 
      Moreover, it was found that the mitogen-activated protein kinase/ERK kinase (MEK) 
      inhibitor U0126 reduces ADP-induced MCP-1/CCL2 expression. CONCLUSION: ADP 
      enhances MCP-1/CCL2 expression in TMJ FLSs via P2Y(13) receptors in an 
      MEK/ERK-dependent manner, thus resulting in inflammatory cell infiltration in the 
      TMJ. Collectively, the findings of this study contribute to a partial 
      clarification of the signaling pathway underlying the development of inflammation 
      in TMJ-OA and can help identify potential therapeutic targets for suppressing 
      ADP-mediated purinergic signaling in this disease.
CI  - (c) 2022. The Author(s).
FAU - Yokota, Seiji
AU  - Yokota S
AUID- ORCID: 0000-0002-8576-3749
AD  - Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate 
      Medical University, 1-1-1 Idai-dori, Yahaba-cho, Shiwa-gun, 028-3694, Iwate, 
      Japan. syokota@iwate-med.ac.jp.
FAU - Chosa, Naoyuki
AU  - Chosa N
AD  - Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate 
      Medical University, 1-1-1 Idai-dori, Yahaba-cho, Shiwa-gun, 028-3694, Iwate, 
      Japan.
FAU - Matsumoto, Shikino
AU  - Matsumoto S
AD  - Division of Orthodontics, Department of Developmental Oral Health Science, Iwate 
      Medical University, 19-1 Uchimal, 020-8505, Morioka-shi, Iwate, Japan.
FAU - Satoh, Kazuro
AU  - Satoh K
AD  - Division of Orthodontics, Department of Developmental Oral Health Science, Iwate 
      Medical University, 19-1 Uchimal, 020-8505, Morioka-shi, Iwate, Japan.
FAU - Ishisaki, Akira
AU  - Ishisaki A
AD  - Division of Cellular Biosignal Sciences, Department of Biochemistry, Iwate 
      Medical University, 1-1-1 Idai-dori, Yahaba-cho, Shiwa-gun, 028-3694, Iwate, 
      Japan. aishisa@iwate-med.ac.jp.
LA  - eng
GR  - 19K19277/Japan Society for Promotion of Science/
PT  - Journal Article
DEP - 20221216
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Chemokine CCL2)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - 0 (Diphosphates)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Purinergic P2)
RN  - EC 2.7.12.2 (Mitogen-Activated Protein Kinase Kinases)
RN  - K72T3FS567 (Adenosine)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Chemokine CCL2/genetics/metabolism
MH  - Extracellular Signal-Regulated MAP Kinases
MH  - Diphosphates
MH  - *Synoviocytes/metabolism
MH  - Ligands
MH  - *Receptors, Purinergic P2/metabolism
MH  - Mitogen-Activated Protein Kinase Kinases
MH  - Temporomandibular Joint
MH  - Fibroblasts/metabolism
MH  - Adenosine
MH  - Adenosine Diphosphate/pharmacology/metabolism
MH  - Cells, Cultured
PMC - PMC9889505
OTO - NOTNLM
OT  - Extracellular adenosine 5'-diphosphate
OT  - Extracellular signal-regulated kinase
OT  - Fibroblast-like synoviocytes
OT  - Monocyte chemotactic protein 1
OT  - P2Y purinergic receptors
OT  - Temporomandibular joint
COIS- The authors declare that they have no competing interests.
EDAT- 2022/12/17 06:00
MHDA- 2023/02/03 06:00
PMCR- 2022/12/16
CRDT- 2022/12/16 23:38
PHST- 2022/06/13 00:00 [received]
PHST- 2022/11/15 00:00 [accepted]
PHST- 2022/12/17 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/12/16 23:38 [entrez]
PHST- 2022/12/16 00:00 [pmc-release]
AID - 10.1007/s11033-022-08125-2 [pii]
AID - 8125 [pii]
AID - 10.1007/s11033-022-08125-2 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2023 Feb;50(2):1595-1602. doi: 10.1007/s11033-022-08125-2. Epub 
      2022 Dec 16.