PMID- 36527001
OWN - NLM
STAT- MEDLINE
DCOM- 20221220
LR  - 20221222
IS  - 1741-7007 (Electronic)
IS  - 1741-7007 (Linking)
VI  - 20
IP  - 1
DP  - 2022 Dec 17
TI  - A simple high-throughput method for automated detection of Drosophila 
      melanogaster light-dependent behaviours.
PG  - 283
LID - 10.1186/s12915-022-01476-z [doi]
LID - 283
AB  - BACKGROUND: Like most living organisms, the fruit fly Drosophila melanogaster 
      exhibits strong and diverse behavioural reactions to light. Drosophila is a 
      diurnal animal that displays both short- and long-term responses to light, 
      important for, instance, in avoidance and light wavelength preference, regulation 
      of eclosion, courtship, and activity, and provides an important model organism 
      for understanding the regulation of circadian rhythms both at molecular and 
      circuit levels. However, the assessment and comparison of light-based behaviours 
      is still a challenge, mainly due to the lack of a standardised platform to 
      measure behaviour and different protocols created across studies. Here, we 
      describe the Drosophila Interactive System for Controlled Optical manipulations 
      (DISCO), a low-cost, automated, high-throughput device that records the flies' 
      activity using infrared beams while performing LED light manipulations. RESULTS: 
      To demonstrate the effectiveness of this tool and validate its potential as a 
      standard platform, we developed a number of distinct assays, including measuring 
      the locomotor response of flies exposed to sudden darkness (lights-off) stimuli. 
      Both white-eyed and red-eyed wild-type flies exhibit increased activity after the 
      application of stimuli, while no changes can be observed in Fmr1 null allele 
      flies, a model of fragile X syndrome. Next, to demonstrate the use of DISCO in 
      long-term protocols, we monitored the circadian rhythm of the flies for 48 h 
      while performing an alcohol preference test. We show that increased alcohol 
      consumption happens intermittently throughout the day, especially in the dark 
      phases. Finally, we developed a feedback-loop algorithm to implement a place 
      preference test based on the flies' innate aversion to blue light and preference 
      for green light. We show that both white-eyed and red-eyed wild-type flies were 
      able to learn to avoid the blue-illuminated zones. CONCLUSIONS: Our results 
      demonstrate the versatility of DISCO for a range of protocols, indicating that 
      this platform can be used in a variety of ways to study light-dependent 
      behaviours in flies.
CI  - (c) 2022. The Author(s).
FAU - Moulin, Thiago C
AU  - Moulin TC
AUID- ORCID: 0000-0001-7811-5383
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden. thiago.moulin@neuro.uu.se.
AD  - Department of Experimental Medical Science, Lund University, Lund, Sweden. 
      thiago.moulin@neuro.uu.se.
FAU - Dey, Sovik
AU  - Dey S
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Dashi, Giovanna
AU  - Dashi G
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Li, Lei
AU  - Li L
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Sridhar, Vaasudevan
AU  - Sridhar V
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Safa, Tania
AU  - Safa T
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Berkins, Samuel
AU  - Berkins S
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Williams, Michael J
AU  - Williams MJ
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
FAU - Schioth, Helgi B
AU  - Schioth HB
AD  - Department of Surgical Sciences, Functional Pharmacology and Neuroscience, 
      Uppsala University, Uppsala, Sweden.
LA  - eng
GR  - 857394/Horizon 2020/
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221217
PL  - England
TA  - BMC Biol
JT  - BMC biology
JID - 101190720
RN  - 0 (Drosophila Proteins)
RN  - 0 (FMR1 protein, Drosophila)
RN  - 139135-51-6 (Fragile X Mental Retardation Protein)
SB  - IM
MH  - Animals
MH  - *Drosophila melanogaster/physiology
MH  - Circadian Rhythm/physiology
MH  - Drosophila/physiology
MH  - Motor Activity/physiology
MH  - Vision, Ocular
MH  - *Drosophila Proteins
MH  - Fragile X Mental Retardation Protein
PMC - PMC9758938
OTO - NOTNLM
OT  - Activity monitor
OT  - Circadian rhythm
OT  - Ethanol intake
OT  - Jump response
OT  - Light response
OT  - ON/OFF assay
OT  - Operant conditioning
OT  - Spatial learning
OT  - Visual learning
COIS- The authors declare that they have no competing interests.
EDAT- 2022/12/17 06:00
MHDA- 2022/12/21 06:00
PMCR- 2022/12/17
CRDT- 2022/12/16 23:48
PHST- 2021/09/02 00:00 [received]
PHST- 2022/11/23 00:00 [accepted]
PHST- 2022/12/16 23:48 [entrez]
PHST- 2022/12/17 06:00 [pubmed]
PHST- 2022/12/21 06:00 [medline]
PHST- 2022/12/17 00:00 [pmc-release]
AID - 10.1186/s12915-022-01476-z [pii]
AID - 1476 [pii]
AID - 10.1186/s12915-022-01476-z [doi]
PST - epublish
SO  - BMC Biol. 2022 Dec 17;20(1):283. doi: 10.1186/s12915-022-01476-z.