PMID- 36530164
OWN - NLM
STAT- MEDLINE
DCOM- 20230504
LR  - 20240508
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 192
IP  - 1
DP  - 2023 May 2
TI  - An unexpected role for tomato threonine deaminase 2 in host defense against 
      bacterial infection.
PG  - 527-545
LID - 10.1093/plphys/kiac584 [doi]
AB  - The hormones salicylic acid (SA) and jasmonic acid (JA) often act 
      antagonistically in controlling plant defense pathways in response to 
      hemibiotrophs/biotrophs (hemi/biotroph) and herbivores/necrotrophs, respectively. 
      Threonine deaminase (TD) converts threonine to alpha-ketobutyrate and ammonia as the 
      committed step in isoleucine (Ile) biosynthesis and contributes to JA responses 
      by producing the Ile needed to make the bioactive JA-Ile conjugate. Tomato 
      (Solanum lycopersicum) plants have two TD genes: TD1 and TD2. A defensive role 
      for TD2 against herbivores has been characterized in relation to JA-Ile 
      production. However, it remains unknown whether TD2 is also involved in host 
      defense against bacterial hemi/biotrophic and necrotrophic pathogens. Here, we 
      show that in response to the bacterial pathogen-associated molecular pattern 
      (PAMP) flagellin flg22 peptide, an activator of SA-based defense responses, TD2 
      activity is compromised, possibly through carboxy-terminal cleavage. TD2 
      knockdown (KD) plants showed increased resistance to the hemibiotrophic bacterial 
      pathogen Pseudomonas syringae but were more susceptible to the necrotrophic 
      fungal pathogen Botrytis cinerea, suggesting TD2 plays opposite roles in response 
      to hemibiotrophic and necrotrophic pathogens. This TD2 KD plant differential 
      response to different pathogens is consistent with SA- and JA-regulated defense 
      gene expression. flg22-treated TD2 KD plants showed high expression levels of 
      SA-responsive genes, whereas TD2 KD plants treated with the fungal PAMP chitin 
      showed low expression levels of JA-responsive genes. This study indicates TD2 
      acts negatively in defense against hemibiotrophs and positively against 
      necrotrophs and provides insight into a new TD2 function in the elaborate 
      crosstalk between SA and JA signaling induced by pathogen infection.
CI  - (c) American Society of Plant Biologists 2022. All rights reserved. For 
      permissions, please e-mail: journals.permissions@oup.com.
FAU - Yeo, In-Cheol
AU  - Yeo IC
AUID- ORCID: 0000-0001-8562-7213
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
FAU - de Azevedo Manhaes, Ana Marcia Escocard
AU  - de Azevedo Manhaes AME
AUID- ORCID: 0000-0003-0203-6173
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
FAU - Liu, Jun
AU  - Liu J
AUID- ORCID: 0000-0002-9272-9602
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
FAU - Avila, Julian
AU  - Avila J
AUID- ORCID: 0000-0001-9388-3946
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
FAU - He, Ping
AU  - He P
AUID- ORCID: 0000-0002-5926-8349
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
FAU - Devarenne, Timothy P
AU  - Devarenne TP
AUID- ORCID: 0000-0001-6032-8049
AD  - Department of Biochemistry and Biophysics, Texas A&M University, College Station, 
      Texas 77843, USA.
LA  - eng
GR  - R01 GM092893/GM/NIGMS NIH HHS/United States
GR  - R35 GM149197/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (jasmonoyl-isoleucine)
RN  - EC 4.3.1.19 (Threonine Dehydratase)
RN  - 0 (Cyclopentanes)
RN  - 6RI5N05OWW (jasmonic acid)
RN  - 0 (Oxylipins)
RN  - O414PZ4LPZ (Salicylic Acid)
SB  - IM
CIN - Plant Physiol. 2023 May 2;192(1):19-20. PMID: 36722330
MH  - *Solanum lycopersicum/genetics
MH  - Threonine Dehydratase/genetics/metabolism
MH  - Cyclopentanes/pharmacology/metabolism
MH  - Oxylipins/pharmacology/metabolism
MH  - *Bacterial Infections
MH  - Salicylic Acid/pharmacology/metabolism
MH  - Plant Diseases/microbiology
MH  - Gene Expression Regulation, Plant
MH  - Botrytis/physiology
PMC - PMC10152684
COIS- Conflict of interest statement. None declared.
EDAT- 2022/12/20 06:00
MHDA- 2023/05/04 12:41
PMCR- 2023/12/19
CRDT- 2022/12/19 03:23
PHST- 2022/06/28 00:00 [received]
PHST- 2022/11/29 00:00 [accepted]
PHST- 2023/05/04 12:41 [medline]
PHST- 2022/12/20 06:00 [pubmed]
PHST- 2022/12/19 03:23 [entrez]
PHST- 2023/12/19 00:00 [pmc-release]
AID - 6931846 [pii]
AID - kiac584 [pii]
AID - 10.1093/plphys/kiac584 [doi]
PST - ppublish
SO  - Plant Physiol. 2023 May 2;192(1):527-545. doi: 10.1093/plphys/kiac584.