PMID- 36539842 OWN - NLM STAT- MEDLINE DCOM- 20221222 LR - 20221223 IS - 1477-7827 (Electronic) IS - 1477-7827 (Linking) VI - 20 IP - 1 DP - 2022 Dec 20 TI - Insufficient HtrA2 causes meiotic defects in aging germinal vesicle oocytes. PG - 173 LID - 10.1186/s12958-022-01048-4 [doi] LID - 173 AB - BACKGROUND: High-temperature requirement protease A2 (HtrA2/Omi) is a mitochondrial chaperone that is highly conserved from bacteria to humans. It plays an important role in mitochondrial homeostasis and apoptosis. In this study, we investigated the role of HtrA2 in mouse oocyte maturation. METHODS: The role of HtrA2 in mouse oocyte maturation was investigated by employing knockdown (KD) or overexpression (OE) of HtrA2 in young or old germinal vesicle (GV) oocytes. We employed immunoblotting, immunostaining, fluorescent intensity quantification to test the HtrA2 knockdown on the GV oocyte maturation progression, spindle assembly checkpoint, mitochondrial distribution, spindle organization, chromosome alignment, actin polymerization, DNA damage and chromosome numbers and acetylated tubulin levels. RESULTS: We observed a significant reduction in HtrA2 protein levels in aging germinal vesicle (GV) oocytes. Young oocytes with low levels of HtrA2 due to siRNA knockdown were unable to complete meiosis and were partially blocked at metaphase I (MI). They also displayed significantly more BubR1 on kinetochores, indicating that the spindle assembly checkpoint was triggered at MI. Extrusion of the first polar body (Pb1) was significantly less frequent and oocytes with large polar bodies were observed when HtrA2 was depleted. In addition, HtrA2 knockdown induced meiotic spindle/chromosome disorganization, leading to aneuploidy at metaphase II (MII), possibly due to the elevated level of acetylated tubulin. Importantly, overexpression of HtrA2 partially rescued spindle/chromosome disorganization and reduced the rate of aneuploidy in aging GV oocytes. CONCLUSIONS: Collectively, our data suggest that HtrA2 is a key regulator of oocyte maturation, and its deficiency with age appears to contribute to reproduction failure in females. CI - (c) 2022. The Author(s). FAU - Gao, Min AU - Gao M AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. AD - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 51000, China. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. FAU - Qiu, Yanling AU - Qiu Y AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. FAU - Cao, Tianqi AU - Cao T AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. FAU - Li, Dungao AU - Li D AD - The Reproduction Medicine Center of Hui Zhou Municipal Central Hospital, Huizhou, 516001, China. FAU - Wang, Jingwen AU - Wang J AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. FAU - Jiao, Yiren AU - Jiao Y AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. FAU - Chen, Zhiyun AU - Chen Z AD - The Reproduction Medicine Center of Hui Zhou Municipal Central Hospital, Huizhou, 516001, China. 13502435668@139.com. FAU - Huang, Junjiu AU - Huang J AD - MOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. hjunjiu@mail.sysu.edu.cn. AD - Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou, 51000, China. hjunjiu@mail.sysu.edu.cn. AD - Key Laboratory of Reproductive Medicine of Guangdong Province, School of Life Sciences and the First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510275, China. hjunjiu@mail.sysu.edu.cn. LA - eng PT - Journal Article DEP - 20221220 PL - England TA - Reprod Biol Endocrinol JT - Reproductive biology and endocrinology : RB&E JID - 101153627 RN - 0 (Tubulin) SB - IM MH - Female MH - Mice MH - Humans MH - Animals MH - *Tubulin/genetics/metabolism MH - *Oocytes/metabolism MH - Meiosis/genetics MH - Metaphase MH - Aging/genetics PMC - PMC9764539 OTO - NOTNLM OT - Aging OT - Germinal vesicle oocyte OT - HtrA2 OT - Maturation OT - Meiosis COIS- The authors declare that there is no conflict of interest. EDAT- 2022/12/21 06:00 MHDA- 2022/12/23 06:00 PMCR- 2022/12/20 CRDT- 2022/12/20 23:58 PHST- 2022/10/16 00:00 [received] PHST- 2022/12/09 00:00 [accepted] PHST- 2022/12/20 23:58 [entrez] PHST- 2022/12/21 06:00 [pubmed] PHST- 2022/12/23 06:00 [medline] PHST- 2022/12/20 00:00 [pmc-release] AID - 10.1186/s12958-022-01048-4 [pii] AID - 1048 [pii] AID - 10.1186/s12958-022-01048-4 [doi] PST - epublish SO - Reprod Biol Endocrinol. 2022 Dec 20;20(1):173. doi: 10.1186/s12958-022-01048-4.