PMID- 36539950
OWN - NLM
STAT- MEDLINE
DCOM- 20230504
LR  - 20231213
IS  - 1346-8138 (Electronic)
IS  - 0385-2407 (Linking)
VI  - 50
IP  - 5
DP  - 2023 May
TI  - Application of fluorescence in situ hybridization in distinguishing acral 
      melanoma in situ from acral junctional melanocytic nevus on the volar skin in 
      Japanese patients.
PG  - 637-645
LID - 10.1111/1346-8138.16681 [doi]
AB  - Four-colored fluorescence in situ hybridization (FISH) is an ancillary diagnostic 
      tool for melanoma. However, most studies that have investigated the usefulness of 
      FISH primarily focused on advanced melanomas. The aim of the current study was to 
      evaluate the effectiveness of FISH in distinguishing acral melanoma (AM) in situ 
      from benign acral junctional nevus (AJN), two types of lesions that are difficult 
      to differentiate via traditional clinical means. The authors investigated the 
      usefulness of FISH in 91 acral melanocytic lesions, including 50 lesions with 
      diagnostic discrepancies between dermoscopic and pathologic approaches or 
      difficulty diagnosing between AM in situ and AJN, on the volar skin of Japanese 
      patients. The authors classified the lesions based on the diagnosis of 
      dermatologists and pathologists into four groups: (I) lesions with a unanimous 
      diagnosis by dermatologists and pathologists as AM in situ or AJN (n = 41); (II) 
      lesions with a unanimous diagnosis by dermatologists only as AM in situ or AJN 
      (n = 21); (III) lesions with a unanimous diagnosis by pathologists only as AM in 
      situ or AJN (n = 15); and (IV) all other lesions (n = 14). The dermatologists 
      diagnosed the lesions by clinical and dermoscopic photographs alone, while the 
      pathologists diagnosed the lesions by microscopy of hematoxylin and eosin-stained 
      slides alone. In group I (AM in situ [n = 20] and AJN [n = 21]), four-colored 
      FISH demonstrated 90% sensitivity and 81% specificity in distinguishing AM in 
      situ from AJN. There was a significant correlation between the FISH results and 
      the unanimous diagnoses by pathologists alone (p = 0.03) in group III. However, 
      FISH results were not significantly correlated with the unanimous diagnoses by 
      dermatologists alone (p = 0.33) in group II. In conclusion, the four-colored FISH 
      probe kit was useful in distinguishing between AM in situ and AJN and may be an 
      ancillary method when pathologists who are not experts of dermatopathology 
      diagnose melanocytic lesions.
CI  - (c) 2022 Japanese Dermatological Association.
FAU - Takai, Sayaka
AU  - Takai S
AUID- ORCID: 0000-0002-0314-7994
AD  - Department of Dermatology, Saitama Medical University, Saitama, Japan.
AD  - Department of Pathology, Saitama Medical University International Medical Center, 
      Saitama, Japan.
FAU - Arai, Eiichi
AU  - Arai E
AD  - Department of Pathology, Saitama Medical University International Medical Center, 
      Saitama, Japan.
FAU - Shojiguchi, Naoko
AU  - Shojiguchi N
AUID- ORCID: 0000-0001-7732-1433
AD  - Department of Pathology, Saitama Medical University International Medical Center, 
      Saitama, Japan.
FAU - Nakamura, Yasuhiro
AU  - Nakamura Y
AUID- ORCID: 0000-0002-3664-5818
AD  - Department of Skin Oncology/Dermatology, Saitama Medical University International 
      Medical Center, Saitama, Japan.
FAU - Momose, Shuji
AU  - Momose S
AD  - Department of Pathology, Saitama Medical University Saitama Medical Center, 
      Saitama, Japan.
FAU - Fukuda, Tomoo
AU  - Fukuda T
AD  - Department of Dermatology, Saitama Medical University Saitama Medical Center, 
      Saitama, Japan.
FAU - Ishizawa, Keisuke
AU  - Ishizawa K
AD  - Department of Pathology, Saitama Medical University, Saitama, Japan.
FAU - Ogata, Dai
AU  - Ogata D
AD  - Department of Dermatology, Saitama Medical University, Saitama, Japan.
AD  - Department of Dermatologic Oncology, National Cancer Center Hospital, Tokyo, 
      Japan.
FAU - Tsunemi, Yuichiro
AU  - Tsunemi Y
AUID- ORCID: 0000-0003-3590-6489
AD  - Department of Dermatology, Saitama Medical University, Saitama, Japan.
FAU - Nakamura, Koichiro
AU  - Nakamura K
AUID- ORCID: 0000-0002-0367-0312
AD  - Department of Dermatology, Saitama Medical University, Saitama, Japan.
FAU - Tsuchida, Tetsuya
AU  - Tsuchida T
AD  - Department of Dermatology, Saitama Medical University, Saitama, Japan.
LA  - eng
PT  - Journal Article
DEP - 20221220
PL  - England
TA  - J Dermatol
JT  - The Journal of dermatology
JID - 7600545
SB  - IM
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - East Asian People
MH  - Dermoscopy/methods
MH  - *Melanoma/diagnosis/pathology
MH  - *Skin Neoplasms/diagnosis/pathology
MH  - *Nevus, Pigmented/diagnosis/pathology
MH  - Melanoma, Cutaneous Malignant
OTO - NOTNLM
OT  - FISH
OT  - Japanese volar skin
OT  - acral junctional nevus
OT  - acral melanoma in situ
OT  - acral volar melanocytic lesions
OT  - fluorescence in situ hybridization
OT  - melanocytic nevus
OT  - virtual slides
OT  - whole-slide digital imaging
EDAT- 2022/12/22 06:00
MHDA- 2023/05/04 12:41
CRDT- 2022/12/21 00:32
PHST- 2022/11/25 00:00 [revised]
PHST- 2022/08/27 00:00 [received]
PHST- 2022/11/29 00:00 [accepted]
PHST- 2023/05/04 12:41 [medline]
PHST- 2022/12/22 06:00 [pubmed]
PHST- 2022/12/21 00:32 [entrez]
AID - 10.1111/1346-8138.16681 [doi]
PST - ppublish
SO  - J Dermatol. 2023 May;50(5):637-645. doi: 10.1111/1346-8138.16681. Epub 2022 Dec 
      20.