PMID- 36542769
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231026
IS  - 1098-2795 (Electronic)
IS  - 1040-452X (Linking)
VI  - 90
IP  - 7
DP  - 2023 Jul
TI  - Role of gonadotropin-releasing hormone-II and its receptor in swine reproduction.
PG  - 469-479
LID - 10.1002/mrd.23662 [doi]
AB  - The pig represents the only livestock mammal capable of producing a functional 
      protein for the second mammalian form of gonadotropin-releasing hormone (GnRH-II) 
      and its receptor (GnRHR-II). To examine the role of GnRH-II and its receptor in 
      pig reproduction, we produced a unique swine line with ubiquitous knockdown of 
      endogenous GnRHR-II levels (GnRHR-II knockdown [KD]), which is largely the focus 
      of this review. In mature GnRHR-II KD males, circulating testosterone 
      concentrations were 82% lower than littermate control boars, despite similar 
      luteinizing hormone (LH) levels. In addition, nine other gonadal steroids were 
      reduced in the serum of GnRHR-II KD boars, whereas adrenal steroids (except 
      11-deoxycortisol) did not differ between lines. Interestingly, testes from 
      GnRHR-II KD males had fewer, hypertrophic Leydig cells and fewer, enlarged 
      seminiferous tubules than control testes. As expected, downstream reproductive 
      traits such as androgen-dependent organ weights and semen characteristics were 
      also significantly reduced in GnRHR-II KD versus control boars. Next, we explored 
      the importance of this novel ligand/receptor complex in female reproduction. 
      Transgenic gilts had fewer, but heavier, corpora lutea with smaller luteal cells 
      than littermate control females. Although the number of antral follicles were 
      similar between lines, the diameter of antral follicles tended to be larger in 
      GnRHR-II KD females. In regard to steroidogenesis, circulating concentrations of 
      progesterone and 17beta-estradiol were lower in transgenic compared to control 
      gilts, even though serum levels of follicle-stimulating hormone and LH were 
      similar. Thus, GnRH-II and GnRHR-II represent a potential avenue to enhance 
      fertility and promote the profitability of pork producers.
CI  - (c) 2022 Wiley Periodicals LLC.
FAU - White, Brett R
AU  - White BR
AD  - Department of Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska, 
      USA.
FAU - Cederberg, Rebecca A
AU  - Cederberg RA
AD  - Department of Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska, 
      USA.
FAU - Elsken, Dorothy H
AU  - Elsken DH
AD  - Department of Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska, 
      USA.
FAU - Ross, Caitlin E
AU  - Ross CE
AD  - Department of Animal Science, University of Nebraska-Lincoln, Lincoln, Nebraska, 
      USA.
FAU - Lents, Clay A
AU  - Lents CA
AD  - Agricultural Research Service, United States Department of Agriculture, U. S. 
      Meat Animal Research Center, Clay Center, Nebraska, USA.
FAU - Desaulniers, Amy T
AU  - Desaulniers AT
AD  - School of Veterinary Medicine and Biomedical Sciences, University of 
      Nebraska-Lincoln, Lincoln, Nebraska, USA.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Review
DEP - 20221221
PL  - United States
TA  - Mol Reprod Dev
JT  - Molecular reproduction and development
JID - 8903333
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-68-0 (Follicle Stimulating Hormone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Animals
MH  - Female
MH  - Swine
MH  - Male
MH  - *Gonadotropin-Releasing Hormone/metabolism
MH  - *Reproduction
MH  - Follicle Stimulating Hormone/metabolism
MH  - Ovarian Follicle/metabolism
MH  - Animals, Genetically Modified
MH  - Estradiol
MH  - Mammals
OTO - NOTNLM
OT  - GnRH-II
OT  - GnRH-II receptor
OT  - ovary
OT  - steroidogenesis
OT  - testis
EDAT- 2022/12/22 06:00
MHDA- 2023/10/23 12:46
CRDT- 2022/12/21 14:42
PHST- 2022/12/02 00:00 [revised]
PHST- 2022/06/24 00:00 [received]
PHST- 2022/12/06 00:00 [accepted]
PHST- 2023/10/23 12:46 [medline]
PHST- 2022/12/22 06:00 [pubmed]
PHST- 2022/12/21 14:42 [entrez]
AID - 10.1002/mrd.23662 [doi]
PST - ppublish
SO  - Mol Reprod Dev. 2023 Jul;90(7):469-479. doi: 10.1002/mrd.23662. Epub 2022 Dec 21.