PMID- 36542787
OWN - NLM
STAT- MEDLINE
DCOM- 20230119
LR  - 20231021
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Print)
IS  - 0003-2700 (Linking)
VI  - 95
IP  - 2
DP  - 2023 Jan 17
TI  - Quantitative Bottom-Up Glycomic Analysis of Polysaccharides in Food Matrices 
      Using Liquid Chromatography-Tandem Mass Spectrometry.
PG  - 1008-1015
LID - 10.1021/acs.analchem.2c03707 [doi]
AB  - Carbohydrates are the most abundant biomolecules in nature, and specifically, 
      polysaccharides are present in almost all plants and fungi. Due to their 
      compositional diversity, polysaccharide analysis remains challenging. Compared to 
      other biomolecules, high-throughput analysis for carbohydrates has yet to be 
      developed. To address this gap in analytical science, we have developed a 
      multiplexed, high-throughput, and quantitative approach for polysaccharide 
      analysis in foods. Specifically, polysaccharides were depolymerized using a 
      nonenzymatic chemical digestion process followed by oligosaccharide 
      fingerprinting using high performance liquid chromatography-quadrupole 
      time-of-flight mass spectrometry (HPLC-QTOF-MS). Both label-free relative 
      quantitation and absolute quantitation were done based on the abundances of 
      oligosaccharides produced. Method validation included evaluating recovery for a 
      range of polysaccharide standards and a breakfast cereal standard reference 
      material. Nine polysaccharides (starch, cellulose, beta-glucan, mannan, galactan, 
      arabinan, xylan, xyloglucan, chitin) were successfully quantitated with 
      sufficient accuracy (5-25% bias) and high reproducibility (2-15% CV). 
      Additionally, the method was used to identify and quantitate polysaccharides from 
      a diverse sample set of food samples. Absolute concentrations of nine 
      polysaccharides from apples and onions were obtained using an external 
      calibration curve, where varietal differences were observed in some of the 
      samples. The methodology developed in this study will provide complementary 
      polysaccharide-level information to deepen our understanding of the interactions 
      of dietary polysaccharides, gut microbial community, and human health.
FAU - Bacalzo, Nikita P Jr
AU  - Bacalzo NP Jr
AUID- ORCID: 0000-0002-6253-6340
AD  - Department of Chemistry, University of California horizontal line Davis, Davis, California 95616, 
      United States.
FAU - Couture, Garret
AU  - Couture G
AUID- ORCID: 0000-0002-8947-4354
AD  - Department of Chemistry, University of California horizontal line Davis, Davis, California 95616, 
      United States.
FAU - Chen, Ye
AU  - Chen Y
AUID- ORCID: 0000-0002-6379-1968
AD  - Department of Chemistry, University of California horizontal line Davis, Davis, California 95616, 
      United States.
FAU - Castillo, Juan J
AU  - Castillo JJ
AUID- ORCID: 0000-0001-9680-5273
AD  - Department of Chemistry, University of California horizontal line Davis, Davis, California 95616, 
      United States.
FAU - Phillips, Katherine M
AU  - Phillips KM
AD  - Virginia Tech, Blacksburg, Virginia 24061, United States.
FAU - Fukagawa, Naomi K
AU  - Fukagawa NK
AD  - Beltsville Human Nutrition Research Center, USDA Agricultural Research Service, 
      Beltsville, Maryland 20705, United States.
FAU - Lebrilla, Carlito B
AU  - Lebrilla CB
AUID- ORCID: 0000-0001-7190-5323
AD  - Department of Chemistry, University of California horizontal line Davis, Davis, California 95616, 
      United States.
AD  - Department of Biochemistry and Molecular Medicine, University of 
      California horizontal line Davis, Davis, California 95616, United States.
LA  - eng
GR  - R01 DK124193/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20221221
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Polysaccharides)
RN  - 0 (Oligosaccharides)
SB  - IM
MH  - Humans
MH  - *Tandem Mass Spectrometry/methods
MH  - Reproducibility of Results
MH  - *Glycomics
MH  - Polysaccharides/chemistry
MH  - Chromatography, Liquid/methods
MH  - Oligosaccharides/chemistry
MH  - Chromatography, High Pressure Liquid/methods
PMC - PMC9850401
COIS- The authors declare no competing financial interest.
EDAT- 2022/12/22 06:00
MHDA- 2023/01/20 06:00
PMCR- 2023/01/19
CRDT- 2022/12/21 15:02
PHST- 2022/12/22 06:00 [pubmed]
PHST- 2023/01/20 06:00 [medline]
PHST- 2022/12/21 15:02 [entrez]
PHST- 2023/01/19 00:00 [pmc-release]
AID - 10.1021/acs.analchem.2c03707 [doi]
PST - ppublish
SO  - Anal Chem. 2023 Jan 17;95(2):1008-1015. doi: 10.1021/acs.analchem.2c03707. Epub 
      2022 Dec 21.