PMID- 36544920
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221223
IS  - 2378-8038 (Print)
IS  - 2378-8038 (Electronic)
IS  - 2378-8038 (Linking)
VI  - 7
IP  - 6
DP  - 2022 Dec
TI  - CircBFAR correlates with poor prognosis and promotes laryngeal squamous cell 
      cancer progression through miR-31-5p/COL5A1 axis.
PG  - 1951-1962
LID - 10.1002/lio2.966 [doi]
AB  - INTRODUCTION: Laryngeal squamous cell cancer (LSCC) is a highly malignant tumor 
      originating from the respiratory system. Circular RNAs have been reported to be 
      associated with the treatment and prognosis of a variety of cancers, including 
      LSCC. METHODS: The expression of circBFAR, miR-31-5p, and collagen type V alpha 1 
      chain (COL5A1) in LSCC tissues and cells was detected by quantitative real-time 
      polymerase chain reaction. Cell counting kit 8 and 5-Ethynyl-2'-deoxyuridine 
      assays were used to detect cell proliferation. Wound healing assay and transwell 
      assay were used to test cell migration and invasion, respectively. The protein 
      expression in LSCC cells was detected with western blot. The relationships 
      between miR-31-5p and circBFAR or COL5A1 were identified by dual-luciferase 
      reporter assay, RNA-pull down assay, and immunoprecipitation assay. The effect of 
      circBFAR on tumor growth in vivo was detected by tumor xenograft mice experiment. 
      The protein expression of COL5A1 and KI-67 in LSCC tissues was measured by 
      immunohistochemistry assay. RESULTS: CircBFAR was increased in LSCC tissues and 
      cells, and was related to advanced clinical stage and overall survival of LSCC 
      patients. The cell viability and proliferation were inhibited by circBFAR 
      knockdown and silencing of circBFAR blocked migration and invasion of LSCC cells. 
      CircBFAR knockdown suppressed cell tube formation, and the protein expression of 
      KI-67, matrix metallopeptidase 2 (MMP2), and vascular endothelial growth factor A 
      (VEGFA) in LSCC cells. MiR-31-5p was the target of circBFAR, and the inhibitory 
      effects of circBFAR deficiency on viability, proliferation, migration, invasion, 
      tube formation and the protein expression of KI-67, MMP2, and VEGFA in LSCC cells 
      were rescued by miR-31-5p downregulation. COL5A1 was negatively regulated by 
      miR-31-5p, and was boosted in LSCC tissues and cells. COL5A1 overexpression 
      reversed the inhibitory effects of miR-31-5p on LSCC cells. CircBFAR 
      insufficiency hindered tumor growth in vivo. CONCLUSION: CircBFAR, miR-31-5p, and 
      COL5A1 in LSCC progression might provide novel therapeutic targets for LSCC 
      clinical intervention.
CI  - (c) 2022 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley 
      Periodicals LLC on behalf of The Triological Society.
FAU - Gong, Hengcui
AU  - Gong H
AD  - Department of Otolaryngology Yibin Hospital of Traditional Chinese Medicine Yibin 
      Sichuan China.
FAU - Wu, Wei
AU  - Wu W
AD  - Department of Radiotherapy GanZhou Cancer Hospital/The Affiliated Cancer Hospital 
      of Gannan Medical University GanZhou Jiangxi China.
FAU - Fang, Chuankai
AU  - Fang C
AD  - Department of Ophthalmology Tongxiang First people's Hospital Tongxiang Zhejiang 
      China.
FAU - He, Di
AU  - He D
AUID- ORCID: 0000-0002-8927-4555
AD  - Department of Otorhinolaryngology Tongxiang First people's Hospital Tongxiang 
      Zhejiang China.
LA  - eng
PT  - Journal Article
DEP - 20221103
PL  - United States
TA  - Laryngoscope Investig Otolaryngol
JT  - Laryngoscope investigative otolaryngology
JID - 101684963
PMC - PMC9764770
OTO - NOTNLM
OT  - COL5A1
OT  - CircBFAR
OT  - laryngeal squamous cell cancer
OT  - miR-31-5p
COIS- The author declares that there is no conflict of interest that could be perceived 
      as prejudicing the impartiality of the research reported.
EDAT- 2022/12/23 06:00
MHDA- 2022/12/23 06:01
PMCR- 2022/11/03
CRDT- 2022/12/22 02:26
PHST- 2022/01/24 00:00 [received]
PHST- 2022/06/27 00:00 [revised]
PHST- 2022/07/21 00:00 [accepted]
PHST- 2022/12/22 02:26 [entrez]
PHST- 2022/12/23 06:00 [pubmed]
PHST- 2022/12/23 06:01 [medline]
PHST- 2022/11/03 00:00 [pmc-release]
AID - LIO2966 [pii]
AID - 10.1002/lio2.966 [doi]
PST - epublish
SO  - Laryngoscope Investig Otolaryngol. 2022 Nov 3;7(6):1951-1962. doi: 
      10.1002/lio2.966. eCollection 2022 Dec.