PMID- 36548206
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 1873-149X (Electronic)
IS  - 0928-4680 (Print)
IS  - 0928-4680 (Linking)
VI  - 29
IP  - 4
DP  - 2022 Nov 23
TI  - The Effect of TGFbeta1 in Adipocyte on Inflammatory and Fibrotic Markers at 
      Different Stages of Adipocyte Differentiation.
PG  - 640-649
LID - 10.3390/pathophysiology29040050 [doi]
AB  - Transforming growth factor beta (TGFbeta) is a versatile cytokine. Although a 
      profibrotic role of TGFbeta is well established, its effect on tissue inhibitor of 
      metalloproteinase (TIMPs) and inflammatory mediators are incompletely described. 
      This study investigates the profibrotic and pro-inflammatory role of TGFbeta1 during 
      adipocyte differentiation. NIH3T3L1 cells were used for the in vitro study and 
      were differentiated by adding a standard differentiation mix either with 
      rosiglitazone (R-Diff) or without (S-Diff). Recombinant TGFbeta1 (2 ng/mL) was added 
      to the undifferentiated preadipocyte during the commitment stage and at the 
      terminal differentiation stage. TGFbeta1 treatment significantly decreased 
      adiponectin mRNA at both early commitment (>300 fold) and terminal differentiated 
      cells [S-Diff (~33%) or R-Diff (~20%)]. TGFbeta1 upregulated collagen VI mRNA and 
      its regulators connective tissue growth factor (CCN2/CTGF), TIMP1 and TIMP3 mRNA 
      levels in undifferentiated preadipocytes and adipocytes at commitment stage. But 
      in the terminal differentiated adipocytes, changes in mRNA and protein of 
      collagen VI and TIMP3 mRNA were not observed despite an increase in CCN2/CTGF, 
      TIMP1 mRNA. Although TGFbeta1 upregulated interleukin-6 (IL6) and monocyte 
      chemoattractant protein-1 (MCP1) mRNA at all stages of differentiation, decreased 
      tumor necrosis factor-alpha (TNFalpha) mRNA was observed early in adipocyte 
      differentiation. This study highlights the complex role of TGFbeta1 on extracellular 
      matrix (ECM) remodeling and inflammatory markers in stimulating both synthetic 
      and inhibitory markers of fibrosis at different stages of adipocyte 
      differentiation.
FAU - Maharjan, Babu Raja
AU  - Maharjan BR
AD  - Greg Brown Diabetes & Endocrinology Laboratory, Sydney Medical School, University 
      of Sydney, Sydney, NSW 2006, Australia.
AD  - School of Medicine, Department of Biochemistry, Patan Academy of Health Sciences, 
      Lalitpur 44700, Nepal.
FAU - McLennan, Susan V
AU  - McLennan SV
AD  - Greg Brown Diabetes & Endocrinology Laboratory, Sydney Medical School, University 
      of Sydney, Sydney, NSW 2006, Australia.
AD  - New South Wales Health Pathology, Sydney, NSW 2050, Australia.
FAU - Twigg, Stephen M
AU  - Twigg SM
AD  - Greg Brown Diabetes & Endocrinology Laboratory, Sydney Medical School, University 
      of Sydney, Sydney, NSW 2006, Australia.
AD  - Department of Endocrinology, Royal Prince Alfred Hospital, Sydney, NSW 2006, 
      Australia.
FAU - Williams, Paul F
AU  - Williams PF
AUID- ORCID: 0000-0002-8654-1697
AD  - Greg Brown Diabetes & Endocrinology Laboratory, Sydney Medical School, University 
      of Sydney, Sydney, NSW 2006, Australia.
LA  - eng
GR  - Australia Award/Government of Australia/
GR  - Kellion Diabetes Fund in the Sydney Medical School Foundation/The University of 
      Sydney/
GR  - Endocrinology Trust Fund/Royal Prince Alfred Hospital Sydney/
PT  - Journal Article
DEP - 20221123
PL  - Switzerland
TA  - Pathophysiology
JT  - Pathophysiology : the official journal of the International Society for 
      Pathophysiology
JID - 9433813
PMC - PMC9788619
OTO - NOTNLM
OT  - TGFbeta
OT  - adipocyte
OT  - fibrosis
OT  - inflammation
OT  - stages of differentiation
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/23 06:00
MHDA- 2022/12/23 06:01
PMCR- 2022/11/23
CRDT- 2022/12/22 13:12
PHST- 2022/10/20 00:00 [received]
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/11/21 00:00 [accepted]
PHST- 2022/12/22 13:12 [entrez]
PHST- 2022/12/23 06:00 [pubmed]
PHST- 2022/12/23 06:01 [medline]
PHST- 2022/11/23 00:00 [pmc-release]
AID - pathophysiology29040050 [pii]
AID - pathophysiology-29-00050 [pii]
AID - 10.3390/pathophysiology29040050 [doi]
PST - epublish
SO  - Pathophysiology. 2022 Nov 23;29(4):640-649. doi: 10.3390/pathophysiology29040050.