PMID- 36548865
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221226
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 9
IP  - 12
DP  - 2022 Dec 18
TI  - Activin A Reduces Porcine Granulosa Cells Apoptosis via ERbeta-Dependent ROS 
      Modulation.
LID - 10.3390/vetsci9120704 [doi]
LID - 704
AB  - Unfavorable conditions compromise animal reproduction by altering the ovarian 
      granulosa cells' follicular dynamics and normal physiological function (GCs), 
      eventually resulting in oxidative damage and cell apoptosis. Activin is produced 
      in the GCs and plays a vital role in folliculogenesis. This study investigated 
      the effects of activin A (ACT-A) treatment in vitro on the apoptosis of porcine 
      GCs and the underlying molecular mechanism. We found that ACT-A could attenuate 
      the apoptosis of the GCs and enhance the synthesis of estrogen (E2). ACT-A also 
      enhanced FSH-induced estrogen receptor-beta (ERbeta) expression, inhibiting ERbeta 
      aggravated intracellular accumulation of the reactive oxygen species (ROS) and 
      apoptosis. The E2 levels in the culture medium, the mRNA expression pattern of 
      the apoptosis-related genes (CASPASE 3, BCL2, and BAX), steroidogenesis-related 
      gene (CYP19A1), and cell viability were analyzed to confirm the results. In 
      summary, this study indicated the protective role of ACT-A in apoptosis by 
      attenuating the ROS accumulation through ERbeta. These results aim to enhance the 
      follicular functions and improve animal reproductive performance.
FAU - Chen, Fang
AU  - Chen F
AD  - Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 
      210014, China.
AD  - Key laboratory of Crop and Animal Integrated Farming, Ministry of Agriculture, 
      Nanjing 210014, China.
FAU - Zhu, Xiaoqing
AU  - Zhu X
AD  - Science and Technology Industry Development Center, Chongqing Medical and 
      Pharmaceutical College, Chongqing 401331, China.
LA  - eng
GR  - BK20190256/Natural Science Foundation of Jiangsu Province/
GR  - cstc2020jcyj-msxmX0369/Natural Science Foundation of Chongqing/
GR  - ygz2021105/The grant of Chongqing Medical and Pharmaceutical College/
PT  - Journal Article
DEP - 20221218
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC9783085
OTO - NOTNLM
OT  - activin A
OT  - apoptosis
OT  - estrogen receptor beta (ERbeta)
OT  - porcine granulosa cells
OT  - reactive oxygen species
COIS- The authors say they have no competing interest. The study's design, publication 
      choice, and manuscript preparation were all made independently of the sponsors.
EDAT- 2022/12/23 06:00
MHDA- 2022/12/23 06:01
PMCR- 2022/12/18
CRDT- 2022/12/22 15:43
PHST- 2022/11/01 00:00 [received]
PHST- 2022/12/13 00:00 [revised]
PHST- 2022/12/14 00:00 [accepted]
PHST- 2022/12/22 15:43 [entrez]
PHST- 2022/12/23 06:00 [pubmed]
PHST- 2022/12/23 06:01 [medline]
PHST- 2022/12/18 00:00 [pmc-release]
AID - vetsci9120704 [pii]
AID - vetsci-09-00704 [pii]
AID - 10.3390/vetsci9120704 [doi]
PST - epublish
SO  - Vet Sci. 2022 Dec 18;9(12):704. doi: 10.3390/vetsci9120704.