PMID- 36550973
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221225
IS  - 2306-5354 (Print)
IS  - 2306-5354 (Electronic)
IS  - 2306-5354 (Linking)
VI  - 9
IP  - 12
DP  - 2022 Dec 4
TI  - Application of Precision-Cut Lung Slices as an In Vitro Model for Research of 
      Inflammatory Respiratory Diseases.
LID - 10.3390/bioengineering9120767 [doi]
LID - 767
AB  - The leading cause of many respiratory diseases is an ongoing and progressive 
      inflammatory response. Traditionally, inflammatory lung diseases were studied 
      primarily through animal models, cell cultures, and organoids. These technologies 
      have certain limitations, despite their great contributions to the study of 
      respiratory diseases. Precision-cut lung slices (PCLS) are thin, uniform tissue 
      slices made from human or animal lung tissue and are widely used extensively both 
      nationally and internationally as an in vitro organotypic model. Human lung 
      slices bridge the gap between in vivo and in vitro models, and they can replicate 
      the living lung environment well while preserving the lungs' basic structures, 
      such as their primitive cells and trachea. However, there is no perfect model 
      that can completely replace the structure of the human lung, and there is still a 
      long way to go in the research of lung slice technology. This review details and 
      analyzes the strengths and weaknesses of precision lung slices as an in vitro 
      model for exploring respiratory diseases associated with inflammation, as well as 
      recent advances in this field.
FAU - Liu, Yan
AU  - Liu Y
AD  - Anesthesiology Department, Dalian Medical University, Dalian 116041, China.
FAU - Wu, Ping
AU  - Wu P
AD  - Department of Anesthesiology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian 116014, China.
FAU - Wang, Yin
AU  - Wang Y
AD  - Anesthesiology Department, Dalian Medical University, Dalian 116041, China.
FAU - Liu, Yansong
AU  - Liu Y
AD  - Anesthesiology Department, Dalian Medical University, Dalian 116041, China.
FAU - Yang, Hongfang
AU  - Yang H
AD  - Department of Anesthesiology, Dalian University Affiliated Xinhua Hospital, 
      Dalian 116021, China.
FAU - Zhou, Guohua
AU  - Zhou G
AD  - Ningbo First Hospital, Ningbo 315016, China.
FAU - Wu, Xiaoqi
AU  - Wu X
AD  - Department of Anesthesiology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian 116014, China.
FAU - Wen, Qingping
AU  - Wen Q
AD  - Department of Anesthesiology, The First Affiliated Hospital of Dalian Medical 
      University, Dalian 116014, China.
LA  - eng
GR  - LJKZ0851 to QP W/Educational Department of Liaoning Province/
PT  - Journal Article
PT  - Review
DEP - 20221204
PL  - Switzerland
TA  - Bioengineering (Basel)
JT  - Bioengineering (Basel, Switzerland)
JID - 101676056
PMC - PMC9774555
OTO - NOTNLM
OT  - ex vivo lung models
OT  - human lung tissue
OT  - lung inflammation
OT  - precision-cut lung slices
OT  - respiratory system
COIS- The authors have no relevant financial or non-financial interests to disclose.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/12/04
CRDT- 2022/12/23 01:07
PHST- 2022/10/06 00:00 [received]
PHST- 2022/11/30 00:00 [revised]
PHST- 2022/12/02 00:00 [accepted]
PHST- 2022/12/23 01:07 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/12/04 00:00 [pmc-release]
AID - bioengineering9120767 [pii]
AID - bioengineering-09-00767 [pii]
AID - 10.3390/bioengineering9120767 [doi]
PST - epublish
SO  - Bioengineering (Basel). 2022 Dec 4;9(12):767. doi: 10.3390/bioengineering9120767.