PMID- 36551893
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221225
IS  - 2227-9059 (Print)
IS  - 2227-9059 (Electronic)
IS  - 2227-9059 (Linking)
VI  - 10
IP  - 12
DP  - 2022 Dec 5
TI  - Polyelectrolyte Coating of Ferumoxytol Differentially Impacts the Labeling of 
      Inflammatory and Steady-State Dendritic Cell Subtypes.
LID - 10.3390/biomedicines10123137 [doi]
LID - 3137
AB  - Engineered magnetic nanoparticles (MNPs) are emerging as advanced tools for 
      medical applications. The coating of MNPs using polyelectrolytes (PEs) is a 
      versatile means to tailor MNP properties and is used to optimize MNP 
      functionality. Dendritic cells (DCs) are critical regulators of adaptive immune 
      responses. Functionally distinct DC subsets exist, either under steady-state or 
      inflammatory conditions, which are explored for the specific treatment of various 
      diseases, such as cancer, autoimmunity, and transplant rejection. Here, the 
      impact of the PE coating of ferumoxytol for uptake into both inflammatory and 
      steady-state DCs and the cellular responses to MNP labeling is addressed. 
      Labeling efficiency by uncoated and PE-coated ferumoxytol is highly variable in 
      different DC subsets, and PE coating significantly improves the labeling of 
      steady-state DCs. Uncoated ferumoxytol results in increased cytotoxicity of 
      steady-state DCs after labeling, which is abolished by the PE coating, while no 
      increased cell death is observed in inflammatory DCs. Furthermore, uncoated and 
      PE-coated ferumoxytol appear immunologically inert in inflammatory DCs, but they 
      induce activation of steady-state DCs. These results show that the PE coating of 
      MNPs can be applied to endow particles with desired properties for enhanced 
      uptake and cell type-specific responses in distinct target DC populations.
FAU - Celikkin, Nehar
AU  - Celikkin N
AUID- ORCID: 0000-0001-8858-2057
AD  - Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen 
      University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
AD  - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 
      Pauwelsstrasse 20, 52074 Aachen, Germany.
FAU - Wong, John E
AU  - Wong JE
AD  - Institute of Physical Chemistry, RWTH Aachen University, Landoltweg 2, 52056 
      Aachen, Germany.
FAU - Zenke, Martin
AU  - Zenke M
AUID- ORCID: 0000-0002-1107-3251
AD  - Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen 
      University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
AD  - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 
      Pauwelsstrasse 20, 52074 Aachen, Germany.
FAU - Hieronymus, Thomas
AU  - Hieronymus T
AUID- ORCID: 0000-0002-7879-6518
AD  - Institute for Biomedical Engineering, Department of Cell Biology, RWTH Aachen 
      University Hospital, Pauwelsstrasse 30, 52074 Aachen, Germany.
AD  - Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, 
      Pauwelsstrasse 20, 52074 Aachen, Germany.
AD  - Institute for Cell and Tumor Biology, RWTH Aachen University Hospital, 
      Pauwelsstrasse 30, 52074 Aachen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20221205
PL  - Switzerland
TA  - Biomedicines
JT  - Biomedicines
JID - 101691304
PMC - PMC9776020
OTO - NOTNLM
OT  - dendritic cells
OT  - ferumoxytol
OT  - nanoparticles
OT  - polyelectrolytes
OT  - tracking
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/12/05
CRDT- 2022/12/23 01:12
PHST- 2022/11/10 00:00 [received]
PHST- 2022/12/02 00:00 [revised]
PHST- 2022/12/03 00:00 [accepted]
PHST- 2022/12/23 01:12 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/12/05 00:00 [pmc-release]
AID - biomedicines10123137 [pii]
AID - biomedicines-10-03137 [pii]
AID - 10.3390/biomedicines10123137 [doi]
PST - epublish
SO  - Biomedicines. 2022 Dec 5;10(12):3137. doi: 10.3390/biomedicines10123137.