PMID- 36552612
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221225
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 12
DP  - 2022 Dec 4
TI  - Broccoli-Derived Glucoraphanin Activates AMPK/PGC1alpha/NRF2 Pathway and Ameliorates 
      Dextran-Sulphate-Sodium-Induced Colitis in Mice.
LID - 10.3390/antiox11122404 [doi]
LID - 2404
AB  - As the prevalence of inflammatory bowel diseases (IBD) rises, the etiology of IBD 
      draws increasing attention. Glucoraphanin (GRP), enriched in cruciferous 
      vegetables, is a precursor of sulforaphane, known to have anti-inflammatory and 
      antioxidative effects. We hypothesized that dietary GRP supplementation can 
      prevent mitochondrial dysfunction and oxidative stress in an acute colitis mouse 
      model induced by dextran sulfate sodium (DSS). Eight-week-old mice were fed a 
      regular rodent diet either supplemented with or without GRP. After 4 weeks of 
      dietary treatments, half of the mice within each dietary group were subjected to 
      2.5% DSS treatment to induce colitis. Dietary GRP decreased DSS-induced body 
      weight loss, disease activity index, and colon shortening. Glucoraphanin 
      supplementation protected the colonic histological structure, suppressed 
      inflammatory cytokines, interleukin (IL)-1beta, IL-18, and tumor necrosis factor-alpha 
      (TNF-alpha), and reduced macrophage infiltration in colonic tissues. Consistently, 
      dietary GRP activated AMP-activated protein kinase (AMPK), peroxisome 
      proliferator-activated receptor-gamma coactivator (PGC)-1alpha, and nuclear factor 
      erythroid 2-related factor 2 (NRF2) pathways in the colonic tissues of 
      DSS-treated mice, which was associated with increased mitochondrial DNA and 
      decreased content of the oxidative product 8-hydroxydeoxyguanosine (8-OHDG), a 
      nucleotide oxidative product of DNA. In conclusion, dietary GRP attenuated 
      mitochondrial dysfunction, inflammatory response, and oxidative stress induced by 
      DSS, suggesting that dietary GRP provides a dietary strategy to alleviate IBD 
      symptoms.
FAU - Tian, Qiyu
AU  - Tian Q
AD  - School of Food Science, Washington State University, Pullman, WA 99164, USA.
AD  - Department of Animal Sciences, Washington State University, Pullman, WA 99164, 
      USA.
FAU - Xu, Zhixin
AU  - Xu Z
AD  - School of Food Science, Washington State University, Pullman, WA 99164, USA.
FAU - Sun, Qi
AU  - Sun Q
AUID- ORCID: 0000-0002-5434-1324
AD  - School of Food Science, Washington State University, Pullman, WA 99164, USA.
FAU - Iniguez, Alejandro Bravo
AU  - Iniguez AB
AD  - School of Food Science, Washington State University, Pullman, WA 99164, USA.
FAU - Du, Min
AU  - Du M
AD  - Department of Animal Sciences, Washington State University, Pullman, WA 99164, 
      USA.
FAU - Zhu, Mei-Jun
AU  - Zhu MJ
AD  - School of Food Science, Washington State University, Pullman, WA 99164, USA.
LA  - eng
GR  - 2018-67017-27517/USDA-National Institute of Food and Agriculture (USDA-NIFA)/
PT  - Journal Article
DEP - 20221204
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9774969
OTO - NOTNLM
OT  - DSS
OT  - NRF2
OT  - glucoraphanin
OT  - inflammatory bowel diseases
OT  - mitochondrial homeostasis
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/12/04
CRDT- 2022/12/23 01:16
PHST- 2022/11/04 00:00 [received]
PHST- 2022/11/30 00:00 [revised]
PHST- 2022/12/01 00:00 [accepted]
PHST- 2022/12/23 01:16 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/12/04 00:00 [pmc-release]
AID - antiox11122404 [pii]
AID - antioxidants-11-02404 [pii]
AID - 10.3390/antiox11122404 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Dec 4;11(12):2404. doi: 10.3390/antiox11122404.