PMID- 36552636
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230308
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 11
IP  - 12
DP  - 2022 Dec 9
TI  - Hatched Eggshell Membrane Can Be a Novel Source of Antioxidant Hydrolysates to 
      Protect against H(2)O(2)-Induced Oxidative Stress in Human Chondrocytes.
LID - 10.3390/antiox11122428 [doi]
LID - 2428
AB  - Natural antioxidants derived from agricultural by-products have great promise and 
      ecological advantages in the treatment of oxidative stress-related diseases. The 
      eggshell membrane (ESM) from hatched eggs, i.e., the hatched ESM, is a globally 
      abundant agricultural byproduct, and its high-value utilization has been rarely 
      studied compared to the well-studied ESM from fresh eggs. In this research, we 
      systematically characterized the hatched ESM as a novel source of antioxidant 
      hydrolysates and explored their potential role in H2O2-induced human 
      chondrocytes. The results showed that the hatched ESM is a protein-rich fibrous 
      mesh material with a significantly different structure and composition from those 
      of fresh ESM. Enzymatic hydrolysis of hatched ESM can produce antioxidant 
      hydrolysates rich in low molecular weight (MW) peptides, which mainly derived 
      from the Lysyl oxidase homolog by Nano-LC-MS/MS analysis. The peptide fraction 
      with MW < 3 kDa (HEMH-I) exhibited the highest DPPH radical scavenging, 
      Fe2+-chelating, and Fe3+-reducing abilities. In H2O2-induced human SW1353 
      chondrocytes, HEMH-I treatment significantly increased the cell viability and 
      ameliorated oxidative stress, inflammatory response, and cartilage matrix 
      degradation by reducing the level of ROS, matrix metalloprotease 3 (MMP3), MMP13, 
      and IL-6, and by promoting the expression of SOD and type II collagen, 
      potentially through activating the cellular Keap1/Nrf2/HO-1 pathway. This study 
      provides a theoretical basis for the value-added application of hatched ESM waste 
      to produce antioxidant hydrolysates and indicates their potential as functional 
      food and pharmaceuticals.
FAU - Zhu, Lingjiao
AU  - Zhu L
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
AD  - National R&D Branch Center for Egg Processing, Jingmen 431800, China.
FAU - Ma, Meihu
AU  - Ma M
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Ahn, Dong Uk
AU  - Ahn DU
AD  - Animal Science Department, Iowa State University, Ames, IO 50011, USA.
FAU - Guyonnet, Vincent
AU  - Guyonnet V
AD  - FFI Consulting, Limited, 2488 Lyn Road, Brockville, ON K6V 5T3, Canada.
FAU - Wang, Limei
AU  - Wang L
AD  - National R&D Branch Center for Egg Processing, Jingmen 431800, China.
FAU - Zheng, Yuting
AU  - Zheng Y
AD  - National R&D Branch Center for Egg Processing, Jingmen 431800, China.
FAU - He, Qin
AU  - He Q
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Xiong, Hanguo
AU  - Xiong H
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Huang, Xi
AU  - Huang X
AUID- ORCID: 0000-0001-7380-8236
AD  - College of Food Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
LA  - eng
GR  - 32072237/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20221209
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC9774709
OTO - NOTNLM
OT  - Keap1/Nrf2/HO-1 pathway
OT  - antioxidant hydrolysates
OT  - chondrocytes
OT  - hatched eggshell membrane
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/12/09
CRDT- 2022/12/23 01:16
PHST- 2022/09/17 00:00 [received]
PHST- 2022/11/30 00:00 [revised]
PHST- 2022/12/03 00:00 [accepted]
PHST- 2022/12/23 01:16 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/12/09 00:00 [pmc-release]
AID - antiox11122428 [pii]
AID - antioxidants-11-02428 [pii]
AID - 10.3390/antiox11122428 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2022 Dec 9;11(12):2428. doi: 10.3390/antiox11122428.