PMID- 36552874
OWN - NLM
STAT- MEDLINE
DCOM- 20221226
LR  - 20230115
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 11
IP  - 24
DP  - 2022 Dec 17
TI  - The Association between Maternal Oral Inflammation and Neutrophil Phenotypes and 
      Poly-Unsaturated Fatty Acids Composition in Human Milk: A Prospective Cohort 
      Study.
LID - 10.3390/cells11244110 [doi]
LID - 4110
AB  - This prospective cohort study aimed to investigate the impact of maternal oral 
      inflammation on human milk composition including neutrophil counts, activation 
      state (based on cluster of differentiation (CD) markers expression), and fatty 
      acid levels. Fifty mothers were recruited from St. Michael's hospital, Toronto, 
      and followed up from 2-4 weeks until 4 months postpartum. Oral rinse and human 
      milk samples were collected at both timepoints. Oral polymorphonuclear 
      neutrophils (oPMNs) within the rinses were quantified using flow cytometry and 
      the participants' oral health state was categorized into three groups (i.e., 
      healthy, moderate, and severe) based on the oPMNs counts. Fatty acids were 
      identified and quantified using a gas chromatography-flame ionization detector 
      (GC-FID). Compared to mothers with a healthy oral health state, mothers with 
      moderate to severe oral inflammation had a statistically significant decrease in 
      the expression of CD64 biomarker, an increase in the expression of CD14 biomarker 
      on human milk neutrophils and a decrease in the levels of eicosapentaenoic acid 
      (C20:5n-3) in their human milk at follow-up compared to baseline. This study 
      demonstrates for the first time that maternal oral inflammation can affect human 
      milk composition. The mechanism by which these alterations can affect infant 
      health outcomes in the long term critically needs to be considered.
FAU - Badewy, Rana
AU  - Badewy R
AD  - Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada.
FAU - Azarpazhooh, Amir
AU  - Azarpazhooh A
AD  - Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada.
AD  - Department of Dentistry, Centre for Advanced Dental Research and Care, Mount 
      Sinai Hospital, Toronto, ON M5G 1X5, Canada.
FAU - Tenenbaum, Howard
AU  - Tenenbaum H
AD  - Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada.
AD  - Department of Dentistry, Centre for Advanced Dental Research and Care, Mount 
      Sinai Hospital, Toronto, ON M5G 1X5, Canada.
FAU - Connor, Kristin L
AU  - Connor KL
AD  - Department of Health Sciences, Carleton University, Ottawa, ON K1S 5B6, Canada.
FAU - Lai, Jim Yuan
AU  - Lai JY
AD  - Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada.
FAU - Sgro, Michael
AU  - Sgro M
AD  - Department of Pediatrics, St. Michael's Hospital, Unity Health Toronto, Toronto, 
      ON M5B 1W8, Canada.
AD  - Department of Pediatrics, University of Toronto, Toronto, ON M5G 1X3, Canada.
FAU - Bazinet, Richard P
AU  - Bazinet RP
AD  - Department of Nutritional Sciences, University of Toronto, Toronto, ON M5S 1A8, 
      Canada.
FAU - Fine, Noah
AU  - Fine N
AD  - Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON 
      M5G 1X3, Canada.
FAU - Watson, Erin
AU  - Watson E
AD  - Department of Dental Oncology, Princess Margaret Cancer Centre, Toronto, ON M5G 
      2C1, Canada.
FAU - Sun, Chunxiang
AU  - Sun C
AD  - Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON 
      M5G 1X3, Canada.
FAU - Saha, Sourav
AU  - Saha S
AD  - Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON 
      M5G 1X3, Canada.
FAU - Glogauer, Michael
AU  - Glogauer M
AUID- ORCID: 0000-0002-6248-5977
AD  - Faculty of Dentistry, University of Toronto, Toronto, ON M5G 1X3, Canada.
AD  - Matrix Dynamics Group, Faculty of Dentistry, University of Toronto, Toronto, ON 
      M5G 1X3, Canada.
AD  - Department of Dental Oncology, Princess Margaret Cancer Centre, Toronto, ON M5G 
      2C1, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221217
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Infant
MH  - Female
MH  - Humans
MH  - *Neutrophils/metabolism
MH  - *Milk, Human/metabolism
MH  - Prospective Studies
MH  - Fatty Acids, Unsaturated/metabolism
MH  - Phenotype
MH  - Biomarkers/metabolism
MH  - Inflammation/metabolism
PMC - PMC9777263
OTO - NOTNLM
OT  - breast milk
OT  - breastfeeding
OT  - gingivitis
OT  - human milk
OT  - neutrophils
OT  - oral inflammatory load
OT  - oral polymorphonuclear neutrophils
OT  - periodontal diseases
OT  - periodontitis
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/27 06:00
PMCR- 2022/12/17
CRDT- 2022/12/23 01:18
PHST- 2022/11/11 00:00 [received]
PHST- 2022/12/09 00:00 [revised]
PHST- 2022/12/15 00:00 [accepted]
PHST- 2022/12/23 01:18 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/27 06:00 [medline]
PHST- 2022/12/17 00:00 [pmc-release]
AID - cells11244110 [pii]
AID - cells-11-04110 [pii]
AID - 10.3390/cells11244110 [doi]
PST - epublish
SO  - Cells. 2022 Dec 17;11(24):4110. doi: 10.3390/cells11244110.