PMID- 36555134
OWN - NLM
STAT- MEDLINE
DCOM- 20221226
LR  - 20221226
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 24
DP  - 2022 Dec 7
TI  - Sex Lethal Gene Manipulates Gonadal Development of Medaka, Oryzias latipes, 
      through Estrogenic Interventions.
LID - 10.3390/ijms232415496 [doi]
LID - 15496
AB  - Germ cells are pivotal for gonadal sexuality maintenance and reproduction. Sex 
      lethal (sxl), the somatic sex determining gene of Drosophila, is the known 
      regulator and initiator of germ cell femininity in invertebrates. However, the 
      role of the Sxl homologue has rarely been investigated in vertebrates. So, we 
      used medaka to clarify the role of sxl in vertebrate gonadogenesis and sexuality 
      and identified two Sxl homologues, i.e., Sxl1a and Sxl1b. We found that sxl1a 
      specifically expresses in the primordial germ cells (PGC), ovary, (early gonia 
      and oocytes), while sxl1b distributions are ubiquitous. An mRNA overexpression of 
      sxl1a accelerated germ cell numbers in 10 DAH XY fish, and sxl1a knockdown (KD), 
      on the other hand, induced PGC mis-migration, aberrant PGC structuring and 
      ultimately caused significant germ cell reduction in XX fish. Using an in vitro 
      promoter analysis and in vivo steroid treatment, we found a strong link between 
      sxl1a and estrogenic germ cell-population maintenance. Further, using sxl1a-KD 
      and erbeta2-knockout fish, we determined that sxl1 acts through erbeta2 and controls 
      PGC sexuality. Cumulatively, our study highlights the novel role of sxl1a in germ 
      cell maintenance and sexual identity assignment and thus might become a 
      steppingstone to understanding the commonalities of animal sexual development.
FAU - Chakraborty, Tapas
AU  - Chakraborty T
AUID- ORCID: 0000-0003-1984-234X
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      819-0395, Japan.
AD  - Aqua Bioresource Innovation Center (ABRIC), Faculty of Agriculture, Kyushu 
      University, Fukuoka 819-0395, Japan.
FAU - Mohapatra, Sipra
AU  - Mohapatra S
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      819-0395, Japan.
AD  - Aqua Bioresource Innovation Center (ABRIC), Faculty of Agriculture, Kyushu 
      University, Fukuoka 819-0395, Japan.
FAU - Matsuyama, Michiya
AU  - Matsuyama M
AD  - Aqua Bioresource Innovation Center (ABRIC), Faculty of Agriculture, Kyushu 
      University, Fukuoka 819-0395, Japan.
FAU - Nagahama, Yoshitaka
AU  - Nagahama Y
AD  - National Institute of Basic Biology, Okazaki 444-8585, Japan.
FAU - Ohta, Kohei
AU  - Ohta K
AD  - Laboratory of Marine Biology, Faculty of Agriculture, Kyushu University, Fukuoka 
      819-0395, Japan.
AD  - Aqua Bioresource Innovation Center (ABRIC), Faculty of Agriculture, Kyushu 
      University, Fukuoka 819-0395, Japan.
LA  - eng
GR  - 16H04981, 19H03049, 22H00386,22K05832, 22K19211/Japan Society for the Promotion 
      of Science/
PT  - Journal Article
DEP - 20221207
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
SB  - IM
MH  - Animals
MH  - Female
MH  - *Oryzias/genetics
MH  - Genes, Lethal
MH  - Gonads
MH  - Sex Differentiation
MH  - Ovary
MH  - Germ Cells
PMC - PMC9779652
OTO - NOTNLM
OT  - estrogen
OT  - estrogen receptor
OT  - germ cell
OT  - gonad
OT  - medaka
OT  - mismigration
OT  - sex lethal
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/27 06:00
PMCR- 2022/12/07
CRDT- 2022/12/23 01:32
PHST- 2022/10/29 00:00 [received]
PHST- 2022/12/02 00:00 [revised]
PHST- 2022/12/02 00:00 [accepted]
PHST- 2022/12/23 01:32 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/27 06:00 [medline]
PHST- 2022/12/07 00:00 [pmc-release]
AID - ijms232415496 [pii]
AID - ijms-23-15496 [pii]
AID - 10.3390/ijms232415496 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Dec 7;23(24):15496. doi: 10.3390/ijms232415496.