PMID- 36555753
OWN - NLM
STAT- MEDLINE
DCOM- 20221226
LR  - 20230317
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 23
IP  - 24
DP  - 2022 Dec 17
TI  - Extracellular Vesicles as Mediators of Nickel-Induced Cancer Progression.
LID - 10.3390/ijms232416111 [doi]
LID - 16111
AB  - Emerging evidence suggests that extracellular vesicles (EVs), which represent a 
      crucial mode of intercellular communication, play important roles in cancer 
      progression by transferring oncogenic materials. Nickel (Ni) has been identified 
      as a human group I carcinogen; however, the underlying mechanisms governing 
      Ni-induced carcinogenesis are still being elucidated. Here, we present data 
      demonstrating that Ni exposure generates EVs that contribute to Ni-mediated 
      carcinogenesis and cancer progression. Human bronchial epithelial (BEAS-2B) cells 
      and human embryonic kidney-293 (HEK293) cells were chronically exposed to Ni to 
      generate Ni-treated cells (Ni-6W), Ni-transformed BEAS-2B cells (Ni-3) and 
      Ni-transformed HEK293 cells (HNi-4). The signatures of EVs isolated from Ni-6W, 
      Ni-3, HNi-4, BEAS-2B, and HEK293 were analyzed. Compared to their respective 
      untreated cells, Ni-6W, Ni-3, and HNi-4 released more EVs. This change in EV 
      release coincided with increased transcription of the EV biogenesis markers CD82, 
      CD63, and flotillin-1 (FLOT). Additionally, EVs from Ni-transformed cells had 
      enriched protein and RNA, a phenotype also observed in other studies 
      characterizing EVs from cancer cells. Interestingly, both epithelial cells and 
      human umbilical vein endothelial (HUVEC) cells showed a preference for taking up 
      Ni-altered EVs compared to EVs released from the untreated cells. Moreover, these 
      Ni-altered EVs induced inflammatory responses in both epithelial and endothelial 
      cells and increased the expression of coagulation markers in endothelial cells. 
      Prolonged treatment of Ni-alerted EVs for two weeks induced the 
      epithelial-to-mesenchymal transition (EMT) in BEAS-2B cells. This study is the 
      first to characterize the effect of Ni on EVs and suggests the potential role of 
      EVs in Ni-induced cancer progression.
FAU - Liu, Shan
AU  - Liu S
AUID- ORCID: 0000-0003-0012-7282
AD  - Division of Environmental Medicine, Department of Medicine, New York University 
      School of Medicine, New York, NY 10010, USA.
FAU - Ortiz, Angelica
AU  - Ortiz A
AUID- ORCID: 0000-0003-4008-7203
AD  - Division of Environmental Medicine, Department of Medicine, New York University 
      School of Medicine, New York, NY 10010, USA.
FAU - Stavrou, Aikaterini
AU  - Stavrou A
AUID- ORCID: 0000-0001-7922-6691
AD  - Division of Environmental Medicine, Department of Medicine, New York University 
      School of Medicine, New York, NY 10010, USA.
FAU - Talusan, Angela R
AU  - Talusan AR
AUID- ORCID: 0000-0002-3543-2132
AD  - Division of Environmental Medicine, Department of Medicine, New York University 
      School of Medicine, New York, NY 10010, USA.
FAU - Costa, Max
AU  - Costa M
AD  - Division of Environmental Medicine, Department of Medicine, New York University 
      School of Medicine, New York, NY 10010, USA.
LA  - eng
GR  - R01 ES030572/ES/NIEHS NIH HHS/United States
GR  - R01 ES030583/ES/NIEHS NIH HHS/United States
GR  - R01 CA217923/CA/NCI NIH HHS/United States
GR  - CA229234-02/NH/NIH HHS/United States
GR  - CA217923-03/NH/NIH HHS/United States
GR  - ES030572-02/NH/NIH HHS/United States
GR  - ES029359-03/NH/NIH HHS/United States
GR  - ES030583-02/NH/NIH HHS/United States
GR  - R01 CA229234/CA/NCI NIH HHS/United States
GR  - R01 ES029359/ES/NIEHS NIH HHS/United States
PT  - Journal Article
DEP - 20221217
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 7OV03QG267 (Nickel)
SB  - IM
MH  - Humans
MH  - Nickel/toxicity/metabolism
MH  - HEK293 Cells
MH  - *Neoplasms/metabolism
MH  - Human Umbilical Vein Endothelial Cells/metabolism
MH  - *Extracellular Vesicles/metabolism
MH  - Carcinogenesis/metabolism
PMC - PMC9785150
OTO - NOTNLM
OT  - cancer
OT  - extracellular vesicles
OT  - nickel
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/27 06:00
PMCR- 2022/12/17
CRDT- 2022/12/23 01:36
PHST- 2022/11/08 00:00 [received]
PHST- 2022/12/07 00:00 [revised]
PHST- 2022/12/13 00:00 [accepted]
PHST- 2022/12/23 01:36 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/27 06:00 [medline]
PHST- 2022/12/17 00:00 [pmc-release]
AID - ijms232416111 [pii]
AID - ijms-23-16111 [pii]
AID - 10.3390/ijms232416111 [doi]
PST - epublish
SO  - Int J Mol Sci. 2022 Dec 17;23(24):16111. doi: 10.3390/ijms232416111.