PMID- 36556973
OWN - NLM
STAT- MEDLINE
DCOM- 20221226
LR  - 20221226
IS  - 1648-9144 (Electronic)
IS  - 1010-660X (Print)
IS  - 1010-660X (Linking)
VI  - 58
IP  - 12
DP  - 2022 Nov 30
TI  - Assessment of Anti-Human Leukocyte Antigen (HLA)-Antibody-Dependent Humoral 
      Response in Patients before and after Lung Transplantation.
LID - 10.3390/medicina58121771 [doi]
LID - 1771
AB  - Background and Objectives: Testing for anti-human leukocyte antigen (HLA) 
      antibodies both before and after transplantation is of fundamental significance 
      for the success of lung transplantation. The aim of this study was the evaluation 
      of anti-HLA immunization of patients before and after lung transplant who were 
      subjected to qualification and transplantation. Materials and Methods: Prior to 
      the transplantation, patients were examined for the presence of IgG class 
      anti-HLA antibodies (anti-human leukocyte antigen), the so-called panel-reactive 
      antibodies (PRA), using the flow cytometry method. After the transplantation, the 
      class and specificity of anti-HLA antibodies (also IgG) were determined using 
      Luminex. Results: In the group examined, the PRA results ranged from 0.1% to 
      66.4%. Low (30%) and average (30-80%) immunization was found in only 9.7% of the 
      group examined. Presence of class I anti-HLA antibodies with MFI (mean 
      fluorescence intensity) greater than 1000 was found in 42.7% of the patients 
      examined, while class II anti-HLA antibodies were found in 38.4%. Immunization 
      levels before and after the transplantation were compared. In 10.87% of patients, 
      DSA antibodies (donor-specific antibodies) with MFI of over 1000 were found. 
      Conclusions: It seems that it is possible to confirm the correlation between pre- 
      and post-transplantation immunization with the use of the two presented methods 
      of determining IgG class anti-HLA antibodies by increasing the size of the group 
      studied and conducting a long-term observation thereof.
FAU - Stanjek-Cichoracka, Anita
AU  - Stanjek-Cichoracka A
AD  - Department of Biophysics, Faculty of Pharmaceutical Sciences in Sosnowiec, 
      Medical University of Silesia in Katowice, Jednosci 8, 41-200 Sosnowiec, Poland.
AD  - Laboratory of Transplant Immunology, Silesian Centre for Heart Diseases, 
      Szpitalna 17, 41-800 Zabrze, Poland.
FAU - Ochman, Marek
AU  - Ochman M
AUID- ORCID: 0000-0002-1410-5845
AD  - Silesian Centre for Heart Diseases, Department of Cardiac, Vascular and 
      Endovascular Surgery and Transplantology, Medical University of Silesia in 
      Katowice, Sklodowskiej-Curie 9, 41-800 Zabrze, Poland.
FAU - Chelmecka, Elzbieta
AU  - Chelmecka E
AUID- ORCID: 0000-0002-0111-438X
AD  - Department of Statistics, Faculty of Pharmaceutical Sciences in Sosnowiec, 
      Medical University of Silesia in Katowice, Ostrogorska 30, 41-200 Sosnowiec, 
      Poland.
FAU - Hrapkowicz, Tomasz
AU  - Hrapkowicz T
AUID- ORCID: 0000-0002-8945-7351
AD  - Silesian Centre for Heart Diseases, Department of Cardiac, Vascular and 
      Endovascular Surgery and Transplantology, Medical University of Silesia in 
      Katowice, Sklodowskiej-Curie 9, 41-800 Zabrze, Poland.
LA  - eng
PT  - Journal Article
DEP - 20221130
PL  - Switzerland
TA  - Medicina (Kaunas)
JT  - Medicina (Kaunas, Lithuania)
JID - 9425208
RN  - 0 (HLA Antigens)
RN  - 0 (Immunoglobulin G)
SB  - IM
MH  - Humans
MH  - HLA Antigens
MH  - *Lung Transplantation
MH  - *Kidney Transplantation
MH  - Immunoglobulin G
MH  - Graft Rejection/prevention & control
PMC - PMC9781897
OTO - NOTNLM
OT  - DSA
OT  - Luminex
OT  - PRA
OT  - anti-HLA
OT  - flow cytometry
OT  - lung transplantation
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/27 06:00
PMCR- 2022/11/30
CRDT- 2022/12/23 01:43
PHST- 2022/09/24 00:00 [received]
PHST- 2022/11/18 00:00 [revised]
PHST- 2022/11/28 00:00 [accepted]
PHST- 2022/12/23 01:43 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/27 06:00 [medline]
PHST- 2022/11/30 00:00 [pmc-release]
AID - medicina58121771 [pii]
AID - medicina-58-01771 [pii]
AID - 10.3390/medicina58121771 [doi]
PST - epublish
SO  - Medicina (Kaunas). 2022 Nov 30;58(12):1771. doi: 10.3390/medicina58121771.