PMID- 36558768
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221226
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 11
IP  - 12
DP  - 2022 Nov 28
TI  - Genetic and Antimicrobial Resistance Profiles of Mammary Pathogenic E. coli 
      (MPEC) Isolates from Bovine Clinical Mastitis.
LID - 10.3390/pathogens11121435 [doi]
LID - 1435
AB  - Mammary pathogenic E. coli (MPEC) is one of the main pathogens of environmental 
      origin responsible for causing clinical mastitis worldwide. Even though E. coli 
      are strongly associated with transient or persistent mastitis and the economic 
      impacts of this disease, the virulence factors involved in the pathogenesis of 
      MPEC remain unknown. Our aim was to characterize 110 MPEC isolates obtained from 
      the milk of cows with clinical mastitis, regarding the virulence factor-encoding 
      genes present, adherence patterns on HeLa cells, and antimicrobial resistance 
      profile. The MPEC isolates were classified mainly in phylogroups A (50.9%) and B1 
      (38.2%). None of the isolates harbored genes used for diarrheagenic E. coli 
      classification, but 26 (23.6%) and 4 (3.6%) isolates produced the aggregative or 
      diffuse adherence pattern, respectively. Among the 22 genes investigated, 
      encoding virulence factors associated with extraintestinal pathogenic E. coli 
      pathogenesis, fimH (93.6%) was the most frequent, followed by traT (77.3%) and 
      ompT (68.2%). Pulsed-field gel electrophoresis analysis revealed six pulse-types 
      with isolates obtained over time, thus indicating persistent intramammary 
      infections. The genes encoding beta-lactamases detected were as follows: bla(TEM) 
      (35/31.8%); bla(CTX-M-2)/bla(CTX-M-8) (2/1.8%); bla(CTX-M-15) and bla(CMY-2) 
      (1/0.9%); five isolates were classified as extended spectrum beta-lactamase 
      (ESBL) producers. As far as we know, papA, shf, ireA, sat and bla(CTX-M-8) were 
      detected for the first time in MPEC. In summary, the genetic profile of the MPEC 
      studied was highly heterogeneous, making it impossible to establish a common 
      genetic profile useful for molecular MPEC classification. Moreover, the detection 
      of ESBL-producing isolates is a serious public health concern.
FAU - Campos, Fernanda C
AU  - Campos FC
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Castilho, Ivana G
AU  - Castilho IG
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Rossi, Bruna F
AU  - Rossi BF
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Bonsaglia, Erika C R
AU  - Bonsaglia ECR
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Dantas, Stefani T A
AU  - Dantas STA
AUID- ORCID: 0000-0001-7839-4677
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Dias, Regiane C B
AU  - Dias RCB
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Fernandes Junior, Ary
AU  - Fernandes Junior A
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Hernandes, Rodrigo T
AU  - Hernandes RT
AUID- ORCID: 0000-0001-6695-6003
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
FAU - Camargo, Carlos H
AU  - Camargo CH
AD  - Bacteriology Division, Adolfo Lutz Institute, Sao Paulo 01246-000, Brazil.
FAU - Ribeiro, Marcio G
AU  - Ribeiro MG
AD  - Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine 
      and Animal Sciences, Sao Paulo State University, Botucatu 18618-681, Brazil.
FAU - Pantoja, Jose C F
AU  - Pantoja JCF
AD  - Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine 
      and Animal Sciences, Sao Paulo State University, Botucatu 18618-681, Brazil.
FAU - Langoni, Helio
AU  - Langoni H
AUID- ORCID: 0000-0001-5127-0762
AD  - Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine 
      and Animal Sciences, Sao Paulo State University, Botucatu 18618-681, Brazil.
FAU - Rall, Vera L M
AU  - Rall VLM
AUID- ORCID: 0000-0003-4280-5619
AD  - Department of Chemical and Biological Sciences, Institute of Biosciences, Sao 
      Paulo State University, Botucatu 18618-691, Brazil.
LA  - eng
GR  - 302383/2018-2/National Council for Scientific and Technological Development/
GR  - 001/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20221128
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC9781227
OTO - NOTNLM
OT  - ESBL
OT  - MPEC
OT  - intramammary infection
OT  - phylogroup
OT  - virulence
COIS- The authors certify that they have no affiliations with or involvement in any 
      organization or entity with any financial interest or non-financial interest in 
      the subject matter or materials discussed in this manuscript.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/11/28
CRDT- 2022/12/23 01:54
PHST- 2022/10/27 00:00 [received]
PHST- 2022/11/24 00:00 [revised]
PHST- 2022/11/25 00:00 [accepted]
PHST- 2022/12/23 01:54 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/11/28 00:00 [pmc-release]
AID - pathogens11121435 [pii]
AID - pathogens-11-01435 [pii]
AID - 10.3390/pathogens11121435 [doi]
PST - epublish
SO  - Pathogens. 2022 Nov 28;11(12):1435. doi: 10.3390/pathogens11121435.