PMID- 36558784
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221227
IS  - 2076-0817 (Print)
IS  - 2076-0817 (Electronic)
IS  - 2076-0817 (Linking)
VI  - 11
IP  - 12
DP  - 2022 Dec 1
TI  - Application of Fluorescence In Situ Hybridization (FISH) in Oral Microbial 
      Detection.
LID - 10.3390/pathogens11121450 [doi]
LID - 1450
AB  - Varieties of microorganisms reside in the oral cavity contributing to the 
      occurrence and development of microbes associated with oral diseases; however, 
      the distribution and in situ abundance in the biofilm are still unclear. In order 
      to promote the understanding of the ecosystem of oral microbiota and the 
      diagnosis of oral diseases, it is necessary to monitor and compare the oral 
      microorganisms from different niches of the oral cavity in situ. The fluorescence 
      in situ hybridization (FISH) has proven to be a powerful tool for representing 
      the status of oral microorganisms in the oral cavity. FISH is one of the most 
      routinely used cytochemical techniques for genetic detection, identification, and 
      localization by a fluorescently labeled nucleic acid probe, which can hybridize 
      with targeted nucleic acid sequences. It has the advantages of rapidity, safety, 
      high sensitivity, and specificity. FISH allows the identification and 
      quantification of different oral microorganisms simultaneously. It can also 
      visualize microorganisms by combining with other molecular biology technologies 
      to represent the distribution of each microbial community in the oral biofilm. In 
      this review, we summarized and discussed the development of FISH technology and 
      the application of FISH in oral disease diagnosis and oral ecosystem research, 
      highlighted its advantages in oral microbiology, listed the existing problems, 
      and provided suggestions for future development..
FAU - Gu, Junjie
AU  - Gu J
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Wang, Huayu
AU  - Wang H
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Zhang, Mengye
AU  - Zhang M
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Xiong, Yichen
AU  - Xiong Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Yang, Lei
AU  - Yang L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Ren, Biao
AU  - Ren B
AUID- ORCID: 0000-0003-4215-2873
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
FAU - Huang, Ruijie
AU  - Huang R
AUID- ORCID: 0000-0003-3211-518X
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China School of Stomatology, Sichuan University, Chengdu 610041, 
      China.
AD  - Department of Pediatric Dentistry, West China Hospital of Stomatology, Sichuan 
      University, Chengdu 610041, China.
LA  - eng
GR  - 82170947, 81600858, 81870778, 81991500, 81991501/National Natural Science 
      Foundation of China/
GR  - 2021YFQ0064/the Key Research and Development Projects of Science and Technology 
      Department of Sichuan Province/
GR  - 2020YJ0227/the Applied Basic Research Programs of Sichuan Province/
GR  - 2022-YF05-01401-SN/the Technology Innovation R&D Project of Chengdu/
PT  - Journal Article
PT  - Review
DEP - 20221201
PL  - Switzerland
TA  - Pathogens
JT  - Pathogens (Basel, Switzerland)
JID - 101596317
PMC - PMC9788346
OTO - NOTNLM
OT  - fluorescence in situ hybridization
OT  - microbial detection
OT  - oral microecology
OT  - oral microorganism
COIS- The authors declare that there are no competing financial interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/24 06:01
PMCR- 2022/12/01
CRDT- 2022/12/23 01:54
PHST- 2022/10/10 00:00 [received]
PHST- 2022/11/26 00:00 [revised]
PHST- 2022/11/29 00:00 [accepted]
PHST- 2022/12/23 01:54 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/24 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - pathogens11121450 [pii]
AID - pathogens-11-01450 [pii]
AID - 10.3390/pathogens11121450 [doi]
PST - epublish
SO  - Pathogens. 2022 Dec 1;11(12):1450. doi: 10.3390/pathogens11121450.