PMID- 36560606
OWN - NLM
STAT- MEDLINE
DCOM- 20230105
LR  - 20230131
IS  - 1999-4915 (Electronic)
IS  - 1999-4915 (Linking)
VI  - 14
IP  - 12
DP  - 2022 Nov 23
TI  - Chitinase-3-like Protein 1 Is Associated with Poor Virologic Control and Immune 
      Activation in Children Living with HIV.
LID - 10.3390/v14122602 [doi]
LID - 2602
AB  - Perinatally infected children living with HIV (CLWH) face lifelong infection and 
      associated inflammatory injury. Chitinase-like 3 protein-1 (CHI3L1) is expressed 
      by activated neutrophils and may be a clinically informative marker of systemic 
      inflammation in CLWH. We conducted a multi-centre, cross-sectional study of CLWH, 
      enrolled in the Early Pediatric Initiation Canadian Child Cure Cohort Study 
      (EPIC(4)). Plasma levels of CHI3L1, pro-inflammatory cytokines, and markers of 
      microbial translocation were measured by enzyme-linked immunosorbent assays. 
      Longitudinal clinical characteristics (viral load, neutrophil count, CD4+ and 
      CD8+ T-lymphocyte counts, and antiretroviral (ARV) regimen) were abstracted from 
      patient medical records. One-hundred-and-five (105) CLWH (median age 13 years, 
      62% female) were included in the study. Seventy-seven (81%) had viral suppression 
      on combination antiviral therapy (cART). The median CHI3L1 level was 25 mug/L (IQR 
      19-39). CHI3L1 was directly correlated with neutrophil count (rho = 0.22, p = 
      0.023) and inversely correlated with CD4/CD8 lymphocyte ratio (rho = -0.35, p = 
      0.00040). Children with detectable viral load had higher levels of CHI3L1 (40 
      mug/L (interquartile range, IQR 33-44) versus 24 mug/L (IQR 19-35), p = 0.0047). 
      CHI3L1 levels were also correlated with markers of microbial translocation 
      soluble CD14 (rho = 0.26, p = 0.010) and lipopolysaccharide-binding protein (rho = 
      0.23, p = 0.023). We did not detect differences in CHI3L1 between different cART 
      regimens. High levels of neutrophil activation marker CHI3L1 are associated with 
      poor virologic control, immune dysregulation, and microbial translocation in CLWH 
      on cART.
FAU - Bernard, Isabelle
AU  - Bernard I
AD  - Department of Pediatrics, University of Alberta, Edmonton, AB T6G 2R3, Canada.
FAU - Ransy, Doris G
AU  - Ransy DG
AD  - Unite d'immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, 
      Montreal, QC H3T 1C5, Canada.
FAU - Brophy, Jason
AU  - Brophy J
AUID- ORCID: 0000-0001-9154-8060
AD  - Division of Infectious Diseases, Children's Hospital of Eastern Ontario, Ottawa, 
      ON K1H 8L1, Canada.
AD  - Department of Pediatrics, University of Ottawa, Ottawa, ON K1N 6N5, Canada.
FAU - Kakkar, Fatima
AU  - Kakkar F
AD  - Department of Pediatrics, CHU Sainte-Justine, Universite de Montreal, Montreal, 
      QC H3T 1C5, Canada.
FAU - Bitnun, Ari
AU  - Bitnun A
AD  - Division of Infectious Diseases, Hospital for Sick Children, Department of 
      Pediatrics, University of Toronto, Toronto, ON M5G 1X8, Canada.
FAU - Samson, Lindy
AU  - Samson L
AD  - Division of Infectious Diseases, Children's Hospital of Eastern Ontario, Ottawa, 
      ON K1H 8L1, Canada.
FAU - Read, Stanley
AU  - Read S
AD  - Division of Infectious Diseases, Hospital for Sick Children, Department of 
      Pediatrics, University of Toronto, Toronto, ON M5G 1X8, Canada.
FAU - Soudeyns, Hugo
AU  - Soudeyns H
AUID- ORCID: 0000-0001-5857-3176
AD  - Unite d'immunopathologie Virale, Centre de Recherche du CHU Sainte-Justine, 
      Montreal, QC H3T 1C5, Canada.
AD  - Department of Microbiology, Infectiology & Immunology, Universite de Montreal, 
      Montreal, QC H3T 1J4, Canada.
FAU - Hawkes, Michael T
AU  - Hawkes MT
AUID- ORCID: 0000-0002-4122-0937
AD  - Department of Pediatrics, University of Alberta, Edmonton, AB T6G 2R3, Canada.
AD  - School of Public Health, University of Alberta, Edmonton, AB T6G 1C9, Canada.
AD  - Department of Medical Microbiology and Immunology, University of Alberta, 
      Edmonton, AB T6G 1C9, Canada.
AD  - Stollery Science Lab, Edmonton, AB T6G 1C9, Canada.
AD  - Women and Children's Health Research Institute, Edmonton, AB T6G 1C9, Canada.
FAU - Epic Study Group
AU  - Epic Study Group
LA  - eng
GR  - HIG-133051/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20221123
PL  - Switzerland
TA  - Viruses
JT  - Viruses
JID - 101509722
RN  - 0 (Chitinase-3-Like Protein 1)
RN  - 0 (CHI3L1 protein, human)
SB  - IM
MH  - Adolescent
MH  - Child
MH  - Female
MH  - Humans
MH  - Male
MH  - Antiretroviral Therapy, Highly Active
MH  - Canada
MH  - CD4 Lymphocyte Count
MH  - *Chitinase-3-Like Protein 1
MH  - Cohort Studies
MH  - Cross-Sectional Studies
MH  - *HIV Infections/diagnosis/immunology
MH  - Viral Load
PMC - PMC9786985
OTO - NOTNLM
OT  - HIV
OT  - child
OT  - chitinase-3-like protein 1
OT  - inflammation
OT  - microbial translocation
OT  - neutrophil
COIS- The authors declare no conflict of interest.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/27 06:00
PMCR- 2022/11/23
CRDT- 2022/12/23 02:05
PHST- 2022/10/10 00:00 [received]
PHST- 2022/11/17 00:00 [revised]
PHST- 2022/11/22 00:00 [accepted]
PHST- 2022/12/23 02:05 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/27 06:00 [medline]
PHST- 2022/11/23 00:00 [pmc-release]
AID - v14122602 [pii]
AID - viruses-14-02602 [pii]
AID - 10.3390/v14122602 [doi]
PST - epublish
SO  - Viruses. 2022 Nov 23;14(12):2602. doi: 10.3390/v14122602.