PMID- 36562687
OWN - NLM
STAT- MEDLINE
DCOM- 20230202
LR  - 20231213
IS  - 1532-4311 (Electronic)
IS  - 0882-0139 (Linking)
VI  - 52
IP  - 2
DP  - 2023 Feb
TI  - Sp1 Controls the Basal Level of Interleukin-34 Transcription.
PG  - 224-240
LID - 10.1080/08820139.2022.2157283 [doi]
AB  - Interleukin-34 (IL-34) is a cytokine that plays important roles at steady state 
      and in diseases. The induced or inhibited expression of IL-34 by stimuli has been 
      deeply investigated. However, the regulation of IL-34 basal expression is largely 
      unknown. The aim of this study is to investigate whether IL-34 expression is 
      regulated by a general transcription factor Specificity Protein 1 (Sp1) at 
      transcription level. By using bioinformatic software, four putative Sp1-binding 
      sites overlapping GC boxes were found in the core promoter region of IL-34. 
      Alignment of the core promoter sequences of mammalian IL-34 showed GC box-C 
      (-62/-57) and D (-11/-6) were conserved in some mammals. Luciferase assay results 
      showed that only deletion of GC box-C (-62/-57) significantly reduced luciferase 
      activities of IL-34 core promoter in SH-SY5Y cells. By using electrophoretic 
      mobility shift assay (EMSA), it was found that Sp1 specifically interacted with 
      GC box-C sequence CCCGCC (-62/-57) in the core promoter of IL-34. By using 
      chromatin immunoprecipitation (ChIP), it was discovered that Sp1 bound to the 
      core promoter of IL-34 in living cells. In addition, silencing of Sp1 expression 
      by its specific siRNA reduced IL-34 mRNA and protein levels significantly in 
      SH-SY5Y cells. Likewise, IL-34 expression was inhibited in a dose-dependent 
      manner by a Sp1 inhibitor Plicamycin. Furthermore, silencing of Sp1 also 
      downregulated mRNA and protein expression of IL-34 in GES-1 and 293T cell lines, 
      suggesting that IL-34 transcription regulated by Sp1 was not cell-type specific. 
      Taken together, these results indicate that Sp1 controls the basal level of IL-34 
      transcription.
FAU - Lin, Minggui
AU  - Lin M
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Liu, Xingyun
AU  - Liu X
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Zhang, Xinhui
AU  - Zhang X
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Wang, Huimin
AU  - Wang H
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Fang, Yu
AU  - Fang Y
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Wu, Xiaoting
AU  - Wu X
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Yin, Anqi
AU  - Yin A
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Yang, Wanqing
AU  - Yang W
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Zhang, Dong
AU  - Zhang D
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Li, Miaomiao
AU  - Li M
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Zhang, Ling
AU  - Zhang L
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
FAU - Ying, Songcheng
AU  - Ying S
AD  - Department of Immunology, School of Basic Medical Sciences, Anhui Medical 
      University, Hefei, Anhui, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20221223
PL  - England
TA  - Immunol Invest
JT  - Immunological investigations
JID - 8504629
RN  - 0 (Interleukins)
RN  - 0 (RNA, Messenger)
RN  - EC 1.13.12.- (Luciferases)
RN  - 0 (Sp1 Transcription Factor)
RN  - 0 (SP1 protein, human)
SB  - IM
MH  - Animals
MH  - Humans
MH  - *Neuroblastoma/genetics
MH  - Promoter Regions, Genetic
MH  - Binding Sites
MH  - Interleukins/genetics/metabolism
MH  - RNA, Messenger/genetics
MH  - Luciferases/genetics/metabolism
MH  - Sp1 Transcription Factor/genetics/metabolism
MH  - Gene Expression Regulation
MH  - Mammals/genetics/metabolism
OTO - NOTNLM
OT  - IL-34
OT  - Sp1
OT  - regulation
OT  - transcription
EDAT- 2022/12/24 06:00
MHDA- 2023/02/03 06:00
CRDT- 2022/12/23 09:53
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2023/02/03 06:00 [medline]
PHST- 2022/12/23 09:53 [entrez]
AID - 10.1080/08820139.2022.2157283 [doi]
PST - ppublish
SO  - Immunol Invest. 2023 Feb;52(2):224-240. doi: 10.1080/08820139.2022.2157283. Epub 
      2022 Dec 23.