PMID- 36562806
OWN - NLM
STAT- MEDLINE
DCOM- 20230206
LR  - 20230206
IS  - 1432-1106 (Electronic)
IS  - 0014-4819 (Linking)
VI  - 241
IP  - 2
DP  - 2023 Feb
TI  - Up-regulation of lncRNA NEAT1 in cerebral ischemic stroke promotes activation of 
      astrocytes by modulation of miR-488-3p/RAC1.
PG  - 395-406
LID - 10.1007/s00221-022-06519-z [doi]
AB  - We aim to research the molecular mechanism of lncRNA NEAT1 in the activation of 
      astrocytes in a cerebral ischemia-reperfusion injury model. Mouse model of 
      cerebral ischemia-reperfusion injury was constructed, and shNEAT1 was 
      transfected. The infarct area, brain water content, and neurological deficiency 
      were detected. Immunofluorescence detection and fluorescence in situ 
      hybridization (FISH) assay were processed to detect glial fibrillary acidic 
      protein (GFAP) expression. Astrocyte cells were cultured for oxygen-glucose 
      deprivation/re-oxygenation (OGD)/re-oxygenation model construction. After 
      treatment by shNEAT1, miR-488-3p mimic, miR-488-3p inhibitor, Q-PCR assay, 
      western blot and ELISA were undertaken to detect the expressions of NEAT1, 
      miR-488-3p, RAC1, inflammatory cytokines, RAC1 and GFAP. Dual luciferase reporter 
      assay and RNA-binding protein immunoprecipitation (RIP) assay were used to verify 
      the binding of NEAT1, miR-488-3p and RAC1. The expression of NEAT1 in brain 
      tissue was significantly higher than that in Sham operation group. Knockdown of 
      NEAT1 inhibited the brain damage caused by middle cerebral artery occlusion 
      (MCAO) treatment, reduced the inflammatory response, and suppressed the 
      activation of astrocytes. By constructing an in vitro OGD/R cell model, it was 
      found that NEAT1 knockdown also inhibited the activation of astrocytes caused by 
      OGD/R. Knockdown of NEAT1 caused the up-regulation of miR-488-3p and the 
      down-regulation of RAC1. Knockdown of miR-488-3p or over-expression of RAC1 
      reversed the inhibitory effect of shNEAT1 on OGD/R-induced astrocyte activation. 
      Over-expression of NEAT1 in cerebral ischemic stroke promotes activation of 
      astrocytes by modulation miR-488-3p/RAC1, which is proved in vitro. Our study may 
      provide a new idea for the diagnosis and treatment of MCAO.
CI  - (c) 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Zheng, Haijian
AU  - Zheng H
AD  - Department of General Medicine, The First Affiliated Hospital of Soochow 
      University, NO. 899 Pinghai Road, Suzhou, 215006, People's Republic of China.
FAU - Zhang, Gai
AU  - Zhang G
AD  - Department of General Medicine, The First Affiliated Hospital of Soochow 
      University, NO. 899 Pinghai Road, Suzhou, 215006, People's Republic of China.
FAU - Liu, Guanglan
AU  - Liu G
AD  - Department of General Surgery, The First Affiliated Hospital of Soochow 
      University, NO. 899 Pinghai Road, Suzhou, 215006, People's Republic of China. 
      languangliu0022@163.com.
AD  - Department of General surgery, Ganyu District People's Hospital, No.88 Haicheng 
      road, Lianyungang, 222100, People's Republic of China. languangliu0022@163.com.
FAU - Wang, Ling
AU  - Wang L
AUID- ORCID: 0000-0002-4993-3165
AD  - Department of General Medicine, The First Affiliated Hospital of Soochow 
      University, NO. 899 Pinghai Road, Suzhou, 215006, People's Republic of China. 
      sudawangling1111@163.com.
LA  - eng
PT  - Journal Article
DEP - 20221223
PL  - Germany
TA  - Exp Brain Res
JT  - Experimental brain research
JID - 0043312
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Mice
MH  - Animals
MH  - Up-Regulation
MH  - *RNA, Long Noncoding/genetics/metabolism
MH  - Astrocytes/metabolism
MH  - *Ischemic Stroke
MH  - In Situ Hybridization, Fluorescence
MH  - *MicroRNAs/genetics/metabolism
MH  - *Stroke/genetics
MH  - *Brain Ischemia/genetics
MH  - *Reperfusion Injury
MH  - Infarction, Middle Cerebral Artery
MH  - Apoptosis/genetics
OTO - NOTNLM
OT  - Activation of astrocytes
OT  - Cerebral ischemic stroke
OT  - NEAT1
OT  - RAC1
OT  - miR-488-3p
EDAT- 2022/12/24 06:00
MHDA- 2023/02/07 06:00
CRDT- 2022/12/23 11:13
PHST- 2022/07/18 00:00 [received]
PHST- 2022/12/01 00:00 [accepted]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2023/02/07 06:00 [medline]
PHST- 2022/12/23 11:13 [entrez]
AID - 10.1007/s00221-022-06519-z [pii]
AID - 10.1007/s00221-022-06519-z [doi]
PST - ppublish
SO  - Exp Brain Res. 2023 Feb;241(2):395-406. doi: 10.1007/s00221-022-06519-z. Epub 
      2022 Dec 23.