PMID- 36563106
OWN - NLM
STAT- MEDLINE
DCOM- 20221227
LR  - 20230214
IS  - 1949-0984 (Electronic)
IS  - 1949-0976 (Print)
IS  - 1949-0976 (Linking)
VI  - 15
IP  - 1
DP  - 2023 Jan-Dec
TI  - Intratumor microbiome features reveal antitumor potentials of intrahepatic 
      cholangiocarcinoma.
PG  - 2156255
LID - 10.1080/19490976.2022.2156255 [doi]
LID - 2156255
AB  - Intrahepatic cholangiocarcinoma (ICC) is a rare malignancy with a high prevalence 
      in China. This study aimed to characterize the ICC tissues' bacterial 
      metagenomics signature and explore its antitumor potential for cancer. In this 
      study, 16S rRNA sequencing was carried out on 99 tissues to characterize the 
      features of intratumoral microbiota, followed by single-cell RNA sequencing 
      (scRNA-seq) and multilevel validation. The presence of microbial DNA in tissues 
      was determined using staining, fluorescence in situ hybridization (FISH), and 
      transmission electron microscopy (TEM). A Gram-positive aerobic bacterium, 
      identified as Staphylococcus capitis, was cultured from fresh tissues. Meanwhile, 
      scRNA-seq showed that intratumoral bacteria could be present in multiple cell 
      types. Using 16S rRNA sequencing, we identified a total of 2,320,287 high-quality 
      reads corresponding to 4,594 OTU (operational taxonomic units) sequences. The 
      most abundant bacterial orders include Burkholderiales, Pseudomonadales, 
      Xanthomonadales, Bacillales and Clostridiales. Alpha and Beta diversity analysis 
      revealed specific features in different tissues. In addition, the content of 
      Paraburkholderia fungorum was significantly higher in the paracancerous tissues 
      and negatively correlated with CA199 (Carbohydrate antigen199) levels. The 
      results of in vitro and in vivo experiments suggest that P. fungorum possesses an 
      antitumor activity against tumors. Metabolomics and transcriptomics showed that 
      P. fungorum could inhibit tumor growth through alanine, aspartate and glutamate 
      metabolism. We determined the characteristic profile of the intratumoral 
      microbiota and the antitumor effect of P. fungorum in ICC.
FAU - Chai, Xiaoqiang
AU  - Chai X
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Wang, Jie
AU  - Wang J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Li, Huanping
AU  - Li H
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Gao, Chao
AU  - Gao C
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Li, Shuangqi
AU  - Li S
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Wei, Chuanyuan
AU  - Wei C
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Huang, Jianhang
AU  - Huang J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Tian, Yingming
AU  - Tian Y
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Yuan, Jian
AU  - Yuan J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Lu, Jiacheng
AU  - Lu J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Gao, Dongmei
AU  - Gao D
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Zheng, Yimin
AU  - Zheng Y
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Huang, Cheng
AU  - Huang C
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Zhou, Jian
AU  - Zhou J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Shi, Guoming
AU  - Shi G
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Ke, Aiwu
AU  - Ke A
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Liu, Feng
AU  - Liu F
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Fan, Jia
AU  - Fan J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
FAU - Cai, Jiabin
AU  - Cai J
AD  - Institutes of Biomedical Sciences, Department of Liver Surgery and 
      Transplantation, Liver Cancer Institute, Zhongshan Hospital, Key Laboratory of 
      Carcinogenesis and Cancer Invasion of Ministry of Education, Key Laboratory of 
      Medical Epigenetics and Metabolism, Fudan University, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Gut Microbes
JT  - Gut microbes
JID - 101495343
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Humans
MH  - RNA, Ribosomal, 16S/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - *Gastrointestinal Microbiome
MH  - *Microbiota/genetics
MH  - Bacteria/genetics
MH  - *Cholangiocarcinoma/genetics/pathology
MH  - Bile Ducts, Intrahepatic/pathology
MH  - *Bile Duct Neoplasms/genetics/pathology
PMC - PMC9794006
OTO - NOTNLM
OT  - ICC
OT  - P. fungorum
OT  - amino acid metabolism
OT  - bacteria
OT  - metabolomics
COIS- The authors declare no conflicts of interest that pertain to this work.
EDAT- 2022/12/24 06:00
MHDA- 2022/12/28 06:00
PMCR- 2022/12/23
CRDT- 2022/12/23 13:22
PHST- 2022/12/23 13:22 [entrez]
PHST- 2022/12/24 06:00 [pubmed]
PHST- 2022/12/28 06:00 [medline]
PHST- 2022/12/23 00:00 [pmc-release]
AID - 2156255 [pii]
AID - 10.1080/19490976.2022.2156255 [doi]
PST - ppublish
SO  - Gut Microbes. 2023 Jan-Dec;15(1):2156255. doi: 10.1080/19490976.2022.2156255.