PMID- 36565272
OWN - NLM
STAT- MEDLINE
DCOM- 20230224
LR  - 20230224
IS  - 1612-1880 (Electronic)
IS  - 1612-1872 (Linking)
VI  - 20
IP  - 2
DP  - 2023 Feb
TI  - Antileishmanial Screening, Cytotoxicity, and Chemical Composition of Essential 
      Oils: A Special Focus on Piper callosum Essential Oil.
PG  - e202200689
LID - 10.1002/cbdv.202200689 [doi]
AB  - Leishmania amazonensis is the etiological agent of tegumentary leishmaniasis, a 
      disease characterized by the emergence of cutaneous and mucocutaneous ulcerated 
      lesions that can evolve into severe destruction of skin tissue. Treatment of the 
      disease is often accompanied by high toxicity and variable efficacy. Essential 
      oils stand out for having diverse pharmacological properties. Here, we screened a 
      panel of fourteen essential oils for their anti-L. amazonensis activity, 
      cytotoxicity, and chemical profile. Lippia sidoides (LSEO) and Piper callosum 
      (PCEO) oils displayed the best anti-promastigote and anti-amastigote activities 
      with IC(50) of 31 and 21 mug/ml, respectively. PCEO was the safest oil with a 
      desirable selectivity index >10. In addition, PCEO showed no cytotoxicity against 
      the VERO line and erythrocytes. PCEO-treated amastigotes displayed mitochondrial 
      membrane depolarization and high levels of intracellular ROS. Safrole (54.72 %) 
      was the main component of PCEO. The results described here highlight the use of 
      essential oils to combat tegumentary leishmaniasis.
CI  - (c) 2022 Wiley-VHCA AG, Zurich, Switzerland.
FAU - Garcia, Andreza R
AU  - Garcia AR
AD  - Programa de Pos Graduacao em Ciencias Farmaceuticas, Faculdade de Farmacia, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
FAU - Amaral, Ana Claudia F
AU  - Amaral ACF
AUID- ORCID: 0000-0002-2532-471X
AD  - Laboratorio de Produtos Naturais e Derivados, Departamento de Produtos Naturais, 
      Farmanguinhos, FIOCRUZ, Rio de Janeiro, 22775-903, Brazil.
FAU - Maria, Ana Clara B
AU  - Maria ACB
AD  - Laboratorio de Produtos Naturais e Derivados, Departamento de Produtos Naturais, 
      Farmanguinhos, FIOCRUZ, Rio de Janeiro, 22775-903, Brazil.
FAU - Paz, Mariana M
AU  - Paz MM
AD  - Programa de Pos Graduacao em Ciencias Farmaceuticas, Faculdade de Farmacia, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
FAU - Amorim, Mariana M B
AU  - Amorim MMB
AD  - Instituto Municipal de Vigilancia Sanitaria, Vigilancia de Zoonoses e de Inspecao 
      Agropecuaria, Rio de Janeiro, 22290-240, Brazil.
FAU - Chaves, Francisco C M
AU  - Chaves FCM
AD  - EMBRAPA Amazonia Ocidental, Rodovia AM 10 km 29, Manaus, 69010-970, Brazil.
FAU - Vermelho, Alane B
AU  - Vermelho AB
AUID- ORCID: 0000-0001-5926-4172
AD  - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
FAU - Nico, Dirlei
AU  - Nico D
AUID- ORCID: 0000-0002-6129-1142
AD  - Departamento de Microbiologia Geral, Instituto de Microbiologia Paulo de Goes, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
FAU - Rodrigues, Igor A
AU  - Rodrigues IA
AUID- ORCID: 0000-0001-9432-3991
AD  - Programa de Pos Graduacao em Ciencias Farmaceuticas, Faculdade de Farmacia, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
AD  - Departamento de Produtos Naturais e Alimentos, Faculdade de Farmacia, 
      Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-902, Brazil.
LA  - eng
GR  - E-26/203.126/2017/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do 
      Rio de Janeiro/
GR  - E-26/201.362/2022/Fundacao Carlos Chagas Filho de Amparo a Pesquisa do Estado do 
      Rio de Janeiro/
GR  - 440011-2022-1/Conselho Nacional Cientifico e Tecnologico/
GR  - Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/
PT  - Journal Article
DEP - 20230116
PL  - Switzerland
TA  - Chem Biodivers
JT  - Chemistry & biodiversity
JID - 101197449
RN  - 0 (Oils, Volatile)
RN  - 0 (Antiprotozoal Agents)
SB  - IM
MH  - Humans
MH  - Animals
MH  - Mice
MH  - *Oils, Volatile/chemistry
MH  - *Leishmania
MH  - *Piper/chemistry
MH  - *Antiprotozoal Agents/chemistry
MH  - *Leishmaniasis/drug therapy
MH  - Mice, Inbred BALB C
OTO - NOTNLM
OT  - Tegumentary leishmaniasis
OT  - antileishmanial activity
OT  - drug screening
OT  - drug selectivity
OT  - volatile oils
EDAT- 2022/12/25 06:00
MHDA- 2023/02/25 06:00
CRDT- 2022/12/24 08:02
PHST- 2022/07/22 00:00 [received]
PHST- 2022/12/23 00:00 [accepted]
PHST- 2022/12/25 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2022/12/24 08:02 [entrez]
AID - 10.1002/cbdv.202200689 [doi]
PST - ppublish
SO  - Chem Biodivers. 2023 Feb;20(2):e202200689. doi: 10.1002/cbdv.202200689. Epub 2023 
      Jan 16.