PMID- 36565282
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20230201
LR  - 20230202
IS  - 1879-1123 (Electronic)
IS  - 1044-0305 (Linking)
VI  - 34
IP  - 2
DP  - 2023 Feb 1
TI  - Influence of Matrix pK(a) on Molecular Ion Formation in Matrix-Enhanced 
      Secondary-Ion Mass Spectrometry.
PG  - 218-226
LID - 10.1021/jasms.2c00261 [doi]
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is one of the most 
      important techniques for chemical imaging of nanomaterials and biological samples 
      with high lateral resolution. However, low ionization efficiency limits the 
      detection of many molecules at low concentrations or in very small volumes. One 
      promising approach to increasing the sensitivity of the technique is by the 
      addition of a matrix that promotes ionization and desorption of important analyte 
      molecules. This approach is known as matrix-enhanced secondary-ion mass 
      spectrometry (ME-SIMS). We have investigated the effect of matrix acidity on 
      molecular ion formation in three different biomolecules. A series of cinnamic 
      acid based matrixes that vary in acidity was employed to systematically 
      investigate the influence of matrix acidity on analyte ion formation. The 
      positive ion signal for all three biomolecules showed a strong increase for more 
      acidic matrixes. The most acidic matrix was then vapor-deposited onto mouse brain 
      sections. This led to significant enhancement of lipid signals from the brain. 
      This work indicates that proton donation plays an important role in the formation 
      of molecular ions in ME-SIMS.
FAU - Pohkrel, Yogesh
AU  - Pohkrel Y
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149Munster, Germany.
FAU - Adolphs, Thorsten
AU  - Adolphs T
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149Munster, Germany.
FAU - Peterson, Richard E
AU  - Peterson RE
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149Munster, Germany.
FAU - Allebrod, Ute
AU  - Allebrod U
AD  - Organic Chemistry Institute and Center for Nanotechnology (CeNTech), Westfalische 
      Wilhelms-Universitat Munster, Corrensstrasse 36, 48149Munster, Germany.
FAU - Ravoo, Bart Jan
AU  - Ravoo BJ
AUID- ORCID: 0000-0003-2202-7485
AD  - Organic Chemistry Institute and Center for Nanotechnology (CeNTech), Westfalische 
      Wilhelms-Universitat Munster, Corrensstrasse 36, 48149Munster, Germany.
FAU - Arlinghaus, Heinrich F
AU  - Arlinghaus HF
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149Munster, Germany.
FAU - Tyler, Bonnie J
AU  - Tyler BJ
AUID- ORCID: 0000-0002-3918-4869
AD  - Physikalisches Institut and Center for Soft Nanoscience, University of Munster, 
      Wilhelm-Klemm-Strasse 10, 48149Munster, Germany.
LA  - eng
PT  - Journal Article
DEP - 20221224
PL  - United States
TA  - J Am Soc Mass Spectrom
JT  - Journal of the American Society for Mass Spectrometry
JID - 9010412
SB  - IM
EDAT- 2022/12/25 06:00
MHDA- 2022/12/25 06:01
CRDT- 2022/12/24 08:42
PHST- 2022/12/25 06:00 [pubmed]
PHST- 2022/12/25 06:01 [medline]
PHST- 2022/12/24 08:42 [entrez]
AID - 10.1021/jasms.2c00261 [doi]
PST - ppublish
SO  - J Am Soc Mass Spectrom. 2023 Feb 1;34(2):218-226. doi: 10.1021/jasms.2c00261. 
      Epub 2022 Dec 24.