PMID- 36565788
OWN - NLM
STAT- MEDLINE
DCOM- 20231117
LR  - 20231117
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 562
DP  - 2023 Feb 15
TI  - Zinc finger protein 251 deficiency impairs glucose metabolism by inducing 
      adipocyte hypertrophy.
PG  - 111838
LID - S0303-7207(22)00286-6 [pii]
LID - 10.1016/j.mce.2022.111838 [doi]
AB  - Zinc finger protein (ZFP) 251 is a member of the C(2)H(2) ZFP family containing a 
      Kruppel-associated box domain that might mainly act as a transcriptional 
      repressor. However, its cellular function remains largely unknown. Here, we 
      discovered that ZFP251 deficiency caused glucose intolerance in mice. This 
      phenotype was associated with impaired insulin signaling due to hypertrophic 
      changes in white adipose tissue (WAT). Gene ontology analysis revealed that 
      ZFP251 deficiency affected the expression of genes associated with adipocyte 
      differentiation and lipid and fatty acid metabolism. Consistent with in vivo 
      results, hypertrophic changes were observed in Zfp251 knockdown (KD) 3T3-L1 
      adipocytes. In addition, Zfp251 KD 3T3-L1 preadipocytes exhibited cell cycle 
      arrest in G0/G1 phase, leading to impaired differentiation into mature 
      adipocytes, upon which abnormal mitotic clonal expansion and reduced expression 
      of adipogenic markers were exhibited. These results suggest that ZFP251 
      deficiency causes impaired adipogenesis and adipocyte hypertrophy, leading to 
      dysfunction of WAT.
CI  - Copyright (c) 2022 Elsevier B.V. All rights reserved.
FAU - Kim, Eunsu
AU  - Kim E
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Ham, Sun Ah
AU  - Ham SA
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Hwang, Jung Seok
AU  - Hwang JS
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Won, Jun Pil
AU  - Won JP
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Lee, Hyuk Gyoon
AU  - Lee HG
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Hur, Jinwoo
AU  - Hur J
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea.
FAU - Seo, Han Geuk
AU  - Seo HG
AD  - College of Sang-Huh Life Sciences, Konkuk University, 120 Neungdong-ro, 
      Gwangjin-gu, Seoul, 05029, Republic of Korea. Electronic address: 
      hgseo@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20221221
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - IY9XDZ35W2 (Glucose)
RN  - 0 (Zfp251 protein, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - 3T3-L1 Cells
MH  - *Adipocytes/metabolism
MH  - *Adipogenesis/genetics
MH  - Cell Differentiation/genetics
MH  - Glucose/metabolism
MH  - Hypertrophy/metabolism
MH  - Zinc Fingers
OTO - NOTNLM
OT  - Adipocyte hypertrophy
OT  - Adipogenesis
OT  - Glucose intolerance
OT  - Insulin resistance
OT  - ZFP251
OT  - Zinc finger protein
COIS- Declaration of competing interest The authors declare that they have no competing 
      interests.
EDAT- 2022/12/25 06:00
MHDA- 2023/01/25 06:00
CRDT- 2022/12/24 19:13
PHST- 2022/10/24 00:00 [received]
PHST- 2022/12/05 00:00 [revised]
PHST- 2022/12/20 00:00 [accepted]
PHST- 2022/12/25 06:00 [pubmed]
PHST- 2023/01/25 06:00 [medline]
PHST- 2022/12/24 19:13 [entrez]
AID - S0303-7207(22)00286-6 [pii]
AID - 10.1016/j.mce.2022.111838 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2023 Feb 15;562:111838. doi: 10.1016/j.mce.2022.111838. Epub 
      2022 Dec 21.