PMID- 36565836
OWN - NLM
STAT- MEDLINE
DCOM- 20230120
LR  - 20230120
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 228
DP  - 2023 Feb 15
TI  - Sodium butyrate protects against rotavirus-induced intestinal epithelial barrier 
      damage by activating AMPK-Nrf2 signaling pathway in IPEC-J2 cells.
PG  - 186-196
LID - S0141-8130(22)03130-0 [pii]
LID - 10.1016/j.ijbiomac.2022.12.219 [doi]
AB  - Rotavirus (RV) mainly infects intestinal epithelial cells, which leads to 
      diarrhea in newborn piglets with dysfunction in the intestinal mucosal mechanical 
      barrier. Sodium butyrate (SB) is one of the metabolites excreted by gut microbes. 
      However, the protective effect of SB on RV infection induced intestinal mucosal 
      mechanical barrier injury and its potential mechanism has not been well 
      elucidated. In the present study, IPEC-J2 cells with RV infection was a model of 
      intestinal mucosal mechanical barrier injury. Our results demonstrated that the 
      appropriate concentration of SB can effectively alleviate TJ structural damage 
      and up-regulating the expression of TJ-related genes. Furthermore, the 
      appropriate concentration of SB can effectively reverse the increase of 
      intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) level 
      induced by RV infection. Meanwhile, the levels of antioxidant enzymes superoxide 
      dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-px) and 
      antioxidant proteins NAD(P)H dehydrogenase quinone 1 (NQO1) and heme oxygenase-1 
      (HO-1) were increased through SB treatment. In addition, we found that SB 
      increased cellular antioxidant capacity by activating the adenosine 
      monophosphate-activated protein kinase (AMPK)-nuclear factor erythroid 2-related 
      factor (Nrf2) signaling pathway and the cytoprotective effect of SB is limited by 
      GPR109A siRNA. Thus, our findings revealed that SB reduces oxidative stress 
      caused by RV infection and restores the intestinal mucosal mechanical barrier 
      function by activating the AMPK-Nrf2 signal pathway mediated by the receptor 
      GPR109A.
CI  - Copyright (c) 2022 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Dong, Xia
AU  - Dong X
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Wang, Yi
AU  - Wang Y
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Zhu, Xiaoxiao
AU  - Zhu X
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Shen, Linyuan
AU  - Shen L
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Chen, Lei
AU  - Chen L
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Niu, Lili
AU  - Niu L
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Gan, Mailin
AU  - Gan M
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Zhang, Shunhua
AU  - Zhang S
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Zhang, Ming
AU  - Zhang M
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Jiang, Jun
AU  - Jiang J
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China.
FAU - Chen, Daiwen
AU  - Chen D
AD  - Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, Sichuan 
      Province 611130, PR China.
FAU - Zhu, Li
AU  - Zhu L
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China. Electronic address: zhuli@sicau.edu.cn.
FAU - Zhao, Ye
AU  - Zhao Y
AD  - College of Animal Science and Technology, Sichuan Agricultural University, 
      Chengdu, Sichuan Province 611130, PR China; Farm Animal Genetic Resources 
      Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan 
      Agricultural University, PR China. Electronic address: zhye@sicau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20221222
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Antioxidants)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 107-92-6 (Butyric Acid)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - Swine
MH  - *Antioxidants/pharmacology/metabolism
MH  - NF-E2-Related Factor 2/metabolism
MH  - *Rotavirus
MH  - Butyric Acid/pharmacology
MH  - AMP-Activated Protein Kinases/metabolism
MH  - Oxidative Stress
MH  - Signal Transduction
MH  - Epithelial Cells/metabolism
OTO - NOTNLM
OT  - IPEC-J2 cells
OT  - Intestinal mucosal mechanical barrier
OT  - Oxidative stress
OT  - Rotavirus
OT  - Sodium butyrate
COIS- Declaration of competing interest The authors declare that the research was 
      conducted in the absence of any commercial or financial relationships that could 
      be construed as a potential conflict of interest.
EDAT- 2022/12/25 06:00
MHDA- 2023/01/21 06:00
CRDT- 2022/12/24 19:14
PHST- 2022/10/27 00:00 [received]
PHST- 2022/12/18 00:00 [revised]
PHST- 2022/12/19 00:00 [accepted]
PHST- 2022/12/25 06:00 [pubmed]
PHST- 2023/01/21 06:00 [medline]
PHST- 2022/12/24 19:14 [entrez]
AID - S0141-8130(22)03130-0 [pii]
AID - 10.1016/j.ijbiomac.2022.12.219 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Feb 15;228:186-196. doi: 
      10.1016/j.ijbiomac.2022.12.219. Epub 2022 Dec 22.