PMID- 36567953
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20221227
IS  - 2699-9404 (Electronic)
IS  - 2699-9404 (Linking)
VI  - 9
IP  - 4
DP  - 2022 Dec
TI  - Differentiation and Immunological Function of MDSC-Derived Dendritic Cells.
PG  - 290-299
LID - 10.1055/s-0042-1756659 [doi]
AB  - Dendritic cells (DCs) play a key role in initiating and regulating immune 
      responses, and in addition to their roles in vivo, DCs are used as natural 
      adjuvants for various tumor vaccines. In vitro, monocytes can be used to induce 
      DCs, but in tumor patients, due to insufficient bone marrow hematopoiesis, 
      extramedullary hematopoiesis and tumor-associated myeloid cells expand, and 
      monocytes mainly exist in the form of myeloid-derived suppressor cells (MDSCs). 
      The purpose of this experiment was to explore the differences in the 
      differentiation and immune function of DCs induced by MDSCs in tumor patients. In 
      a mouse model, we used normal mouse bone marrow cell-derived DCs as control 
      cells, and in a tumor-bearing model, we induced MDSCs in the spleen to generate 
      DCs (MDSC-DCs). Through flow cytometry, we found that the production of MDSC-DCs 
      was significantly higher than that of control mice, and the secretion of 
      interferon-gamma of MDSC-DCs was significantly reduced. Through OVA antigen 
      presentation experiments, we found that the antigen presentation ability of 
      MDSC-DCs was significantly decreased. Through adoptive treatment of tumor-bearing 
      mice cells, we found that the antitumor immune function of MDSC-DCs was 
      significantly reduced. After that, we explored the mechanism of the decrease of 
      immune function activity of MDSC-DCs. We determined that the surface markers of 
      MDSC-DCs were changed by flow cytometry. Through flow sorting and RNA sequencing, 
      we found that some pathways and key gene expression in MDSC-DCs were changed. In 
      conclusion, this study found that the immune function of MDSC-DCs decreased and 
      explored the mechanism of the decreased immune function activity.
CI  - The Author(s). This is an open access article published by Thieme under the terms 
      of the Creative Commons Attribution License, permitting unrestricted use, 
      distribution, and reproduction so long as the original work is properly cited. ( 
      https://creativecommons.org/licenses/by/4.0/ ).
FAU - Ding, Zequn
AU  - Ding Z
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med-X Stem Cell 
      Research Center, Ren Ji Hospital, School of Biomedical Engineering, Shanghai Jiao 
      Tong University, Shanghai, PR China.
AD  - Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong 
      University, Shanghai, China.
FAU - Zhang, Yan
AU  - Zhang Y
AD  - State Key Laboratory of Oncogenes and Related Genes, Renji-Med-X Stem Cell 
      Research Center, Ren Ji Hospital, School of Biomedical Engineering, Shanghai Jiao 
      Tong University, Shanghai, PR China.
AD  - Med-X Research Institute, School of Biomedical Engineering, Shanghai Jiao Tong 
      University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20221221
PL  - Germany
TA  - Glob Med Genet
JT  - Global medical genetics
JID - 101769583
PMC - PMC9771685
OTO - NOTNLM
OT  - BMDCs
OT  - MDSC-DCs
OT  - antitumor activity
COIS- Conflict of Interest None declared.
EDAT- 2022/12/27 06:00
MHDA- 2022/12/27 06:01
PMCR- 2022/12/01
CRDT- 2022/12/26 03:41
PHST- 2022/05/18 00:00 [received]
PHST- 2022/06/06 00:00 [accepted]
PHST- 2022/12/26 03:41 [entrez]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2022/12/27 06:01 [medline]
PHST- 2022/12/01 00:00 [pmc-release]
AID - GMG-22-00028 [pii]
AID - 10.1055/s-0042-1756659 [doi]
PST - epublish
SO  - Glob Med Genet. 2022 Dec 21;9(4):290-299. doi: 10.1055/s-0042-1756659. 
      eCollection 2022 Dec.