PMID- 36569057
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230103
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 13
DP  - 2022
TI  - In-process real-time probiotic phenotypic strain identity tracking: The use of 
      Fourier transform infrared spectroscopy.
PG  - 1052420
LID - 10.3389/fmicb.2022.1052420 [doi]
LID - 1052420
AB  - Probiotic bacteria, capable of conferring benefits to the host, can present 
      challenges in design, development, scale-up, manufacturing, commercialization, 
      and life cycle management. Strain identification is one of the main quality 
      parameters; nevertheless, this task can be challenging since established 
      methodologies can lack resolution at the strain level for some microorganisms 
      and\or are labor-intensive and time-consuming. Fourier transform infrared 
      spectroscopy (FTIRS) has been largely used for the investigation of pathogenic 
      species in the clinical field, whereas only recently has been proposed for the 
      identification of probiotic strains. Within the probiotic industrial production, 
      bacterial strains can be subjected to stressful conditions that may affect 
      genomic and phenotypic characteristics; therefore, real-time monitoring of all 
      the sequential growth steps is requested. Considering the fast, low-cost, and 
      high-throughput features, FTIRS is an innovative and functional technology for 
      typing probiotic strains from bench-top experiments to large-scale industrial 
      production, allowing the monitoring of stability and identity of probiotic 
      strains. In this study, the discriminatory power of FTIRS was assessed for four 
      Lactiplantibacillus plantarum probiotic strains grown under different conditions, 
      including temperatures (30 and 37 degrees C) and medium (broth and agar), after 
      consecutive sub-culturing steps. A comparison between the generated spectra with 
      pulsed-field gel electrophoresis (PFGE) profiles was also performed. FTIRS was 
      not only able to distinguish the strains of L. plantarum under different growth 
      conditions but also to prove the phenotypic stability of L. plantarum type strain 
      LP-CT after six growing steps. Regardless of the growth conditions, FTIRS spectra 
      related to LP-CT constituted a unique hierarchical cluster, separated from the 
      other L. plantarum strains. These results were confirmed by a PFGE analysis. In 
      addition, based on FTIRS data, broth cultures demonstrated a higher 
      reproducibility and discriminatory power with respect to agar ones. These results 
      support the introduction of FTIRS in the probiotic industry, allowing for the 
      step-by-step monitoring of massive microbial production while also guaranteeing 
      the stability and purity of the probiotic strain. The proposed novel approach can 
      constitute an impressive improvement in the probiotic manufacturing process.
CI  - Copyright (c) 2022 Deidda, Cordovana, Bozzi Cionci, Graziano, Di Gioia and Pane.
FAU - Deidda, Francesca
AU  - Deidda F
AD  - Probiotical Research S.r.L, Novara, Italy.
FAU - Cordovana, Miriam
AU  - Cordovana M
AD  - Bruker Daltonics GmbH & Co., Bremen, Germany.
FAU - Bozzi Cionci, Nicole
AU  - Bozzi Cionci N
AD  - Department of Agricultural and Food Sciences, University of Bologna, Bologna, 
      Italy.
FAU - Graziano, Teresa
AU  - Graziano T
AD  - Probiotical Research S.r.L, Novara, Italy.
FAU - Di Gioia, Diana
AU  - Di Gioia D
AD  - Department of Agricultural and Food Sciences, University of Bologna, Bologna, 
      Italy.
FAU - Pane, Marco
AU  - Pane M
AD  - Probiotical Research S.r.L, Novara, Italy.
LA  - eng
PT  - Journal Article
DEP - 20221208
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC9772554
OTO - NOTNLM
OT  - FTIR spectroscopy
OT  - IR Biotyper(R)
OT  - Lactiplantibacillus plantarum
OT  - large-scale production
OT  - probiotics
OT  - strain typing
COIS- MP, FD, and TG were employees of Probiotical Research S.r.L, Novara (Italy). MC 
      was employee of Bruker Daltonics, Bremen (Germany). The remaining authors declare 
      that the research was conducted in the absence of any commercial or financial 
      relationships that could be construed as a potential conflict of interest.
EDAT- 2022/12/27 06:00
MHDA- 2022/12/27 06:01
PMCR- 2022/12/08
CRDT- 2022/12/26 04:05
PHST- 2022/09/23 00:00 [received]
PHST- 2022/11/15 00:00 [accepted]
PHST- 2022/12/26 04:05 [entrez]
PHST- 2022/12/27 06:00 [pubmed]
PHST- 2022/12/27 06:01 [medline]
PHST- 2022/12/08 00:00 [pmc-release]
AID - 10.3389/fmicb.2022.1052420 [doi]
PST - epublish
SO  - Front Microbiol. 2022 Dec 8;13:1052420. doi: 10.3389/fmicb.2022.1052420. 
      eCollection 2022.